Dynamics of receptor/G protein coupling in living cells

EMBO Journal

Volumn 24, Issue 23, 2005, Pages 4106-4114

  Hein, Peter ^a   Frank, Monika ^a   Hoffmann, Carsten ^a   Lohse, Martin J ^a   Bünemann, Moritz ^a  

^a UNIVERSITY OF WÜRZBURG   (Germany)

Author keywords

2A; FRET; G protein; GIRK; Kinetics; Receptors

Indexed keywords

ALPHA 2 ADRENERGIC RECEPTOR; GUANINE NUCLEOTIDE BINDING PROTEIN; INWARDLY RECTIFYING POTASSIUM CHANNEL; MUTANT PROTEIN; POTASSIUM ION;

ARTICLE; CONTROLLED STUDY; DISSOCIATION; EMBRYO; FLUORESCENCE RESONANCE ENERGY TRANSFER; HUMAN; HUMAN CELL; ION CURRENT; MOLECULAR DYNAMICS; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PROTEIN INTERACTION;

BACTERIAL PROTEINS; CELL LINE; CELL MEMBRANE; FLUORESCENCE RESONANCE ENERGY TRANSFER; FLUORESCENT DYES; GTP-BINDING PROTEINS; HUMANS; KINETICS; LUMINESCENT PROTEINS; RECEPTORS, ADRENERGIC, ALPHA-2; RECEPTORS, G-PROTEIN-COUPLED; STAINING AND LABELING;

EID: 28644443374     PISSN: 02614189     EISSN: 14602075     Source Type: Journal    
DOI: 10.1038/sj.emboj.7600870     Document Type: Article

References (37)

1. Azpiazu I, Gautam N (2004) A FRET based sensor indicates that receptor access to a G protein is unrestricted in a living mammalian cell. J Biol Chem 279: 27709-27718
2. Bourne HR (1997) How receptors talk to trimeric G proteins. Curr Opin Cell Biol 9: 134-142
3. + current in cultured guinea-pig atrial myocytes. J Physiol 501: 549-554
4. + channel currents. J Biol Chem 276: 47512-47517
5. Bünemann M, Frank M, Lohse MJ (2003) Gi protein activation in intact cells involves subunit rearrangement rather than dissociation. Proc Natl Acad Sci USA 100: 16077-16082
6. Chidiac P (1998) Rethinking receptor-G protein-effector interactions. Biochem Pharmacol 55: 549-556
7. iα1 and the mechanism of GTP hydrolysis. Science 265: 1405-1412
8. 3H]rauwolscine? A comparison of nine tissues and cell lines. J Pharmacol Exp Ther 273: 1287-1294
9. Förster T (1948) Zwischenmolekulare Energiewanderung und Fluoreszenz. Ann Phys (Leipzig) 2: 55-75
10. αi- specific region. J Biol Chem 280: 24584-24590
11. Gales C, Rebois RV, Hogue M, Trieu P, Breit A, Hebert TE, Bouvier M (2005) Real-time monitoring of receptor and G-protein interactions in living cells. Nat Methods 2: 177-184
12. 2 adenosine receptors. II. A restricted collision-coupling model of receptor-effector interaction. Mol Pharmacol 39: 524-530
13. Hakak Y, Shrestha D, Goegel MC, Behan DP, Chalmers DT (2003) Global analysis of G-protein-coupled receptor signaling in human tissues. FEBS Lett 550: 11-17
14. Hamm HE (1998) The many faces of G protein signaling. J Biol Chem 273: 669-672
15. Heck M, Hofmann KP (2001) Maximal rate and nucleotide dependence of rhodopsin-catalyzed transducin activation: initial rate analysis based on a double displacement mechanism. J Biol Chem 276: 10000-10009
16. Herrmann R, Heck M, Henklein P, Kleuss C, Hofmann KP, Ernst OP (2004) Sequence of interactions in receptor-G protein coupling. J Biol Chem 279: 24283-24290
17. Hoffmann C, Gaietta G, Bünemann M, Adams SR, Oberdorff-Maass S, Behr B, Vilardaga JP, Tsien RY, Ellisman MH, Lohse MJ (2005) A FlAsH-based FRET approach to determine G protein-coupled receptor activation in living cells. Nat Methods 2: 171-176
18. + (GIRK) channels by Gβγ subunits. J Biol Chem 278: 1037-1043
19. Huang C, Hepler JR, Chen LT, Gilman AG, Anderson RG, Mumby SM (1997) Organization of G proteins and adenylyl cyclase at the plasma membrane. Mol Biol Cell 8: 2365-2378
20. Hur EM, Kim KT (2002) G protein-coupled receptor signalling and cross-talk: achieving rapidity and specificity. Cell Signal 14: 397-405
21. Janetopoulos C, Jin T, Devreotes P (2001) Receptor-mediated activation of heterotrimeric G-proteins in living cells. Science 291: 2408-2411
22. 2-adrenergic receptor requires both receptor phosphorylation and receptor activation. J Biol Chem 280: 9528-9535
23. Miyawaki A (2003) Visualization of the spatial and temporal dynamics of intracellular signaling. Dev Cell 4: 295-305
24. Nagai T, Ibata K, Park ES, Kubota M, Mikoshiba K, Miyawaki A (2002) A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat Biotechnol 20: 87-90
25. Neer EJ (1995) Heterotrimeric G proteins: organizers of transmembrane signals. Cell 80: 249-257
26. Neubig RR (1994) Membrane organization in G-protein mechanisms. FASEB J 8: 939-946
27. 2 adrenergic receptors: evidence for a precoupled receptor-guanine nucleotide protein complex. Biochemistry 27: 2374-2384
28. Orly J, Schramm M (1976) Coupling of catecholamine receptor from one cell with adenylate cyclase from another cell by cell fusion. Pmc Natl Acad Sci USA 73: 4410-4414
29. Ruiz-Velasco V, Ikeda SR (2001) Functional expression and FRET analysis of green fluorescent proteins fused to G-protein subunits in rat sympathetic neurons. J Physiol 537: 679-692
30. Seifert R, Wenzel-Seifert K (2002) Constitutive activity of G-protein-coupled receptors: cause of disease and common property of wild-type receptors. Naunyn Schmiedebergs Arch Pharmacol 366: 381-416
31. Tolkovsky AM, Levitzki A (1978) Mode of coupling between the β-adrenergic receptor and adenylate cyclase in turkey erythrocytes. Biochemistry 17: 3795
32. Tsien RY (1998) The green fluorescent protein. Annu Rev Biochem 67: 509-544
33. Vilardaga JP, Bünemann M, Krasel C, Castro M, Lohse MJ (2003) Measurement of the millisecond activation switch of G protein-coupled receptors in living cells. Nat Biotechnol 21: 807-812
34. i1 α subunit abolishes its endogenous GTPase activity. Biochem J 379: 673-679
35. Wickman KD, Iniguez-Lluhl JA, Davenport PA, Taussig R, Krapivinsky GB, Linder ME, Gilman AG, Clapham DE (1994) Recombinant G-protein βγ-subunits activate the muscarinic-gated atrial potassium channel. Nature 368: 255-257
36. Wu YL, Hooks S, Harden TK, Dohlman HG (2004) Dominant-negative inhibition of pheromone receptor signaling by a single point mutation in the G protein alpha subunit. J Biol Chem 279: 35287-35297
37. Yi TM, Kitano H, Simon MI (2003) A quantitative characterization of the yeast heterotrimeric G protein cycle. Proc Natl Acad Sci USA 100: 10764-10769