-
1
-
-
18244380348
-
High-density miniaturized thermal shift assays as a general strategy for drug discovery
-
DOI 10.1089/108705701753364922
-
Pantoliano, M. W. et al. High-density miniaturized thermal shift assays as a general strategy for drug discovery. J. Biomol. Screen. 6, 429-440 (2001). (Pubitemid 34052847)
-
(2001)
Journal of Biomolecular Screening
, vol.6
, Issue.6
, pp. 429-440
-
-
Pantoliano, M.W.1
Petrella, E.C.2
Kwasnoski, J.D.3
Lobanov, V.S.4
Myslik, J.5
Graf, E.6
Carver, T.7
Asel, E.8
Springer, B.A.9
Lane, P.10
Salemme, F.R.11
-
2
-
-
15944380354
-
High-throughput measurement of protein stability in microtiter plates
-
DOI 10.1002/bit.20397
-
Aucamp, J. P., Cosme, A. M., Lye, G. J. & Dalby, P. A. High-throughput measurement of protein stability in microtiter plates. Biotechnol. Bioeng. 89, 599-607 (2005). (Pubitemid 40444814)
-
(2005)
Biotechnology and Bioengineering
, vol.89
, Issue.5
, pp. 599-607
-
-
Aucamp, J.P.1
Cosme, A.M.2
Lye, G.J.3
Dalby, P.A.4
-
3
-
-
79959451524
-
Leveraging the contribution of thermodynamics in drug discovery with the help of fluorescence-based thermal shift assays
-
Hau, J. C. et al. Leveraging the contribution of thermodynamics in drug discovery with the help of fluorescence-based thermal shift assays. J. Biomol. Screen. 16, 552-556 (2011).
-
(2011)
J. Biomol. Screen.
, vol.16
, pp. 552-556
-
-
Hau, J.C.1
-
4
-
-
78650485354
-
Thermodynamic analysis of ligand-induced changes in protein thermal unfolding applied to high-throughput determination of ligand affinities with extrinsic fluorescent dyes
-
Layton, C. J. & Hellinga, H. W. Thermodynamic analysis of ligand-induced changes in protein thermal unfolding applied to high-throughput determination of ligand affinities with extrinsic fluorescent dyes. Biochemistry 49, 10831-10841 (2010).
-
(2010)
Biochemistry
, vol.49
, pp. 10831-10841
-
-
Layton, C.J.1
Hellinga, H.W.2
-
5
-
-
33751209173
-
High-throughput screening for enhanced protein stability
-
DOI 10.1016/j.copbio.2006.10.001, PII S0958166906001455
-
Bommarius, A. S., Broering, J. M., Chaparro-Riggers, J. F. & Polizzi, K. M. High-throughput screening for enhanced protein stability. Curr. Opin. Biotechnol. 17, 606-610 (2006). (Pubitemid 44791907)
-
(2006)
Current Opinion in Biotechnology
, vol.17
, Issue.6
, pp. 606-610
-
-
Bommarius, A.S.1
Broering, J.M.2
Chaparro-Riggers, J.F.3
Polizzi, K.M.4
-
6
-
-
79951674715
-
High-throughput process development for biopharmaceutical drug substances
-
Bhambure, R., Kumar, K. & Rathore, A.S. High-throughput process development for biopharmaceutical drug substances. Trends Biotechnol. 29, 127-135 (2011).
-
(2011)
Trends Biotechnol.
, vol.29
, pp. 127-135
-
-
Bhambure, R.1
Kumar, K.2
Rathore, A.S.3
-
7
-
-
79957998371
-
Protein stability by number: High-throughput and statistical approaches to one of protein science's most difficult problems
-
Magliery, T. J., Lavinder, J. J. & Sullivan, B. J. Protein stability by number: high-throughput and statistical approaches to one of protein science's most difficult problems. Curr. Opin. Chem. Biol. 15, 443-451 (2011).
-
(2011)
Curr. Opin. Chem. Biol.
