Importance of a proline-rich sequence in the amino-terminal region for correct folding of mitochondrial and soluble microbial P450s

Journal of Biochemistry

Volumn 129, Issue 2, 2001, Pages 271-277

  Kusano, Kazutomi ^a,c   Kagawa, Norio ^a   Sakaguchi, Masao ^b   Omura, Tsuneo ^a   Waterman, Michael R ^a  

^a VANDERBILT UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b KYUSHU UNIVERSITY   (Japan)

^c EISAI CO LTD   (Japan)

Author keywords

Escherichia coli; Folding; Mitachondrial P450; Proline rich sequence; Soluble microbial P450

Indexed keywords

CYTOCHROME C2; CYTOCHROME P450; HEME; MICROSOME ENZYME; PROLINE;

AMINO TERMINAL SEQUENCE; ARTICLE; CONTROLLED STUDY; ESCHERICHIA COLI; GENE EXPRESSION SYSTEM; HUMAN; MITOCHONDRIAL RESPIRATION; NONHUMAN; PROTEIN BINDING; PROTEIN EXPRESSION; PROTEIN FOLDING; SEQUENCE ANALYSIS;

BOVINAE; ESCHERICHIA COLI;

EID: 0035113534     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a002854     Document Type: Article

References (19)

1. Synthesis and insertion of cytochrome P-450 into endoplasmic reticulum membranes
2. Signal recognition particle is required for co-translational insertion of cytochrome P-450 into microsomal membranes
3. Importance of the proline-rich region following signal-anchor sequence in the formation of correct conformation of microsomal cytochrome P-450s
4. Malfolded cytochrome P-450(M1) localized in unusual membrane structures of the endoplasmic reticulum in cultured animal cells
5. Deletion of a conserved tetrapeptide, PPGP, in P450 2C2 results in loss of enzymatic activity without a change in its cellular location
6. Different structural requirements at specific proline residue positions in the conserved proline-rich region of cytochrome P450 2C2
7. A conserved proline-rich sequence between the N-terminal signal-anchor and catalytic domains is required for assembly of functional cytochrome P450 2C2
8. Microsomal P450s use specific proline-rich sequences for efficient folding, but not for maintenance of the folded structure
9. Presence of a prepiece at the NH2-terminal end of pre-cytochrome P-450-(SCC) peptide
10. Molecular cloning and nucleotide sequence of cDNA for mRNA of mitochondrial cytochrome P-450(SCC) of bovine adrenal cortex
11. Mitochondrial presequences
12. Processing-independent in vitro translocation of cytochrome P-450(SCC) precursor across mitochondrial membranes
13. Purification and characterization of a processing protease from rat liver mitochondria
14. Identification by site-directed mutagenesis of two lysine residues in cholesterol side chain cleavage cytochrome P450 that are essential for adrenodoxin binding
15. Expression, purification, and enzymatic properties of recombinant human cytochrome P450c27 (CYP27)
16. Expression and nitrogen-15 labeling of proteins for proton and nitrogen-15 nuclear magnetic resonance
17. Protein sythesis inhibitors and ethanol selectively enhance heterologous expression of P450s and related proteins in Escherichia coli
18. Design of a novel P450: A functional bacterial-human cytochrome P450 chimera