Role of PKCα activation of Src, PI-3K/AKT, and ERK in EGF-stimulated proliferation of rat and human conjunctival goblet cells

Investigative Ophthalmology and Visual Science

Volumn 54, Issue 8, 2013, Pages 5661-5674

  Li, Dayu ^a   Shatos, Marie A ^a   Hodges, Robin R ^a   Dartt, Darlene A ^a  

^a SCHEPENS EYE RESEARCH INSTITUTE   (United States)

Author keywords

Cell proliferation; Conjunctiva; EGF; Goblet cells

Indexed keywords

EPIDERMAL GROWTH FACTOR; MITOGEN ACTIVATED PROTEIN KINASE; MITOGEN ACTIVATED PROTEIN KINASE 1; MITOGEN ACTIVATED PROTEIN KINASE 3; PROTEIN KINASE B; PROTEIN KINASE C ALPHA; SMALL INTERFERING RNA; WORTMANNIN;

ANIMAL CELL; ARTICLE; CELL PROLIFERATION; CONJUNCTIVA; CONTROLLED STUDY; GOBLET CELL; HUMAN; HUMAN CELL; HUMAN TISSUE; MALE; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; RADIOIMMUNOPRECIPITATION; RAT; WESTERN BLOTTING;

CELL PROLIFERATION; CONJUNCTIVA; EGF; GOBLET CELLS;

ANIMALS; CELL PROLIFERATION; CHROMONES; CONJUNCTIVA; ENZYME INHIBITORS; EPIDERMAL GROWTH FACTOR; GOBLET CELLS; HUMANS; MALE; MAP KINASE SIGNALING SYSTEM; MITOGEN-ACTIVATED PROTEIN KINASE 1; MORPHOLINES; ORGAN CULTURE TECHNIQUES; PHOSPHATIDYLINOSITOL 3-KINASES; PHOSPHORYLATION; PRIMARY CELL CULTURE; PROTEIN KINASE C-ALPHA; PROTO-ONCOGENE PROTEINS C-AKT; PROTO-ONCOGENE PROTEINS PP60(C-SRC); RATS; RATS, SPRAGUE-DAWLEY; RNA, SMALL INTERFERING;

EID: 84882745525     PISSN: 01460404     EISSN: 15525783     Source Type: Journal    
DOI: 10.1167/iovs.13-12473     Document Type: Article

References (29)

