High-level expression of recombinant rabbit cytochrome P450 2E1 in Escherichia coli C41 and its purification

Protein Expression and Purification

Volumn 33, Issue 1, 2004, Pages 66-71

  Cheng, Dongmei ^a   Kelley, Rusty W ^a   Cawley, George F ^a   Backes, Wayne L ^a  

^a LOUISIANA STATE UNIVERSITY HEALTH SCIENCES CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ESCHERICHIA COLI; ORYCTOLAGUS CUNICULUS;

EID: 0348226986     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2003.08.009     Document Type: Article

References (22)

1. D.R. Koop, E.T. Morgan, G.E. Tarr, M.J. Coon, Purification and characterization of a unique isozyme of cytochrome P-450 from liver microsomes of ethanol-treated rabbits, J. Biol. Chem. 257 (1982) 8472-8480.
2. C.J. Patten, S.M. Ning, A.Y.H. Lu, C.S. Yang, Acetone-inducible cytochrome P-450: purification, catalytic activity, and interaction with cytochrome b5, Arch. Biochem. Biophys. 251 (1986) 629-638.
3. P. Puccini, S. Menicagli, V. Longo, A. Santucci, P.G. Gervasi, Purification and characterization of an acetone-inducible cytochrome P-450 from hamster liver microsomes, Biochem. J. 287 (1992) 863-870.
4. S.A. Wrighton, P.E. Thomas, D.E. Ryan, W. Levin, Purification and characterization of ethanol-inducible human hepatic cytochrome P-450 HLj, Arch. Biochem. Biophys. 258 (1987) 292-297.
5. 2-terminal segment retains catalytic activity and is membrane-bound when expressed in Escherichia coli, J. Biol. Chem. 266 (1991) 7321-7324.
6. E.M.J. Gillam, Z. Guo, F.P. Guengerich, Expression of modified human cytochrome P450 2E1 in Escherichia coli, purification, and spectral and catalytic properties, Arch. Biochem. Biophys. 312 (1994) 59-66.
7. C.J. Patten, P. Koch, Baculovirus expression of human P450 2E1 and cytochrome b5: spectral and catalytic properties and effect of b5 on the stoichiometry of P450 2E1-catalyzed reactions, Arch. Biochem. Biophys. 317 (1995) 504-513.
8. C.J. Patten, H. Ishizaki, T. Aoyama, M. Lee, S.M. Ning, W. Huang, F.J. Gonzalez, C.S. Yang, Catalytic properties of the human cytochrome P450 2E1 produced by cDNA expression in mammalian cells, Arch. Biochem. Biophys. 299 (1992) 163-171.
9. W. Chen, R.M. Peter, S. McArdle, K.E. Thummel, R.O. Sigle, S.D. Nelson, Baculovirus expression and purification of human and rat cytochrome P450 2E1, Arch. Biochem. Biophys. 335 (1996) 123-130.
10. D. Porter, J.R. Larson, Expression of mammalian P450s in Escherichia coli, Methods Enzymol. 206 (1991) 108-116.
11. B. Miroux, J.E. Walker, Overproduction of proteins in Escherichia coli: mutant hosts that allow synthesis of some membrane proteins and globular proteins at high levels, J. Mol. Biol. 260 (1996) 289-298.
12. S.B. Mulrooney, L. Waskell, High-level expression in Escherichia coli and purification of the membrane-bound form of cytochrome b5, Protein Expr. Purif. 19 (2000) 173-178.
13. W.L. Backes, C.E. Reker-Backes, The effect of NADPH concentration on the reduction of cytochrome P-450 LM2, J. Biol. Chem. 263 (1988) 247-253.
14. A.S. Saribas, L. Gruenke, L. Waskell, Overexpression and purification of the membrane-bound cytochrome P450 2B4, Protein Expr. Purif. 21 (2001) 303-309.
15. C.S. Yang, C.J. Patten, H. Ishizaki, J.S.H. Yoo, Induction, purification and characterization of cytochrome P450 IIE, Methods Enzymol. 206 (1991) 595-603.
16. K.M. Causey, C.S. Eyer, W.L. Backes, Dual role of phospholipid in the reconstitution of cytochrome P450 LM2-dependent activities, Mol. Pharmacol. 38 (1990) 134-142.
17. O.H. Lowry, N.J. Rosebrough, A.L. Farr, R.J. Randell, Protein measurement with the Folin phenol reagent, J. Biol. Chem. 193 (1951) 265-275.
18. U.K. Laemmli, Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature 227 (1970) 680-685.
19. T. Omura, R. Sato, The carbon monoxide-binding pigment of liver microsomes, J. Biol. Chem. 239 (1964) 2370-2378.
20. A. Bridges, L.D. Gruenke, Y.T. Chang, I.A. Vasker, G. Loew, L. Waskell, Identification of the binding site on cytochrome P450 2B4 for cytochrome b5 and cytochrome P450 reductase, J. Biol. Chem. 273 (1998) 17036-17049.
21. 2-terminal membrane-insertion signal peptide does not alter the catalytic activities, Proc. Natl. Acad. Sci. USA 88 (1991) 9141-9145.
22. F.P. Guengerich, M.V. Martin, Z. Guo, Y.J. Chun, Purification of functional recombinant P450s from bacteria, Methods Enzymol. 272 (1996) 35-44.