High-level heterologous expression of fungal cytochrome P450s in Escherichia coli

Biochemical and Biophysical Research Communications

Volumn 438, Issue 2, 2013, Pages 289-294

  Ichinose, Hirofumi ^a   Wariishi, Hiroyuki ^a  

^a KYUSHU UNIVERSITY   (Japan)

Author keywords

Escherichia coli; Eukaryotic cytochrome P450; Heterologous expression; High level expression; Large scale screening; Wood rotting basidiomycetes

Indexed keywords

CYTOCHROME P450; FUNGAL PROTEIN;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; ENTEROBACTERIALES; ESCHERICHIA COLI; GENE DELETION; HETEROLOGOUS EXPRESSION; HYDROPHOBICITY; NONHUMAN; PRIORITY JOURNAL; SCREENING; STRUCTURE ACTIVITY RELATION; WILD TYPE;

2-[4-(2-HYDROXYETHYL)-1-PIPERAZINYL]ETHANESULFONIC ACID; CYTOCHROME P450 MONOOXYGENASE; EDTA; ESCHERICHIA COLI; ETHYLENEDIAMINETETRAACETIC ACID; EUKARYOTIC CYTOCHROME P450; HEPES; HETEROLOGOUS EXPRESSION; HIGH-LEVEL EXPRESSION; IPTG; ISOPROPYL-1-THIO-Β-D-GALACTOPYRANOSIDE; LARGE-SCALE SCREENING; P450; PHENYLMETHYLSULFONYL FLUORIDE; PMSF; TMD; TRANSMEMBRANE DOMAIN; WOOD-ROTTING BASIDIOMYCETES;

AMINO ACID SEQUENCE; CYTOCHROME P-450 ENZYME SYSTEM; DNA, COMPLEMENTARY; ESCHERICHIA COLI; FUNGAL PROTEINS; MOLECULAR SEQUENCE DATA; PLASMIDS; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID;

BASIDIOMYCOTA; ESCHERICHIA COLI; EUKARYOTA;

EID: 84882720783     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2013.07.057     Document Type: Article

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