Optimization of cell permeabilization for multiparametric flow cytometric analysis with lectin staining

Cytometry

Volumn 41, Issue 1, 2000, Pages 55-61

  Verdier, Mireille ^a   Jayat, Chantal ^a   Ratinaud, Marie Helène ^a   Troutaud, Danielle ^a  

^a UNIVERSITY OF LIMOGES   (France)

Author keywords

Bcl 2; Lectin staining; Lymphocytes; Murine germinal centers; Permeabilization

Indexed keywords

LECTIN; PEANUT AGGLUTININ; PROTEIN BCL 2;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CELL MEMBRANE PERMEABILITY; CELL SURFACE; FEMALE; FLOW CYTOMETRY; GERMINAL CENTER; IMMUNOFLUORESCENCE; LYMPHOID CELL; MOUSE; NONHUMAN; PEYER PATCH; PRIORITY JOURNAL; SPLEEN;

ANIMALS; CELL MEMBRANE PERMEABILITY; CYTOPLASM; FEMALE; FLOW CYTOMETRY; FLUORESCENT ANTIBODY TECHNIQUE; GERMINAL CENTER; MEMBRANE PROTEINS; METHANOL; MICE; MICE, INBRED BALB C; PEANUT AGGLUTININ; PROTO-ONCOGENE PROTEINS C-BCL-2; PROTO-ONCOGENE PROTEINS C-KIT; SAPONINS; SOLVENTS; SPLEEN; STAINING AND LABELING;

EID: 0034283770     PISSN: 01964763     EISSN: None     Source Type: Journal    
DOI: 10.1002/1097-0320(20000901)41:1<55::AID-CYTO8>3.0.CO;2-A     Document Type: Article

References (28)

1. Mechanisms and genes of cellular suicide
2. Programmed cell death in animal development
3. Developmental regulation of the Bcl-2 protein and susceptibility to cell death in B lymphocytes
4. Apoptosis and programmed cell death in immunity
5. Antigen-induced B-cell death and elimination during germinal-centre immune responses
6. The Bcl-2 protein family: Arbiters of cell survival
7. The proto-oncogene Bcl-2 and its role in regulating apoptosis
8. BCL2 protein is topographically restricted in tissues characterized by apoptotic cell death
9. Germinal center cells express bcl-2 protein after activation by signals which prevent their entry into apoptosis
10. Human germinal center B cells express the apoptosis-inducing genes Fas, c-myc, P53, and Bax but not the survival gene bcl-2
11. Regulation of B cell apoptosis by Bcl-2 and Bcl-XL and its role in the development of autoimmune diseases
12. Molecular genetic features reflecting the preference for isotype switching to IgA expression by Peyer's patch germinal center B cells
13. Analysis of somatic hypermutation in mouse Peyer's patches using immunoglobulin k light-chain transgenes
14. B220: A B cell-specific member of the T200 glycoprotein family
15. Phenotype and proliferation of early B lymphocyte precursor cells in mouse bone marrow
16. Surface phenotype of Peyer's patch germinal center cells: Implications for the role of germinal centers in B cell differentiation
17. Relationship of germinal centers in lymphoid tissue to immunologic memory. VI. Transfer of B cell memory with lymph node cells fractionated according to their receptors for peanut agglutinin
18. Methanol fixation permits flow cytometric analysis of immunofluorescent stained intracellular antigens
19. Comparative study of five commercial reagents for preparing normal and leukaemic lymphocytes for immunophenotypic analysis by flow cytometry
20. Simultaneous paired analysis by flow cytometry of surface markers, cytoplasmic antigens, or oncogene expression with DNA content
21. Identification of live cells for flow cytometric analysis of lymphoid subset proliferation in low viability, populations
22. Membrane cell permeabilisation with saponin and multiparametric analysis by flow cytometry
23. Peripheral B cell maturation. I. Immature peripheral B cells in adults are heat-stable antigenhi and exhibit unique signaling characteristics
24. Characterization of immature B cells by a novel monoclonal antibody, by turnover and by mitogen reactivity
25. Expression of Bcl-2 and Bax in cultured normal human keratinocytes and melanocytes: Relationship to differentiation and melano genesis
26. Lectin receptors as lymphocyte surface markers
27. Germinal center development
28. Human naive B cells cultured with EL-4 T cells mimic a germinal center-related B cell stage before generating plasma cells. Concordant changes in Bcl-2 protein and messenger RNA levels