Quantification of β-galactosidase activity after non-viral transfection in vivo

Journal of Controlled Release

Volumn 91, Issue 1-2, 2003, Pages 201-208

  Zinselmeyer, Bernd H ^a,b   Beggbie, Nicola ^a   Uchegbu, Ijeoma F ^b   Schätzlein, Andreas G ^a  

^a UNIVERSITY OF GLASGOW   (United Kingdom)

^b UNIVERSITY OF STRATHCLYDE   (United Kingdom)

Author keywords

Fluorescence assay; Gene expression; In vivo; Reporter gene; Galactosidase

Indexed keywords

BIOASSAY; DEGRADATION; ENZYMES; FLUORESCENCE; GENES; HEMOGLOBIN; REACTION KINETICS; TISSUE;

GENE DELIVERY SYSTEMS;

CONTROLLED DRUG DELIVERY;

7 HYDROXY 9H (1,3 DICHLORO 9,9 DIMETHYLACRIDIN 2 ONE); ACRIDINE DERIVATIVE; BETA GALACTOSIDASE; CARBOHYDRATE DERIVATIVE; GALACTOPYRANOSIDE; HEMOGLOBIN; POLYETHYLENEIMINE; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; ANIMAL TISSUE; CONFERENCE PAPER; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ASSAY; FLUORESCENCE; GENE ACTIVITY; GENETIC TRANSFECTION; IN VIVO STUDY; NONHUMAN; NONVIRAL GENE DELIVERY SYSTEM; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; SPECTROFLUOROMETRY; TRANSGENE; ANIMAL; ARTICLE; BIOSYNTHESIS; CHEMISTRY; ENZYMOLOGY; ERYTHROCYTE; ESCHERICHIA COLI; GENETICS; LACTOSE OPERON; LIVER; LUNG; MOUSE; PLASMID; REPORTER GENE; TRANSGENIC MOUSE;

ANIMALS; BETA-GALACTOSIDASE; ERYTHROCYTES; ESCHERICHIA COLI; GENES, REPORTER; HEMOGLOBINS; LAC OPERON; LIVER; LUNG; MICE; MICE, TRANSGENIC; PLASMIDS; TRANSFECTION;

EID: 0141886980     PISSN: 01683659     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-3659(03)00234-7     Document Type: Conference Paper

References (28)

