Highly efficient SiRNA and gene transfer into hepatocyte-like HepaRG cells and primary human hepatocytes: New means for drug metabolism and toxicity studies

Methods in Molecular Biology

Volumn 987, Issue , 2013, Pages 295-314

  Laurent, Véronique ^a,b   Glaise, Denise ^c   Nübel, Tobias ^d   Gilot, David ^e   Corlu, Anne ^c   Loyer, Pascal ^b,c  

^a BREST UNIVERSITY HOSPITAL   (France)

^b SynNanoVect Platform   (France)

^c Univ Rennes   (France)

^d Lonza Cologne GmbH   (Germany)

^e UNIVERSITÉ DE RENNES 1   (France)

Author keywords

Drug metabolism; HepaRG cells; Human hepatocytes; In vitro toxicity; Nucleofection ; Transfection

Indexed keywords

DIMETHYL SULFOXIDE; GREEN FLUORESCENT PROTEIN; LIPID; LIPOSOME; PLASMID DNA; POLYMER; SMALL INTERFERING RNA; DRUG; DYES, REAGENTS, INDICATORS, MARKERS AND BUFFERS;

ANIMAL CELL; ARTICLE; CELL DIFFERENTIATION; CELL LINE; CELL PROLIFERATION; DNA TRANSFER; ELECTROPORATION; EXPRESSION VECTOR; GENE FUNCTION; HEPARG CELL; HUMAN; HUMAN CELL; LIVER CELL; NONHUMAN; NONVIRAL GENE DELIVERY SYSTEM; PRIORITY JOURNAL; RNA TRANSPORT; STEM CELL; STEM CELL CULTURE; STEM CELL EXPANSION; TRANSIENT TRANSFECTION; CHEMISTRY; CULTURE TECHNIQUE; CYTOLOGY; DRUG TOXICITY; GENETIC TRANSFECTION; GENETICS; METABOLISM; METHODOLOGY;

CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CELL PROLIFERATION; DRUG TOXICITY; ELECTROPORATION; HEPATOCYTES; HUMANS; INDICATORS AND REAGENTS; PHARMACEUTICAL PREPARATIONS; RNA, SMALL INTERFERING; STEM CELLS; TRANSFECTION;

EID: 84881308001     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-321-3_25     Document Type: Article

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