, vol.15
, pp. 443-451
-
-
Magliery, T.J.1
Lavinder, J.J.2
Sullivan, B.J.3
-
8
-
-
77955120354
-
Protein denaturation and protein:Drugs interactions from intrinsic protein fluorescence measurements at the nanolitre scale
-
Gaudet, M., Remtulla, N., Jackson, S. E., Main, E. R. G., Bracewell, D. G., Aeppli, G. & Dalby, P. A. Protein denaturation and protein:drugs interactions from intrinsic protein fluorescence measurements at the nanolitre scale. Protein Sci. 19, 1544-1554 (2010).
-
(2010)
Protein Sci.
, vol.19
, pp. 1544-1554
-
-
Gaudet, M.1
Remtulla, N.2
Jackson, S.E.3
Main, E.R.G.4
Bracewell, D.G.5
Aeppli, G.6
Dalby, P.A.7
-
9
-
-
67849129183
-
High-throughput thermal scanning: A general, rapid dye-binding thermal shift screen for protein engineering
-
Lavinder, J. J., Hari, S. B., Sullivan, B. J. & Magliery, T. J. High-throughput thermal scanning: A general, rapid dye-binding thermal shift screen for protein engineering. J. Am. Chem. Soc. 131, 3794-3795 (2009).
-
(2009)
J. Am. Chem. Soc.
, vol.131
, pp. 3794-3795
-
-
Lavinder, J.J.1
Hari, S.B.2
Sullivan, B.J.3
Magliery, T.J.4
-
10
-
-
79960692993
-
Quantitation of protein-protein interactions by thermal stability shift analysis
-
Layton, C. J. & Hellinga, H. W. Quantitation of protein-protein interactions by thermal stability shift analysis. Protein Sci. 20, 1439-1450 (2011).
-
(2011)
Protein Sci.
, vol.20
, pp. 1439-1450
-
-
Layton, C.J.1
Hellinga, H.W.2
-
11
-
-
0023697408
-
Unfolding free energy changes determined by the linear extrapolation method. 1. Unfolding of phenylmethanesulfonyl alpha-chymotrypsin using different denaturants
-
Santoro, M. M. & Bolen, D. W. Unfolding free energy changes determined by the linear extrapolation method. 1. Unfolding of phenylmethanesulfonyl alpha-chymotrypsin using different denaturants. Biochemistry 27, 8063-8068 (1988).
-
(1988)
Biochemistry
, vol.27
, pp. 8063-8068
-
-
Santoro, M.M.1
Bolen, D.W.2
-
12
-
-
0030967237
-
Thermal denaturation of iso-l-cytochrome c variants: Comparison with solvent denaturation
-
Herrmann, L. M. & Bowler, B. E. Thermal denaturation of iso-1-cytochrome c variants: Comparison with solvent denaturation. Protein Sci. 6, 657-665 (1997). (Pubitemid 27120062)
-
(1997)
Protein Science
, vol.6
, Issue.3
, pp. 657-665
-
-
Herrmann, L.M.1
Bowler, B.E.2
-
13
-
-
79951577757
-
A high-throughput fluorescence chemical denaturation assay as a general screen for protein-ligand binding
-
Mahendrarajah, K., Dalby, P. A., Wilkinson, B., Jackson, S. E. & Main, E. R. G. A high-throughput fluorescence chemical denaturation assay as a general screen for protein-ligand binding. Anal. Biochem. 411, 155-157 (2011).
-
(2011)
Anal. Biochem.
