Antineoplastic effects of deoxyelephantopin, a sesquiterpene lactone from Elephantopus scaber, on lung adenocarcinoma (A549) cells

Journal of Chinese Integrative Medicine

Volumn 11, Issue 4, 2013, Pages 269-277

  Kabeer, Farha A ^a   Sreedevi, Geetha B ^b   Nair, Mangalam S ^c   Rajalekshmi, Dhanya S ^c   Gopalakrishnan, Latha P ^b   Kunjuraman, Sujathan ^a   Prathapan, Remani ^a  

^a REGIONAL CANCER CENTRE   (India)

^b Trop Botanic Gdn and Res Inst   (India)

^c REGIONAL RESEARCH LABORATORY CSIR   (India)

Author keywords

A549 cells; Apoptosis; Caspases; Cell line; Deoxyelephantopin; Elephantopus scaber; Herbal medicine; Lung neoplasms; Tumor

Indexed keywords

CASPASE 2; CASPASE 3; CASPASE 8; CASPASE 9; DEOXYELEPHANTOPIN; SESQUITERPENE LACTONE; UNCLASSIFIED DRUG;

ANTINEOPLASTIC ACTIVITY; APOPTOSIS; ARTICLE; CELL CYCLE; CELL DEATH; CELL STRUCTURE; COLONY FORMATION; CONTROLLED STUDY; CYTOTOXICITY; DNA CONTENT; DNA FRAGMENTATION; ELEPHANTOPUS SCABER; ENZYME ACTIVITY; FLOW CYTOMETRY; HUMAN; HUMAN CELL; LUNG ADENOCARCINOMA; NICK END LABELING; WESTERN BLOTTING;

EID: 84881021704     PISSN: 16721977     EISSN: None     Source Type: Journal    
DOI: 10.3736/jintegrmed2013040     Document Type: Article

References (30)

