Downscaling the Analysis of Complex Transmembrane Signaling Cascades to Closed Attoliter Volumes

PLoS ONE

Volumn 8, Issue 8, 2013, Pages

  Grasso, Luigino ^a   Wyss, Romain ^a   Piguet, Joachim ^a   Werner, Michael ^a   Hassaïne, Ghérici ^a   Hovius, Ruud ^a   Vogel, Horst ^a  

^a EPFL   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

CELL SURFACE RECEPTOR; G PROTEIN COUPLED RECEPTOR; RETINA S ANTIGEN;

ARTICLE; CELL ACTIVATION; CELL FUNCTION; CELL INTERACTION; CELL MEMBRANE; CELL STIMULATION; COMPLEX FORMATION; CONTROLLED STUDY; CYTOSKELETON; CYTOSOL; FLUORESCENCE MICROSCOPY; LIGAND BINDING; MAMMAL CELL; OPTICAL TWEEZERS; RECEPTOR BINDING; SIGNAL DETECTION; SIGNAL TRANSDUCTION; SINGLE CELL ANALYSIS; STIMULUS RESPONSE;

EID: 84881112332     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0070929     Document Type: Article

References (38)

1. Martins SAM, Trabuco JRC, Monteiro GA, Chu V, Conde JP, et al. (2012) Towards the miniaturization of GPCR-based live-cell screening assays. Trends Biotechnol 30: 566-574.
2. Neuži P, Giselbrecht S, Länge K, Huang TJ, Manz A, (2012) Revisiting lab-on-a-chip technology for drug discovery. Nat Rev Drug Discov 11: 620-632.
3. Pick H, Schmid E, Tairi A-P, Ilegems E, Hovius R, et al. (2005) Investigating cellular signaling reactions in single attoliter vesicles. J Am Chem Soc 127: 2908-2912.
4. Gorris HH, Walt DR, (2010) Analytical Chemistry on the Femtoliter Scale. Angew Chem Int Ed Engl 49: 3880-3895.
5. Christensen SM, Stamou DG, (2010) Sensing-applications of surface-based single vesicle arrays. Sensors (Basel) 10: 11352-11368.
6. Stamou DG, Duschl C, Delamarche E, Vogel H, (2003) Self-assembled microarrays of attoliter molecular vessels. Angew Chem Int Ed Engl 42: 5580-5583.
7. Merenda F, Rohner J, Lamothe E, Pascoal P, Fournier J-M, et al. (2007) Micro-optics for optical trapping in microfluidics. Proc SPIE 6644: P-1-66440P-10.
8. Dzieciol AJ, Mann S, (2011) Designs for life: protocell models in the laboratory. Chem Soc Rev 41: 79-85.
9. Chan Y-HM, Boxer SG, (2007) Model membrane systems and their applications. Curr Opin Chem Biol 11: 581-587.
10. Bally M, Bailey K, Sugihara K, Grieshaber D, Voeroes J, et al. (2010) Liposome and Lipid Bilayer Arrays Towards Biosensing Applications. Small 6: 2481-2497.
11. Staedler B, Price AD, Chandrawati R, Hosta-Rigau L, Zelikin AN, et al. (2009) Polymer hydrogel capsules: en route toward synthetic cellular systems. Nanoscale 1: 68-73.
12. Bolinger P-Y, Stamou DG, Vogel H, (2008) An integrated self-assembled nanofluidic system for controlled biological chemistries. Angew Chem Int Ed Engl 47: 5544-5549.
13. Scott RE, (1976) Plasma membrane vesiculation: a new technique for isolation of plasma membranes. Science 194: 743-745.
14. Sezgin E, Kaiser H-J, Baumgart T, Schwille P, Simons K, et al. (2012) Elucidating membrane structure and protein behavior using giant plasma membrane vesicles. Nat Protoc 7: 1042-1051.
15. Pascoal P, Kosanic D, Gjoni M, Vogel H, (2010) Membrane nanotubes drawn by optical tweezers transmit electrical signals between mammalian cells over long distances. Lab Chip 10: 2235-2241.
16. Baumgart T, Hammond AT, Sengupta P, Hess ST, Holowka DA, et al. (2007) Large-scale fluid/fluid phase separation of proteins and lipids in giant plasma membrane vesicles. Proc Natl Acad Sci U S A 104: 3165-3170.
17. Lingwood D, Ries J, Schwille P, Simons K, (2008) Plasma membranes are poised for activation of raft phase coalescence at physiological temperature. Proc Natl Acad Sci U S A 105: 10005-10010.
18. Levental I, Grzybek M, Simons K, (2011) Raft domains of variable properties and compositions in plasma membrane vesicles. Proc Natl Acad Sci U S A 108: 11411-11416.