, vol.411
, pp. 155-157
-
-
Mahendrarajah, K.1
Dalby, P.A.2
Wilkinson, B.3
Jackson, S.E.4
Main, E.R.G.5
-
14
-
-
16344382388
-
Thermodynamic stability of carbonic anhydrase: Measurements of binding affinity and stoichiometry using thermofluor
-
DOI 10.1021/bi048135v
-
Matulis, D., Kranz, J. K., Raymond Salemme, F. & Todd, M. J. Thermodynamic stability of carbonic anhydrase: Measurements of binding affinity and stoichiometry using ThermoFluor. Biochemistry 44, 5258-5266 (2005). (Pubitemid 40471238)
-
(2005)
Biochemistry
, vol.44
, Issue.13
, pp. 5258-5266
-
-
Matulis, D.1
Kranz, J.K.2
Salemme, F.R.3
Todd, M.J.4
-
15
-
-
0025234587
-
PH-Induced denaturation of proteins: A single salt bridge contributes 3-5 kcal/mol to the free energy of folding of T4 lysozyme
-
Anderson, D. E., Becktel, W. J. & Dahlquist, F. W. pH-Induced denaturation of proteins: a single salt bridge contributes 3-5 kcal/mol to the free energy of folding of T4 lysozyme. Biochemistry 29, 2403-2408 (1990). (Pubitemid 20095485)
-
(1990)
Biochemistry
, vol.29
, Issue.9
, pp. 2403-2408
-
-
Anderson, D.E.1
Becktel, W.J.2
Dahlquist, F.W.3
-
17
-
-
8344290520
-
Acid denaturation and refolding of green fluorescent protein
-
DOI 10.1021/bi048733+
-
Enoki, S., Saeki, K., Maki, K. & Kuwajima, K. Acid denaturation and refolding of green fluorescent protein. Biochemistry 43, 14238-14248 (2004). (Pubitemid 39482773)
-
(2004)
Biochemistry
, vol.43
, Issue.44
, pp. 14238-14248
-
-
Enoki, S.1
Saeki, K.2
Maki, K.3
Kuwajima, K.4
-
18
-
-
77952027734
-
Thermal trap for DNA replication
-
Mast, C. B. & Braun, D. Thermal trap for DNA replication. Phys. Rev. Lett. 104, 188102-188106 (2010).
-
(2010)
Phys. Rev. Lett.
, vol.104
, pp. 188102-188106
-
-
Mast, C.B.1
Braun, D.2
-
19
-
-
0642285283
-
Exponential DNA replication by laminar convection
-
Braun, D., Goddard, N. L. & Libchaber, A. Exponential DNA replication by laminar convection. Phys. Rev. Lett. 91, 158103-158106 (2003).
-
(2003)
Phys. Rev. Lett.
, vol.91
, pp. 158103-158106
-
-
Braun, D.1
Goddard, N.L.2
Libchaber, A.3
-
20
-
-
34848914313
-
Melting curve analysis in a shapshot
-
Baaske, P., Duhr, S. & Braun, D. Melting curve analysis in a shapshot. Appl. Phys. Lett. 91, 133901-133903 (2007).
-
(2007)
Appl. Phys. Lett.
, vol.91
, pp. 133901-133903
-
-
Baaske, P.1
Duhr, S.2
Braun, D.3
-
21
-
-
8344290520
-
Acid denaturation and refolding of green fluorescent protein
-
DOI 10.1021/bi048733+
-
Enoki, S., Saeki, K., Maki, K. & Kuwajima, K. Acid denaturation and refolding of green fluorescent protein. Biochemistry 43, 14238-14248 (2004) (Pubitemid 39482773)
-
(2004)
Biochemistry
, vol.43
, Issue.44
, pp. 14238-14248
-
-
Enoki, S.1
Saeki, K.2
Maki, K.3
Kuwajima, K.4
-
22
-
-
0036489443
-
Green Fluorescent Protein (GFP): Applications, structure, and related photophysical behavior
-
Zimmer, M. Green Fluorescent Protein (GFP): Applications, structure, and related photophysical behavior. Chem. Rev. 102, 759-782 (2002).
-
(2002)
Chem. Rev.
, vol.102
, pp. 759-782
-
-
Zimmer, M.1
-
23
-
-
0019892005
-
Renaturation of aequorea green-fluorescent protein
-
Bokman, S. H. & Ward, W. W. Renaturation of aequorea green-fluorescent protein. Biochem. Biophys. Res. Comm. 101, 1372-1380 (1981).
-
(1981)
Biochem. Biophys. Res. Comm.
