Cytotoxic, genotoxic and the hemolytic effect of titanium dioxide (TiO2) nanoparticles on human erythrocyte and lymphocyte cells in vitro

Journal of Applied Toxicology

Volumn 33, Issue 10, 2013, Pages 1097-1110

  Ghosh, Manosij ^a   Chakraborty, Anirban ^a   Mukherjee, Anita ^a  

^a UNIVERSITY OF CALCUTTA   (India)

Author keywords

Atomic force microscopy; Cytotoxicity; Echinocyte; Genotoxicity; Hemolysis; Reactive oxygen species

Indexed keywords

HEMOGLOBIN; NANOPARTICLE; TITANIUM DIOXIDE;

ADULT; APOPTOSIS; ARTICLE; ATOMIC FORCE MICROSCOPY; CELL DEATH; CELL VIABILITY; COMET ASSAY; CONTROLLED STUDY; CYTOTOXICITY; CYTOTOXICITY TEST; DNA DAMAGE; DNA FRAGMENTATION; ENZYME ACTIVITY; ERYTHROCYTE; ERYTHROCYTE COUNT; ERYTHROCYTE MEMBRANE; ERYTHROCYTE METABOLISM; ERYTHROCYTE STRUCTURE; FLOW CYTOMETRY; GENOTOXICITY; HEMOLYSIS; HUMAN; HUMAN CELL; IN VITRO STUDY; LEUKOCYTE COUNT; LYMPHOCYTE; LYSOSOME MEMBRANE; MALE; MEAN CORPUSCULAR VOLUME; MEMBRANE DAMAGE; MITOCHONDRIAL MEMBRANE POTENTIAL; MOLECULAR STABILITY; OSMOTIC FRAGILITY; PRIORITY JOURNAL; PROTEIN INTERACTION; SPHEROCYTOSIS; THROMBOCYTE COUNT; TRANSMISSION ELECTRON MICROSCOPY; ULTRAVIOLET SPECTROPHOTOMETRY;

EID: 84880964950     PISSN: 0260437X     EISSN: 10991263     Source Type: Journal    
DOI: 10.1002/jat.2863     Document Type: Article

References (49)