1. Blume-Jensen P, Hunter T. Oncogenic kinase signalling. Nature. 2001;411:355-365.
2. Hynes NE, Lane HA. ERBB receptors and cancer: the complexity of targeted inhibitors. Nat Rev Cancer. 2005;5: 341-354.
3. Schlessinger J. Cell signaling by receptor tyrosine kinases. Cell. 2000;103:211-225.
4. Sorkin A, Goh LK. Endocytosis and intracellular trafficking of ErbBs. Exp Cell Res. 2009;315:683-696.
5. Shatos MA, Hodges RR, Oshi Y, et al. Role of cPKCalpha and nPKCepsilon in EGF-stimulated goblet cell proliferation. Invest Ophthalmol Vis Sci. 2009;50:614-620.
6. Shatos MA, Gu J, Hodges RR, Lashkari K, Dartt DA. ERK/ p44p42 mitogen-activated protein kinase mediates EGF-stimulated proliferation of conjunctival goblet cells in culture. Invest Ophthalmol Vis Sci. 2008;49:3351-3359.
7. Gu J, Chen L, Shatos MA, et al. Presence of EGF growth factor ligands and their effects on cultured rat conjunctival goblet cell proliferation. Exp Eye Res. 2008;86:322-334.
8. Hodges RR, Bair JA, Carozza RB, Li D, Shatos MA, Dartt DA. Signaling pathways used by EGF to stimulate conjunctival goblet cell secretion. Exp Eye Res. 2012;103:99-113.
9. Shatos MA, Hodges RR, Bair JA, Lashkari K, Dartt DA. Stimulatory role of PKCalpha in extracellular regulated kinase 1/2 pathway in conjunctival goblet cell proliferation. Invest Ophthalmol Vis Sci. 2009;50:1619-1625.
10. Franke TF. PI3K/Akt: getting it right matters. Oncogene. 2008; 27:6473-6488.
11. Yue Y, Lypowy J, Hedhli N, Abdellatif M. Ras GTPase-activating protein binds to Akt and is required for its activation. J Biol Chem. 2004;279:12883-12889.
12. Roskoski R Jr. Src kinase regulation by phosphorylation and dephosphorylation. Biochem Biophys Res Commun. 2005; 331:1-14.
13. Shatos MA, Kano H, Rubin P, Garza G, Dartt DA. Isolation and characterization of human goblet cells in vitro: regulation of proliferation and activation of mitogen-activated protein kinase by EGF and carbachol. Adv Exp Med Biol. 2002;506: 301-305.
14. Shatos MA, Rios JD, Tepavcevic V, Kano H, Hodges R, Dartt DA. Isolation, characterization, and propagation of rat conjunctival goblet cells in vitro. Invest Ophthalmol Vis Sci. 2001;42:1455-1464.
15. Hodges RR, Raddassi I, Zoukhri D, Toker A, Kazlauskas A, Dartt DA. Effect of overexpression of constitutively active PKCalpha on rat lacrimal gland protein secretion. Invest Ophthalmol Vis Sci. 2004;45:3974-3981.
16. Kawanabe Y, Hashimoto N, Masaki T. Effects of nonselective cation channels and PI3K on endothelin-1-induced PYK2 tyrosine phosphorylation in C6 glioma cells. Am J Physiol Cell Physiol. 2003;285:C539-C545.
17. Aksamitiene E, Kiyatkin A, Kholodenko BN. Cross-talk between mitogenic Ras/MAPK and survival PI3K/Akt pathways: a fine balance. Biochem Soc Trans. 2012;40:139-146.
18. Hodges RR, Horikawa Y, Rios JD, Shatos MA, Dartt DA. Effect of protein kinase C and Ca(2+) on p42/p44 MAPK, Pyk2, and Src activation in rat conjunctival goblet cells. Exp Eye Res. 2007;85:836-844.
19. Kanno H, Horikawa Y, Hodges RR, et al. Cholinergic agonists transactivate EGFR and stimulate MAPK to induce goblet cell secretion. Am J Physiol Cell Physiol. 2003;284:C988-C998.
20. Lu WY, Xiong ZG, Lei S, et al. G-protein-coupled receptors act via protein kinase C and Src to regulate NMDA receptors. Nat Neurosci. 1999;2:331-338.
21. Sasaki T, Suzuki A, Sasaki J, Penninger JM. Phosphoinositide 3-kinases in immunity: lessons from knockout mice. J Biochem. 2002;131:495-501.
22. Mahajan K, Mahajan NP. PI3K-independent AKT activation in cancers: a treasure trove for novel therapeutics. J Cell Physiol. 2012;227:3178-3184.
23. Choudhury GG, Mahimainathan L, Das F, Venkatesan B, Ghosh-Choudhury N. c-Src couples PI 3 kinase/Akt and MAPK signaling to PDGF-induced DNA synthesis in mesangial cells. Cell Signal. 2006;18:1854-1864.
24. Nyga R, Pecquet C, Harir N, et al. Activated STAT5 proteins induce activation of the PI 3-kinase/Akt and Ras/MAPK pathways via the Gab2 scaffolding adapter. Biochem J. 2005; 390:359-366.
25. Zugasti O, Rul W, Roux P, et al. Raf-MEK-Erk cascade in anoikis is controlled by Rac1 and Cdc42 via Akt. Mol Cell Biol. 2001; 21:6706-6717.
26. Fry MJ. Phosphoinositide (PI) 3-kinase assays. Methods Mol Biol. 2009;462:345-362.
27. Horikawa Y, Shatos MA, Hodges RR, et al. Activation of mitogen-activated protein kinase by cholinergic agonists and EGF in human compared with rat cultured conjunctival goblet cells. Invest Ophthalmol Vis Sci. 2003;44:2535-2544.
28. Dartt DA, Hodges RR, Li D, Shatos MA, Lashkari K, Serhan CN. Conjunctival goblet cell secretion stimulated by leukotrienes is reduced by resolvins D1 and E1 to promote resolution of inflammation. J Immunol. 2011;186:4455-4466.
29. Li D, Hodges RR, Jiao J, et al. Resolvin D1 and aspirin-triggered resolvin D1 regulate histamine-stimulated conjunctival goblet cell secretion. Mucosal Immunol. In press.