19. Bauer B, Davidson M, Orwar O, (2009) Proteomic analysis of plasma membrane vesicles. Angew Chem Int Ed Engl 48: 1656-1659.
20. Rosenbaum DM, Rasmussen SGF, Kobilka BK, (2009) The structure and function of G-protein-coupled receptors. Nature 459: 356-363.
21. Doherty GJ, Mcmahon HT, (2008) Mediation, modulation, and consequences of membrane-cytoskeleton interactions. Annu Rev Biophys 37: 65-95.
22. Hall D, (2008) Analysis and interpretation of two-dimensional single-particle tracking microscopy measurements: Effect of local surface roughness. Anal Biochem 377: 24-32.
23. Yu N, Atienza JM, Bernard J, Blanc S, Zhu J, et al. (2006) Real-time monitoring of morphological changes in living cells by electronic cell sensor arrays: an approach to study G protein-coupled receptors. Anal Chem 78: 35-43.
24. Roizard S, Danelon C, Hassaine G, Piguett J, Schulze K, et al. (2011) Activation of G-Protein-Coupled Receptors in Cell-Derived Plasma Membranes Supported on Porous Beads. J Am Chem Soc 133: 16868-16874.
25. Hein P, Frank M, Hoffmann C, Lohse MJ, Bünemann M, (2005) Dynamics of receptor/G protein coupling in living cells. EMBO J 24: 4106-4114.
26. Jean-Alphonse F, Hanyaloglu AC, (2011) Regulation of GPCR signal networks via membrane trafficking. Mol Cell Endocrinol 331: 205-214.
27. Schmidlin F, Déry O, Bunnett NW, Grady EF, (2002) Heterologous regulation of trafficking and signaling of G protein-coupled receptors: beta-arrestin-dependent interactions between neurokinin receptors. Proc Natl Acad Sci U S A 99: 3324-3329.
28. Cherney DP, Bridges TE, Harris JM, (2004) Optical trapping of unilamellar phospholipid vesicles: investigation of the effect of optical forces on the lipid membrane shape by confocal-Raman microscopy. Anal Chem 76: 4920-4928.
29. Johnsen S, Widder E, (1999) The physical basis of transparency in biological tissue: ultrastructure and the minimization of light scattering. J Theor Biol 199: 181-198.
30. Moerner WE, (2009) Single-Molecule Optical Spectroscopy and Imaging: From Early Steps to Recent Advances. In: Gräslund A, Rigler R, Widengren J, editors. Single Molecule Spectroscopy in Chemistry, Physics and Biology: Nobel Symposium (Springer Series in Chemical Physics). Berlin, Heidelberg: Springer. Vol. 96: 25-60.
31. Hell SW, (2009) Far-Field Optical Nanoscopy. In: Gräslund A, Rigler R, Widengren J, editors. Single Molecule Spectroscopy in Chemistry, Physics and Biology: Nobel Symposium (Springer Series in Chemical Physics). Berlin, Heidelberg: Springer. Vol. 96: 365-398.
32. Altschuler SJ, Wu LF, (2010) Cellular heterogeneity: do differences make a difference? Cell 141: 559-563.
33. Wistuba II, Gelovani JG, Jacoby JJ, Davis SE, Herbst RS, (2011) Methodological and practical challenges for personalized cancer therapies. Nat Rev Clin Oncol 8: 135-141.
34. De Souza N, (2012) Single-cell methods. Nat Methods 9: 35.
35. Ruiz-Velasco V, Ikeda SR, (2001) Functional expression and FRET analysis of green fluorescent proteins fused to G-protein subunits in rat sympathetic neurons. J Physiol 537: 679-692.
36. Barak L, Ferguson S, Zhang J, Caron MG, (1997) A beta-arrestin green fluorescent protein biosensor for detecting G protein-coupled receptor activation. J Biol Chem 272: 27497-27500.
37. Koopman WJH, Distelmaier F, Hink MA, Verkaart S, Wijers M, et al. (2008) Inherited complex I deficiency is associated with faster protein diffusion in the matrix of moving mitochondria. Am J Physiol Cell Physiol 294: C1124-C1132.
38. PicoQuant GmbH website. Available: http://www.picoquant.com/technotes/appnote_diffusion_coefficients.pdf. Accessed 2013 July 3.