1. Ahmed SA, Gogal RM, Walsh JE. 1994. A new rapid and simple non-radioactive assay to monitor and determine the proliferation of lymphocytes: an alternative to [3H] thymidine incorporation assay. J. Immunol. Method 170: 211-224.
2. Aisaka Y, Kawaguchi R, Watanabe S, Ikeda M, Igisu H. 2008. Hemolysis caused by titanium dioxide particles. Inhal. Toxicol. 20: 891-893.
3. Arora S, Rajwade JM, Paknikar KM. 2012. Nanotoxicology and in vitro studies: the need of the hour. Toxicol. Appl. Pharmacol. 258: 151-165.
4. Bernard BK, Osheroff MR, Hofmann A, Mennear JH. 1990. Toxicology and carcinogenesis studies of dietary titanium dioxide-coated mica in male and female Fischer 344 rats. J. Toxicol. Environ. Health 29: 417-429.
5. 2 on bottlenose dolphin leukocytes. Anal. Bioanal. Chem. 396: 619-623.
6. Bessman JD. 1990. Reticulocytes In Clinical Methods: The History, Physical, and Laboratory Examinations, Walker HK, Hall WD, Hurst JW (eds). Butterworths: Boston, MA; 735-738.
7. Bhattacharya J, Choudhuri U, Siwach O, Sen P, Dasgupta AK. 2006. Interaction of hemoglobin and copper nanoparticles: implications in hemoglobinopathy. Nanomedicine 2: 191-199.
8. Bhattacharya K, Davoren M, Boertz J, Schins RPF, Hoffmann E, Dopp E. 2009. Titanium dioxide nanoparticles induce oxidative stress and DNA-adduct formation but not DNA-breakage in human lung cells. Part. Fibre Toxicol. 6: 17.
9. Bonfoco E, Krainc D, Ankarcrona M, Nicotera P, Lipton SA. 1995. Apoptosis and necrosis: two distinct events induced, respectively, by mild and intense insults with N-methyl-D-aspartate or nitric oxide/superoxide in cortical cell cultures. PNAS 92: 7162-7166.
10. Borenfreunda E, Puerner JA. 1985. Toxicity determined in vitro by morphological alterations and neutral red absorption. Toxicol. Lett. 24: 119-124.
11. Boxall A, Tiede K, Chaudhry Q. 2007. Engineered nanomaterials in soils and water: how do they behave and could they pose a risk to human health. Nanomedicine 2: 919-927.
12. Boyum A. 1976. Isolation of lymphocytes, granulocytes and macrophages. Scand. J. Immunol. 5: 9-15.
13. Chen JL, Fayerweather WE. 1988. Epidemiologic study of workers exposed to titanium dioxide. J. Occup. Med. 30: 937-942.
14. Dhawan A, Sharma V. 2010. Toxicity assessment of nanomaterials: methods and challenges. Anal. Bioanal. Chem. 398: 589-605.
15. Driscoll KE, Deyo LC, Carter JM, Howard BW, Hassenbein DG, Bertram TA. 1997. Effects of particle exposure and particle-elicited inflammatory cellson mutation in rat alveolar epithelial cells. Carcinogenesis 18: 423-430.
16. 2 surfaces: Influence of surface topography and oxide thickness on leukocyte adhesion and activation. Biomaterials 22: 1987-1996.
17. 2. Hum. Exp. Toxicol. 28: 339-352.
18. Fields RD, Lancaster MV. 1993. Dual-attribute continuous monitoring of cell proliferation/ cytotoxicity. Am. Biotechnol. Lab. 11: 48-50.
19. Gheshlaghi ZN, Riazi GH, Ahmadian S, Ghafari M, Mahinpour R. 2008. Toxicity and interaction of titanium dioxide nanoparticles with microtubule protein. Acta Biochim. Biophys. Sin. 40: 777-782.
20. 2) nanoparticles at two trophic levels: Plant and human lymphocytes. Chemosphere 81: 1253-1262.
21. Goegan P, Johnson G, Vincent R. 1995. Effects of serum protein and colloid on AB assay in cell cultures. Toxic In Vitro 9: 257-266.
22. Hart GA, Hesterberg TW. 1998. In vitro toxicity of respirable-size particles of diatomaceous earth and crystalline silica compared with asbestos and titanium dioxide. J. Occup. Environ. Med. 40: 29-42.
23. Henderson L, Jones E, Brooks T, Chetelat A, Ciliutti P, Freemantle M, Howard CA, Mackay J, Phillips B, Riley S, Roberts C, Wotton AK, van de Waart EJ. 1997. Industrial genotoxicology group collaborative trial to investigate cell cycle parameters in human lymphocyte cytogenetic studies. Mutagenesis 12: 163-167.
24. 2 long term exposure. Toxicol. Appl. Pharmacol. 241: 182-194.
25. Iavicoli I, Leso V, Fontana L, Bergamaschi A. 2011. Toxicological effects of titanium dioxide nanoparticles: a review of in vitro mammalian studies. Eur. Rev. Med. Pharmacol. Sci. 15: 481-508.
26. Jin CY, Zhu BS, Wang XF, Lu QH. 2008. Cytotoxicity of titanium dioxide nanoparticles in mouse fibroblast cells. Chem. Res. Toxicol. 21: 1871-1877.
27. Kang SJ, Kim BM, Lee YJ, Chung HW. 2008. Titanium dioxide nanoparticle trigger p53-mediated damage response in peripheral blood lymphocytes. Environ. Mol. Mutagen. 49: 399-405.
28. Katsu T, Kuroko M, Morikawa T. 1989. Mechanism of membrane damage induced by the amphipatic peptides gramicidin S and melittin. Biochim. Biophys. Acta 983: 135-141.
29. Linnainmaa K, Kivipensas P, Vainio H. 1997. Toxicity and cytogenetic studies of ultrafine titanium dioxide in cultured rat liver epithelial cells. Toxicol. In Vitro 11: 329-335.
30. Mosmann T. 1983. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods 65: 55-63.
31. Nociari MM, Shalev A, Benias P, Russo C. 1998. A novel one-step, highly sensitive fluorometric assay to evaluate cell-mediated cytotoxicity. J. Immunol. Methods 213: 157-167.
32. 2 surfaces. J. Lab. Clin. Med. 129: 35-46.
33. O'Brien J, Wilson I, Orton T, Pognan F. 2000. Investigation of the Alamar blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity. Eur. J. Biochem. 267: 5421-5426.
34. 2) nanoparticle- induced cytotoxicity and oxidative DNA damage in fish cells. Mutat. Res. 640: 113-122.
35. Shi Y, Zhang JH, Jiang M, Zhu LH, Tan HQ, Lu B. 2010. Synergistic genotoxicity caused by low concentration of titanium dioxide nanoparticles and p, p'-DDT in human hepatocytes. Environ. Mol. Mutagen. 51: 192-204.
36. Singh NP, McCoy MT, Tice RR, Schneider EL. 1988. A simple technique for quantification of low levels of DNA damage in individual cells. Exp. Cell Res. 175: 184-191.
37. Susheela AK, Jain SK. 1985. Fluoride toxicity: erythrocyte membrane abnormality and " echinocyte " formation. Fluoride Res. Stud. Environ. Sci. 27: 231-239.
38. Tennant JR. 1964. Evaluation of the trypan blue technique for determination of cell viability. Transplantation 2: 685-694.
39. 2 nanoparticles. Nanomedicine 4: 226-236.
40. Tice RR, Agurell E, Anderson D, Burlinson B, Hartmann A, Kobayashi H, Miyamae Y, Rojas E, Ryu JC, Sasaki YF. 2000. Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing. Environ. Mol. Mutagen. 35: 206-221.
41. Trouiller B, Reliene R, Westbrook A, Solaimani P, Schiestl RH. 2009. Titanium dioxide nanoparticles induce DNA damage and genetic instability in vivo in mice. Cancer Res. 69: 8784-8789.
42. Turkez H, Geyikoglu F. 2007. An in vitro blood culture for evaluating the genotoxicity of titanium dioxide: the responses of antioxidant enzymes. Toxicol. Ind. Health 23: 19-23.
43. 2 nanoparticles: studies on a human monoblastoid cell line. Toxicol. In Vitro 22: 1689-1696.
44. 2) nanoparticles on fish cells in vitro. Ecotoxicology 17: 410-420.
45. 2 particles in cultured human lymphoblastoid cells. Mutat. Res. 628: 99-106.
46. 2 nanoparticles in hairless mice and porcine skin after sub chronic dermal exposure. Toxicol. Lett. 191: 1-8.
47. Xu A, Chai Y, Nohmi T, Hei TK. 2009. Genotoxic response to titanium dioxide nanoparticles and fullerene in gpt transgenic MEF cells. Part. Fiber Toxicol. 6: 3.
48. 2) nanoparticles induce JB6 cell apoptosis through activation of the caspase-8/Bid and mitochondrial pathways. J. Toxicol. Environ. Health A 72: 1141-1149.
49. 2 on plasmid DNA and pepsin. In The 2nd International Conference on Bioinformatics and Biomedical Engineering. Shanghai, China, 1013-1016.