Sulfatase-activated fluorophores for rapid discrimination of mycobacterial species and strains

Proceedings of the National Academy of Sciences of the United States of America

Volumn 110, Issue 32, 2013, Pages 12911-12916

  Beatty, Kimberly E ^a,d   Williams, Monique ^b,c   Carlson, Brian L ^a   Swarts, Benjamin M ^a   Warren, Robin M ^b   Van Helden, Paul D ^b   Bertozzi, Carolyn R ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF STELLENBOSCH   (South Africa)

^c UNIVERSITY OF CAPE TOWN   (South Africa)

^d OREGON HEALTH AND SCIENCE UNIVERSITY   (United States)

Author keywords

Chemical biology; Enzyme assay; Fluorescence; Hydrolase; Mycobacterium tuberculosis

Indexed keywords

7 HYDROXY 9H (1,3 DICHLOROACTION 9,9 DIMETHYLACRIDIN 2 ONE)SULFATE; ARYLSULFATASE; ARYLSULFATASE D; ARYLSULFATASE F; ARYLSULFATASE G; CEREBROSIDE SULFATASE; CYSTEINE; ESTERASE; HYDROLASE; HYMECROMONE; N ACETYLGALACTOSAMINE 4 SULFATASE; NUCLEOPHILE; PHOSPHATASE; SULFATASE; SULFATE; UNCLASSIFIED DRUG; ZINC;

ARTICLE; BIOINFORMATICS; CELL LYSATE; COLORIMETRY; CONSENSUS SEQUENCE; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME MECHANISM; GEL ELECTROPHORESIS; GENETIC CONSERVATION; HUMAN; HYDROLYSIS; IN VITRO STUDY; LIMIT OF DETECTION; MASS SPECTROMETRY; METAL BINDING; MICROBIAL IDENTIFICATION; MOLECULAR PROBE; MOLECULAR WEIGHT; MYCOBACTERIUM; MYCOBACTERIUM AFRICANUM; MYCOBACTERIUM AVIUM; MYCOBACTERIUM BOVIS; MYCOBACTERIUM CANETTI; MYCOBACTERIUM INTRACELLULARE; MYCOBACTERIUM KANSASII; MYCOBACTERIUM MARINUM; MYCOBACTERIUM NONCHROMOGENICUM; MYCOBACTERIUM SCROFULACEUM; MYCOBACTERIUM SEPTICUM; MYCOBACTERIUM SMEGMATIS; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PROTEIN STRUCTURE; QUANTUM YIELD; SULFATION; ULTRAVIOLET RADIATION;

CHEMICAL BIOLOGY; ENZYME ASSAY; FLUORESCENCE; HYDROLASE; MYCOBACTERIUM TUBERCULOSIS;

ACRIDONES; ANIMALS; BACTERIAL PROTEINS; CELL LINE; CELL LINE, TUMOR; CERCOPITHECUS AETHIOPS; CHO CELLS; COS CELLS; CRICETINAE; CRICETULUS; ELECTROPHORESIS; FLUORESCENT DYES; GELS; HEK293 CELLS; HUMANS; JURKAT CELLS; MOLECULAR STRUCTURE; MYCOBACTERIUM; MYCOBACTERIUM TUBERCULOSIS; SPECIES SPECIFICITY; SUBSTRATE SPECIFICITY; SULFATASES; SULFATES; SULFURIC ACID ESTERS; TUBERCULOSIS;

EID: 84881465669     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1222041110     Document Type: Article

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