Self-organization and the self-assembling process in Tissue engineering

Annual Review of Biomedical Engineering

Volumn 15, Issue , 2013, Pages 115-136

  Athanasiou, Kyriacos A ^a   Eswaramoorthy, Rajalakshmanan ^a   Hadidi, Pasha ^a   Hu, Jerry C ^a  

^a University of California   (United States)

Author keywords

clinical translation; regenerative medicine; scaffoldless; self assembling process; self organization; tissue engineering

Indexed keywords

CLINICAL TRANSLATION; REGENERATIVE MEDICINE; SCAFFOLDLESS; SELF ORGANIZATIONS; SELF-ASSEMBLING PROCESS;

HISTOLOGY; TISSUE ENGINEERING;

TISSUE;

CADHERIN; CELL ADHESION MOLECULE; COLLAGEN TYPE 7; LAMININ; NERVE CELL ADHESION MOLECULE;

ARTICULAR CARTILAGE; BIOPRINTING; BONE; CELL ADHESION; CELL ENERGY; CELL INTERACTION; CELL REGENERATION; CELL RENEWAL; CELL SELECTION; CLINICAL TRIAL (TOPIC); CORNEA; CRYSTALLIZATION; EYE; FIBROCARTILAGE; HEART MUSCLE; HUMAN; KNEE MENISCUS; LIGAMENT; LIVER; MORPHOGENESIS; NERVE; NONHUMAN; PHENOTYPE; PROTEIN FOLDING; REGENERATIVE MEDICINE; REVIEW; SELF ASSEMBLING PROCESS; SELF ORGANIZATION PROCESS; TENDON; TISSUE ENGINEERING; VASCULARIZATION;

ANIMALS; BIOMIMETICS; CELL CULTURE TECHNIQUES; CLINICAL TRIALS AS TOPIC; DISEASE MODELS, ANIMAL; HUMANS; REGENERATIVE MEDICINE; THERMODYNAMICS; TISSUE ENGINEERING; TISSUE SCAFFOLDS;

EID: 84880524250     PISSN: 15239829     EISSN: 15454274     Source Type: Book Series    
DOI: 10.1146/annurev-bioeng-071812-152423     Document Type: Review

References (123)

