Immunomagnetic enrichment and flow cytometric characterization of mouse microglia

Journal of Neuroscience Methods

Volumn 219, Issue 1, 2013, Pages 176-182

  Bedi, Supinder S ^a   Smith, Philippa ^a   Hetz, Robert A ^a,b   Xue, Hasen ^a   Cox, Charles S ^a,b,c  

^a UNIVERSITY OF TEXAS   (United States)

^b UNIVERSITY OF TEXAS MEDICAL SCHOOL   (United States)

^c TEXAS A AND M UNIVERSITY   (United States)

Author keywords

Flow cytometry; Microglia; Neuroinflammation; Traumatic brain injury

Indexed keywords

CD11B ANTIGEN; CD45 ANTIGEN; MONOCLONAL ANTIBODY;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; ASSOCIATION CORTEX; BONE MARROW CELL; BRAIN TISSUE; CELL SEPARATION; CELL SUSPENSION; CEREBELLUM; CONTROLLED STUDY; FLOW CYTOMETRY; HEMISPHERE; IMMUNE RESPONSE; IMMUNOHISTOCHEMISTRY; IMMUNOMAGNETIC SEPARATION; LEUKOCYTE; MACROPHAGE; MALE; MICROGLIA; MOUSE; NERVE CELL; NERVOUS SYSTEM INFLAMMATION; NONHUMAN; PRIORITY JOURNAL; TRAUMATIC BRAIN INJURY;

FLOW CYTOMETRY; MICROGLIA; NEUROINFLAMMATION; TRAUMATIC BRAIN INJURY;

ANIMALS; ANTIGENS, CD11B; ANTIGENS, CD45; BRAIN; BRAIN INJURIES; CELL SEPARATION; DATA INTERPRETATION, STATISTICAL; FLOW CYTOMETRY; FLUORESCENT DYES; IMMUNOHISTOCHEMISTRY; IMMUNOMAGNETIC SEPARATION; LEUKOCYTE COUNT; MALE; MICE; MICE, INBRED C57BL; MICROGLIA;

EID: 84882967223     PISSN: 01650270     EISSN: 1872678X     Source Type: Journal    
DOI: 10.1016/j.jneumeth.2013.07.017     Document Type: Article

References (21)

1. David S., Kroner A. Repertoire of microglial and macrophage responses after spinal cord injury. Nature Reviews Neuroscience 2011, 12:388-399.
2. Ford A.L., Goodsall A.L., Hickey W.F., et al. Normal adult ramified microglia separated from other central nervous system macrophages by flow cytometric sorting. Phenotypic differences defined and direct ex vivo antigen presentation to myelin basic protein-reactive CD4+ T cells compared. Journal of Immunology 1995, 154:4309-4321.
3. Gordon S. Alternative activation of macrophages. Nature Reviews Immunology 2003, 3:23-35.
4. Graeber M.B. Changing face of microglia. Science 2010, 330:783-788.
5. Guerrero A.R., Uchida K., Nakajima H., et al. Blockade of interleukin-6 signaling inhibits the classic pathway and promotes an alternative pathway of macrophage activation after spinal cord injury in mice. Journal of Neuroinflammation 2012, 9:40.
6. Jin X., Ishii H., Bai Z., et al. Temporal changes in cell marker expression and cellular infiltration in a controlled cortical impact model in adult male C57BL/6 mice. PloS ONE 2012, 7:e41892.
7. Kigerl K.A., Gensel J.C., Ankeny D.P., et al. Identification of two distinct macrophage subsets with divergent effects causing either neurotoxicity or regeneration in the injured mouse spinal cord. Journal of Neuroscience 2009, 29:13435-13444.
8. Loane D.J., Byrnes K.R. Role of microglia in neurotrauma. Neurotherapeutics 2010, 7:366-377.
9. Nakajima K., Kohsaka S. Microglia: activation and their significance in the central nervous system. Journal of Biochemistry 2001, 130:169-175.
10. Nikodemova M., Watters J.J. Efficient isolation of live microglia with preserved phenotypes from adult mouse brain. Journal of Neuroinflammation 2012, 9:147.
11. Nimmerjahn A., Kirchhoff F., Helmchen F. Resting microglial cells are highly dynamic surveillants of brain parenchyma in vivo. Science 2005, 308:1314-1318.
12. Nimmerjahn F., Ravetch J.V. Fcgamma receptors as regulators of immune responses. Nature Reviews Immunology 2008, 8:34-47.
13. Papagno L., Almeida J.R., Nemes E., et al. Cell permeabilization for the assessment of T lymphocyte polyfunctional capacity. Journal of Immunological Methods 2007, 328:182-188.
14. Pineau I., Lacroix S. Proinflammatory cytokine synthesis in the injured mouse spinal cord: multiphasic expression pattern and identification of the cell types involved. Journal of Comparative Neurology 2007, 500:267-285.
15. Pineau I., Sun L., Bastien D., Lacroix S. Astrocytes initiate inflammation in the injured mouse spinal cord by promoting the entry of neutrophils and inflammatory monocytes in an IL-1 receptor/MyD88-dependent fashion. Brain, Behavior, and Immunity 2010, 24:540-553.
16. Ransohoff R.M., Perry V.H. Microglial physiology: unique stimuli, specialized responses. Annual Review of Immunology 2009, 27:119-145.
17. Smith H.S. Activated microglia in nociception. Pain Physician 2010, 13:295-304.
18. Stirling D.P., Yong V.W. Dynamics of the inflammatory response after murine spinal cord injury revealed by flow cytometry. Journal of Neuroscience Research 2008, 86:1944-1958.
19. Streit W.J. Microglia as neuroprotective, immunocompetent cells of the CNS. Glia 2002, 40:133-139.
20. Verdier M., Jayat C., Ratinaud M.H., et al. Optimization of cell permeabilization for multiparametric flow cytometric analysis with lectin staining. Cytometry 2000, 41:55-61.
21. Walker P.A., Bedi S.S., Shah S.K., et al. Intravenous multipotent adult progenitor cell therapy after traumatic brain injury: modulation of the resident microglia population. Journal of Neuroinflammation 2012, 9:228.