, vol.101
, pp. 1372-1380
-
-
Bokman, S.H.1
Ward, W.W.2
-
24
-
-
1242264025
-
Thermal characteristics of recombinant green fluorescent protein (GFPuv) extracted from Escherichia coli
-
DOI 10.1111/j.1472-765X.2003.01460.x
-
Vessoni Penna, T. C., Ishii, M., Cholewa, O. & Souza, L. C. Thermal characteristics of recombinant green fluorescent protein (GFPuv) extracted from Escherichia coli. Letts. Appl. Microbiol. 38, 135-139 (2004). (Pubitemid 38232703)
-
(2004)
Letters in Applied Microbiology
, vol.38
, Issue.2
, pp. 135-139
-
-
Vessoni Penna, T.C.1
Ishii, M.2
Cholewa, O.3
De Souza, L.C.4
-
25
-
-
24044492592
-
Effect of pH on thermal- and chemical-induced denaturation of GFP
-
DOI 10.1385/ABAB:126:2:149
-
Alkaabi, K. M., Yafea, A. & Ashraf, S. S. Effect of pH on thermal-and chemical-induced denaturation of GFP. Appl. Biochem. Biotechnol. 126, 149-156 (2005). (Pubitemid 41213294)
-
(2005)
Applied Biochemistry and Biotechnology
, vol.126
, Issue.2
, pp. 149-156
-
-
Alkaabi, K.M.1
Yafea, A.2
Ashraf, S.S.3
-
26
-
-
33744981623
-
Image processing with ImageJ
-
Abramoff, M. D., Magalhaes, P. J. & Ram, S. J. Image processing with ImageJ. Biophotonics Intl. 11, 36-42 (2004).
-
(2004)
Biophotonics Intl.
, vol.11
, pp. 36-42
-
-
Abramoff, M.D.1
Magalhaes, P.J.2
Ram, S.J.3
-
27
-
-
0023442217
-
Protein stability curves
-
Becktel, W. J. & Schellman, J. A. Protein stability curves. Biopolymers 26, 1859-1877 (1987).
-
(1987)
Biopolymers
, vol.26
, pp. 1859-1877
-
-
Becktel, W.J.1
Schellman, J.A.2
-
28
-
-
0030029469
-
Interactions of the CelS binding ligand with various receptor domains of the Clostridium thermocellum cellulosomal scaffolding protein, CipA
-
Lytle, B., Myers, C., Kruus, K. & Wu, J. H. Interactions of the CelS binding ligand with various receptor domains of the Clostridium thermocellum cellulosomal scaffolding protein, CipA. J. Bacteriol. 178, 1200-1203 (1996). (Pubitemid 26048041)
-
(1996)
Journal of Bacteriology
, vol.178
, Issue.4
, pp. 1200-1203
-
-
Lytle, B.1
Myers, C.2
Kruus, K.3
Wu, J.H.D.4
-
29
-
-
34848914313
-
Melting curve analysis in a snapshot
-
Baaske, P., Duhr, S. & Braun, D. Melting curve analysis in a snapshot. Appl. Phys. Lett. 91, 133901 (2007).
-
(2007)
Appl. Phys. Lett.
, vol.91
, pp. 133901
-
-
Baaske, P.1
Duhr, S.2
Braun, D.3
-
30
-
-
10844287980
-
Thermophoresis of DNA determined by microfluidic fluorescence
-
DOI 10.1140/epje/i2004-10073-5
-
Duhr, S., Arduini, S. & Braun, D. Thermophoresis and DNA determined by microfluidic fluorescence. Eur. Phys. J. E. 15, 277-286 (2004). (Pubitemid 40003073)
-
(2004)
European Physical Journal E
, vol.15
, Issue.3
, pp. 277-286
-
-
Duhr, S.1
Arduini, S.2
Braun, D.3
-
31
-
-
0033542301
-
Incompressible limit for solutions of the isentropic Navier-Stokes equations with Dirichlet boundary conditions
-
Desjardins, B., Grenier, E., Lions, P. L. & Masmoudi, N. Incompressible limit for solutions of the isentropic Navier-Stokes equations with Dirichlet boundary conditions. J. Math. Pur. Appl. 78, 461-471 (1998).
-
(1998)
J. Math. Pur. Appl.
, vol.78
, pp. 461-471
-
-
Desjardins, B.1
Grenier, E.2
Lions, P.L.3
Masmoudi, N.4
|