1. Torbett B.E. Reporter genes: too much of a good thing? J. Gene Med. 4:(5):2002;478-479.
2. Young S.L., Jackson A.E., Puett D., Melner M.H. Detection of chloramphenicol acetyl transferase activity in transfected cells: a rapid and sensitive HPLC-based method. DNA. 4:(6):1985;469-475.
3. Sleigh M.J. A nonchromatographic assay for expression of the chloramphenicol acetyltransferase gene in eucaryotic cells. Anal. Biochem. 156:(1):1986;251-256.
4. Nielsen D.A., Chang T.C., Shapiro D.J. A highly sensitive, mixed-phase assay for chloramphenicol acetyltransferase activity in transfected cells. Anal. Biochem. 179:(1):1989;19-23.
5. De Maio A., Buchman T.G. Mammalian liver contains an activity which mimics bacterial chloramphenicol acetyltransferase. Biochim. Biophys. Acta. 1087:(3):1990;303-308.
6. Hoffman R. Green fluorescent protein imaging of tumour growth, metastasis, and angiogenesis in mouse models. Lancet Oncol. 3:(9):2002;546-556.
7. Coralli C., Cemazar M., Kanthou C., Tozer G.M., Dachs G.U. Limitations of the reporter green fluorescent protein under simulated tumor conditions. Cancer Res. 61:(12):2001;4784-4790.
8. Tseng W.C., Haselton F.R., Giorgio T.D. Transfection by cationic liposomes using simultaneous single cell measurements of plasmid delivery and transgene expression. J. Biol. Chem. 272:(41):1997;25641-25647.
9. B. Guo, A.G. Pearce, K.E. Traulsen, A.C. Rintala, H. Lee, Fluorescence produced by transfection reagents can be confused with green fluorescent proteins in mammalian cells, Biotechniques 31(2) (2001) 314-316, 318, 320-321.
10. Schenborn E., Groskreutz D. Reporter gene vectors and assays. Mol. Biotechnol. 13:(1):1999;29-44.
11. Colin M., Moritz S., Schneider H., Capeau J., Coutelle C., Brahimi-Horn M.C. Haemoglobin interferes with the ex vivo luciferase luminescence assay: consequence for detection of luciferase reporter gene expression in vivo. Gene Ther. 7:(15):2000;1333-1336.
12. Ausubel F., Brent R., Kingston E., Moore D., Seidman J., Smith J., Struhl K. Current Protocols in Molecular Biology. Vols. I-III:1998;Wiley, New York.
13. Weiss D.J., Liggitt D., Clark J.G. Histochemical discrimination of endogenous mammalian beta-galactosidase activity from that resulting from lac-Z gene expression. Histochem. J. 31:(4):1999;231-236.
14. Fiering S.N., Roederer M., Nolan G.P., Micklem D.R., Parks D.R., Herzenberg L.A. Improved facs-gal-flow cytometric analysis and sorting of viable eukaryotic cells expressing reporter gene constructs. Cytometry. 12:(4):1991;291-301.
15. Olesen C.E., Fortin J.J., Voyta J.C., Bronstein I. Detection of beta-galactosidase and beta-glucuronidase using chemiluminescent reporter gene assays. Methods Mol. Biol. 63:1997;61-70.
16. Arakawa H., Tsuji A., Maeda M. Chemiluminescent assay of beta-D-galactosidase based on indole luminescence. J. Biolumin. Chemilumin. 13:(6):1998;349-354.
17. Bronstein I., Martin C.S., Fortin J.J., Olesen C.E., Voyta J.C. Chemiluminescence: sensitive detection technology for reporter gene assays. Clin. Chem. 42:(9):1996;1542-1546.
18. D.A. Nazarenko, S.D. Dertinger, T.A. Gasiewicz, Enhanced detection of beta-galactosidase reporter activation is achieved by a reduction of hemoglobin content in tissue lysates, Biotechniques 30(4) (2001) 776-777, 780-781.
19. Pelisek J., Armeanu S., Nikol S. Evaluation of beta-galactosidase activity in tissue in the presence of blood. J. Vasc. Res. 37:(6):2000;585-593.
20. Smith A.D., Trempe J.P. Luminometric quantitation of photinus pyralis firefly luciferase and Escherichia coli beta-galactosidase in blood-contaminated organ lysates. Anal. Biochem. 286:(1):2000;164-172.
21. van der Loo B., Fenton M.J., Erusalimsky J.D. Cytochemical detection of a senescence-associated beta-galactosidase in endothelial and smooth muscle cells from human and rabbit blood vessels. Exp. Cell Res. 241:(2):1998;309-315.
22. Krishna D.R., Sperker B., Fritz P., Klotz U. Does pH 6 beta-galactosidase activity indicate cell senescence? Mech. Ageing Dev. 109:(2):1999;113-123.
23. Coates P.J., Lorimore S.A., Rigat B.A., Lane D.P., Wright E.G. Induction of endogenous beta-galactosidase by ionizing radiation complicates the analysis of p53-LacZ transgenic mice. Oncogene. 20:(48):2001;7096-7097.
24. Goula D., Benoist C., Mantero S., Merlo G., Levi G., Demeneix B.A. Polyethylenimine-based intravenous delivery of transgenes to mouse lung. Gene Ther. 5:(9):1998;1291-1295.
25. Bragonzi A., Boletta A., Biffi A., Muggia A., Sersale G., Cheng S.H., Bordignon C., Assael B.M., Conese M. Comparison between cationic polymers and lipids in mediating systemic gene delivery to the lungs. Gene Ther. 6:(12):1999;1995-2004.
26. Coll J.L., Chollet P., Brambilla E., Desplanques D., Behr J.P., Favrot M. In vivo delivery to tumors of DNA complexed with linear polyethylenimine. Hum. Gene Ther. 10:(10):1999;1659-1666.
27. Goula D., Becker N., Lemkine G.F., Normandie P., Rodrigues J., Mantero S., Levi G., Demeneix B.A. Rapid crossing of the pulmonary endothelial barrier by polyethylenimine/DNA complexes. Gene Ther. 7:(6):2000;499-504.
28. Li S., Tan Y., Viroonchatapan E., Pitt B.R., Huang L. Targeted gene delivery to pulmonary endothelium by anti-PECAM antibody. Am. J. Physiol. 278:(3):2000;L504-L511.