A high-throughput fluorescence chemical denaturation assay as a general screen for protein-ligand binding

Analytical Biochemistry

Volumn 411, Issue 1, 2011, Pages 155-157

  Mahendrarajah, Kumaran ^a   Dalby, Paul A ^b   Wilkinson, Barrie ^c   Jackson, Sophie E ^a   Main, Ewan R G ^d  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^b UNIVERSITY COLLEGE LONDON   (United Kingdom)

^c BIOTICA TECHNOLOGY LTD   (United Kingdom)

^d QUEEN MARY UNIVERSITY OF LONDON   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

BINDING ENERGY; COMPLEXATION; DENATURATION; FLUORESCENCE; KETONES; PROTEINS;

BINDING AFFINITIES; CHEMICAL DENATURATION; HIGH THROUGHPUT; INTRINSIC FLUORESCENCE; LIGAND COMPLEXES; LIGAND-PROTEIN COMPLEXES; POTENTIAL LIGAND; WIDE DYNAMIC RANGE;

LIGANDS;

IMMUNOPHILIN; LIGAND; PROTEIN FKBP12; RAPAMYCIN; UNCLASSIFIED DRUG;

ARTICLE; DENATURATION; FLUORESCENCE; HIGH THROUGHPUT SCREENING; LIGAND BINDING; PRIORITY JOURNAL; PROTEIN BINDING;

EID: 79951577757     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2010.12.001     Document Type: Article

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