Membrane integration of recombinant human P450 forms

Xenobiotica

Volumn 39, Issue 7, 2009, Pages 495-507

  Shukla, A ^a   Huang, W ^a   Depaz, I M ^a   Gillam, E M J ^a  

^a UNIVERSITY OF QUEENSLAND   (Australia)

Author keywords

Heterologous expression; Membrane integration; Membrane proteins; Recombinant P450s; Subcellular localization

Indexed keywords

CYTOCHROME P450; CYTOCHROME P450 2A; CYTOCHROME P450 2C; CYTOCHROME P450 2C19; CYTOCHROME P450 2C9; CYTOCHROME P450 2D6; CYTOCHROME P450 2E1; DETERGENT; DICLOFENAC; LUCIFERIN; MEMBRANE PROTEIN; OXIDOREDUCTASE; RECOMBINANT ENZYME; RECOMBINANT PROTEIN; CYP2C19 PROTEIN, HUMAN; CYP2C9 PROTEIN, HUMAN; UNSPECIFIC MONOOXYGENASE;

AMINO TERMINAL SEQUENCE; ARTICLE; CELL FRACTIONATION; CELL MEMBRANE; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME METABOLISM; ENZYME MODIFICATION; ENZYME SUBSTRATE; FLUORESCENCE MICROSCOPY; HUMAN; HYDROXYLATION; IMMUNOBLOTTING; OXIDATION REDUCTION REACTION; AMINO ACID SEQUENCE; BIOSYNTHESIS; ENZYME SPECIFICITY; ENZYMOLOGY; GENE DELETION; GENETICS;

AMINO ACID SEQUENCE; ARYL HYDROCARBON HYDROXYLASES; CELL MEMBRANE; HUMANS; RECOMBINANT PROTEINS; SEQUENCE DELETION; SUBSTRATE SPECIFICITY;

EID: 70349577507     PISSN: 00498254     EISSN: 13665928     Source Type: Journal    
DOI: 10.1080/00498250902934884     Document Type: Article

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34. Kinobe RT, Parkinson OT, Mitchell DJ, Gillam EMJ. (2005). P4502C18 catalyzes the metabolic bioactivation of phenytoin. Chem Res Toxicol 18:1868-1875
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38. Lee HC, Bernstein HD. (2001). The targeting pathway of Escherichia coli presecretory and integral membrane proteins is specified by the hydrophobicity of the targeting signal. Proc Natl Acad Sci USA 98: 3471-3476
39. Leemann T, Transom C, Dayer P. (1993). Cytochrome P450 TB (CYP2C): A major monooxygenase catalyzing diclofenac 4́-hydroxylation in human liver. Life Sci 52: 29-34.
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81. Li YC, Chiang JYL. (1991). The expression of a catalytically active cholesterol 7α-hydroxylase cytochrome P-450 in Escherichia coli. J Biol Chem 266:19186-19191
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83. Luirink J, Von Heijne G, Houben E, De Gier J-W. (2005). Biogenesis of inner membrane proteins in Escherichia coli. Ann Rev Microbiol 59:329-355
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