Codon optimization of Caenorhabditis elegans GluCl ion channel genes for mammalian cells dramatically improves expression levels

Journal of Neuroscience Methods

Volumn 124, Issue 1, 2003, Pages 75-81

  Slimko, Eric M ^a   Lester, Henry A ^a  

^a CALIFORNIA INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

Codon optimization; ECFP; Electrophysiology; Excitability; EYFP; Fusion proteins; GluCl

Indexed keywords

CHLORIDE CHANNEL; COMPLEMENTARY DNA; CYAN FLUORESCENT PROTEIN; GLUTAMATE CHLORIDE CHANNEL PROTEIN; GLUTAMIC ACID; IVERMECTIN; MEMBRANE PROTEIN; UNCLASSIFIED DRUG; YELLOW FLUORESCENT PROTEIN; GLUTAMATE GATED CHLORIDE CHANNELS; GLUTAMATE-GATED CHLORIDE CHANNELS; HYBRID PROTEIN;

AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; BRAIN NERVE CELL; CAENORHABDITIS ELEGANS; CODON USAGE; CONTROLLED STUDY; GENE EXPRESSION REGULATION; GENE MUTATION; HIPPOCAMPUS; MAMMAL CELL; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN ENGINEERING; RAT; ANIMAL; CELL LINE; CLASSIFICATION; CODON; COMPARATIVE STUDY; CYTOLOGY; DRUG EFFECT; DRUG POTENTIATION; ELECTROPHYSIOLOGY; GENETIC TRANSFECTION; GENETICS; METABOLISM; METHODOLOGY; NERVE CELL; PHYSIOLOGY; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; SITE DIRECTED MUTAGENESIS; SPECIES DIFFERENCE;

CAENORHABDITIS ELEGANS; XENOPUS;

ANIMALS; CAENORHABDITIS ELEGANS; CELL LINE; CHLORIDE CHANNELS; CODON; ELECTROPHYSIOLOGY; GENE EXPRESSION REGULATION; HIPPOCAMPUS; IVERMECTIN; MUTAGENESIS, SITE-DIRECTED; NEURONS; PROTEIN ENGINEERING; RATS; RECOMBINANT FUSION PROTEINS; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; SPECIES SPECIFICITY; TRANSFECTION;

EID: 0037473220     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-0270(02)00362-X     Document Type: Article

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