1. Nuñez G, Benedict MA, Hu Y, Inohara N. Caspases: the proteases of the apoptotic pathway. Oncogene. 1998; 17(25): 3237-3245.
2. Kaufmann SH, Earnshaw WC. Induction of apoptosis by cancer chemotherapy. Exp Cell Res. 2000; 256(1): 42-49.
3. Hammer ML, Johns EA. Tapping an Amazônian plethora: four medicinal plants of Marajó Island, Pará (Brazil). J Ethanopharmocol. 1993; 40(1): 53-75.
4. Sankar V, Kalirajan R, Sweetlin Vivian Sales F, Raghuraman S. Antiinflammatory activity of Elephantopus scaber in albino rats. Indian J Pharm Sci. 2001; 63(6): 523-525.
5. Daisy P, Priya CE. Hypolipidemic and renal functionality potentials of the hexane extract fractions of Elephantopus scaber Linn. Int J Biomed Sci. 2010; 6(3): 241-245.
6. Rajesh MG, Latha MS. Hepatoprotection by Elephantopus scaber Linn. in CCl4-induced liver injury. Indian J Physiol Pharmacol. 2001; 45(4): 481-486.
7. Daisy P, Jasmine R, Ignacimuthu S, Murugan E. A novel steroid from Elephantopus scaber L. an ethanomedicinal plant with antidiabetic activity. Phytomedicine. 2009; 16(2-3): 252-257.
8. Geetha BS, Latha PG, Remani P. Evaluation of Elephantopus scaber on the inhibition of chemical carcinogenesis and tumor development in mice. Pharm Biol. 2010; 48(3): 342-348.
9. Singh SDJ, Krishna V, Mankani KL, Manjunatha BK, Vidya SM, Manohara YN. Wound healing activity of the leaf extracts and deoxyelephantopin isolated from Elephantopus scaber Linn. Indian J Pharmacol. 2005; 37(4): 238-242.
10. Geetha BS, Nair MS, Latha PG, Remani P. Sesquiterpene lactones isolated from Elephantopus scaber L. inhibits human lymphocyte proliferation and the growth of tumour cell lines and induces apoptosis in vitro. J Biomed Biotechnol. 2012; 2012: 721285.
11. Huang CC, Lo CP, Chiu CY, Shayur LF. Deoxyelephantopin, a novel multifunctional agent, suppresses mammary tumour growth and lung metastasis and doubles survival time in mice. Br J Pharmacol. 2010; 159(4): 856-871.
12. Su M, Wu X, Chung HY, Li Y, Ye W. Antiproliferative activities of five Chinese medicinal herbs and active compounds in Elephantopus scaber. Nat Prod Commun. 2009; 4(8): 1025-1230.
13. Than NN, Fotso S, Sevvana M, Sheldrick GM, Fiebig HH, Kelter G, Laatsch H. Sesquiterpene lactones from Elephantopus scaber. Z Naturforsch. 2005; 60b: 200-204.
14. Su M, Chung HY, Li Y. Deoxyelephantopin from Elephantopus scaber L. induces cell-cycle arrest and apoptosis in the human nasopharyngeal cancer CNE cells. Biochem Biophys Res Commun. 2011; 411(2): 342-347.
15. Katz D, Ito E, Lau KS, Mocanu JD, Bastianutto C, Schimmer AD, Liu FF. Increased efficiency for performing colony formation assays in 96-well plates: novel applications to combination therapies and high-throughput screening. Biotechniques. 2008; 44(2): ix-xiv.
16. Liu RH. Potential synergy of phytochemicals in cancer prevention: mechanism of action. J Nutr. 2004; 134(12 Suppl): 3479S-3485S.
17. Xu G, Liang Q, Gong Z, Yu W, He S, Xi L. Antitumor activities of the four sesquiterpene lactones from Elephantopus scaber L. Exp Oncol. 2006, 28(2): 106-109.
18. Lee WL, Shyur LF. Deoxyelephantopin impedes mammary adenocarcinoma cell motility by inhibiting calpain-mediated adhesion dynamics and inducing reactive oxygen species and aggresome formation. Free Radic Biol Med. 2012; 52(8): 1423-1436.
19. Zhang S, Won YK, Ong CN, Shen HM. Anti-cancer potential of sesquiterpene lactones: bioactivity and molecular mechanisms. Curr Med Chem Anticancer Agents. 2005; 5(3): 239-249.
20. Varalakshmi KN, Sangeetha CG, Samee US, Irum G, Lakshmi H, Prachi SP. In vitro safety assessment of effect of five medicinal plants on human peripheral lymphocytes. Trop J Pharm Res. 2011; 10(1): 33-40.
21. Aithal BK, Kumar MR, Rao BN, Udupa N, Rao BS. Juglone, a naphthoquinone from walnut, exerts cytotoxic and genotoxic effects against cultured melanoma tumor cells. Cell Biol Int. 2009; 33(10): 1039-1049.
22. Budihardjo I, Oliver H, Lutter M, Luo X, Wang X. Biochemical pathways of caspase activation during apoptosis. Annu Rev Cell Dev Biol. 1999; 15: 269-290.
23. Robertson JD, Enoksson M, Suomela M, Zhivotovsky B, Orrenius S. Caspase-2 acts upstream of mitochondria to promote cytochrome c release during etoposide-induced apoptosis. J Biol Chem. 2002; 277(33): 29803-29809.
24. Li H, Bergeron L, Cryns V, Pasternack MS, Zhu H, Shi L, Greenberg A, Yuan J. Activation of caspase-2 in apoptosis. J Biol Chem. 1997; 272(34): 21010-21017.
25. Lavrik IN, Golks A, Baumann S, Krammer PH. Caspase-2 is activated at the CD95 death-inducing signaling complex in the course of CD95-induced apoptosis. Blood. 2006; 108(2): 559-565.
26. Nicholson DW, Ali A, Thornberry NA, Vaillancourt JP, Ding CK, Gallant M, Gareau Y, Griffin PR, Labelle M, Lazebnik YA, Munday NA, Raju SM, Smulson ME, Yamin TT, Yu WL, Miller DK. Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis. Nature. 1995; 376(6535): 37-43.
27. Carnero A. Targeting the cell cycle for cancer therapy. Br J Cancer. 2002; 87(2): 129-133.
28. Hartwell LH, Weinert TA. Checkpoints: controls that ensure the order of cell cycle events. Science. 1989; 246(4930): 629-634.
29. Lewin B. Driving the cell cycle: M phase kinases, its partners, and substrates. Cell. 1990; 61(5): 743-752.
30. Makin G, Hickman JA. Apoptosis and cancer chemotherapy. Cell Tissue Res. 2000; 301(1): 143-152.