1. Sanchez-Adams J, Athanasiou KA. 2012. Regional effects of enzymatic digestion on knee meniscus cell yield and phenotype for tissue engineering. Tissue Eng. Part C Methods 18:235-43
2. Sanchez-Adams J, Athanasiou KA. 2012. Dermis isolated adult stem cells for cartilage tissue engineering. Biomaterials 33:109-19
3. Natoli RM, Skaalure S, Bijlani S, Chen KX, Hu J, Athanasiou KA. 2010. Intracellular Na+ and Ca2+ modulation increases the tensile properties of developing engineered articular cartilage. Arthritis Rheum. 62:1097-107
4. Responte DJ, Arzi B, Natoli RM, Hu JC, Athanasiou KA. 2012. Mechanisms underlying the synergistic enhancement of self-Assembled neocartilage treated with chondroitinase-ABC andTGF-β1. Biomaterials 33:3187-94
5. Discher DE, Janmey P, Wang YL. 2005. Tissue cells feel and respond to the stiffness of their substrate. Science 310:1139-43
6. Leong MF, Rasheed MZ, Lim TC, Chian KS. 2009. In vitro cell infiltration and in vivo cell infiltration and vascularization in a fibrous, highly porous poly(D,L-lactide) scaffold fabricated by cryogenic electrospinning technique. J. Biomed. Mater. Res. A 91:231-40
7. Rosso F, Giordano A, BarbarisiM, Barbarisi A. 2004. From cell-ECM interactions to tissue engineering. J. Cell Physiol. 199:174-80
8. Zhao X, Kim J, Cezar CA, Huebsch N, Lee K, et al. 2011. Active scaffolds for on-Demand drug and cell delivery. Proc. Natl. Acad. Sci. USA 108:67-72
9. Huey DJ, Hu JC, Athanasiou KA. 2012. Unlike bone, cartilage regeneration remains elusive. Science 338:917-21
10. Liu X, Ma PX. 2004. Polymeric scaffolds for bone tissue engineering. Ann. Biomed. Eng. 32:477-86
11. Levy-Mishali M, Zoldan J, Levenberg S. 2009. Effect of scaffold stiffness on myoblast differentiation. Tissue Eng. Part A 15:935-44
12. Bryant SJ, Anseth KS, Lee DA, Bader DL. 2004. Crosslinking density influences the morphology of chondrocytes photoencapsulated in PEG hydrogels during the application of compressive strain. J. Orthop. Res. 22:1143-49
13. Zhang Z, McCaffery JM, Spencer RG, Francomano CA. 2004. Hyaline cartilage engineered by chondrocytes in pellet culture: Histological, immunohistochemical and ultrastructural analysis in comparison with cartilage explants. J. Anat. 205:229-37
14. Furukawa KS, Suenaga H, Toita K, Numata A, Tanaka J, et al. 2003. Rapid and large-scale formation of chondrocyte aggregates by rotational culture. Cell Transplant. 12:475-79
15. Vunjak-NovakovicG,Martin I,Obradovic B, Treppo S, Grodzinsky AJ, et al. 1999. Bioreactor cultivation conditions modulate the composition and mechanical properties of tissue-engineered cartilage. J. Orthop. Res. 17:130-38
16. Meredith JC, Sormana JL, Keselowsky BG, Garcia AJ, Tona A, et al. 2003. Combinatorial characterization of cell interactions with polymer surfaces. J. Biomed. Mater. Res. A 66:483-90
17. Yoon DM, Fisher JP. 2006. Chondrocyte signaling and artificial matrices for articular cartilage engineering. Adv. Exp. Med. Biol. 585:67-86
18. Barbero A, Grogan SP, Mainil-Varlet P, Martin I. 2006. Expansion on specific substrates regulates the phenotype and differentiation capacity of human articular chondrocytes. J. Cell Biochem. 98:1140-49
19. Elder SH, Sanders SW, McCulley WR, Marr ML, Shim JW, Hasty KA. 2006. Chondrocyte response to cyclic hydrostatic pressure in alginate versus pellet culture. J. Orthop. Res. 24:740-47
20. Anderson JM. 2001. Biological responses to materials. Annu. Rev. Mater. Res. 31:81-110
21. Jacobs HO,Tao AR, Schwartz A,Gracias DH,WhitesidesGM. 2002. Fabrication of a cylindrical display by patterned assembly. Science 296:323-25
22. Hartgerink JD, Beniash E, Stupp SI. 2001. Self-Assembly and mineralization of peptide-Amphiphile nanofibers. Science 294:1684-88
23. Whitesides GM, Grzybowski B. 2002. Self-Assembly at all scales. Science 295:2418-21
24. Mironov V, Visconti RP, Kasyanov V, Forgacs G, Drake CJ, Markwald RR. 2009. Organ printing: Tissue spheroids as building blocks. Biomaterials 30:2164-74
25. Eiraku M, Takata N, Ishibashi H, Kawada M, Sakakura E, et al. 2011. Self-organizing optic-cup morphogenesis in three-Dimensional culture. Nature 472:51-56
26. Youssef J, Nurse AK, Freund LB, Morgan Jr. 2011. Quantification of the forces driving self-Assembly of three-Dimensional microtissues. Proc. Natl. Acad. Sci. USA 108:6993-98
27. Peck M, Gebhart D, Dusserre N, McAllister TN, L'Heureux N. 2012. The evolution of vascular tissue engineering and current state of the art. Cells Tissues Organs 195:144-58
28. Jakab K, Norotte C, Marga F, Murphy K, Vunjak-Novakovic G, Forgacs G. 2010. Tissue engineering by self-Assembly and bio-printing of living cells. Biofabrication 2:022001
29. Baltich J, Hatch-Vallier L, Adams AM, Arruda EM, Larkin LM. 2010. Development of a scaffoldless three-Dimensional engineered nerve using a nerve-fibroblast co-culture. In Vitro Cell. Dev. Biol. Anim. 46:438-44
30. Riccalton-Banks L, Liew C, Bhandari R, Fry J, Shakesheff K. 2003. Long-Term culture of functional liver tissue: Three-Dimensional coculture of primary hepatocytes and stellate cells. Tissue Eng. 9:401-10
31. Halley JD, Winkler DA. 2008. Consistent concepts of self-organization and self-Assembly. Complexity 14:10-17
32. John K, Bar M. 2005. Alternative mechanisms of structuring biomembranes: Self-Assembly versus selforganization. Phys. Rev. Lett. 95:198101
33. Calve S, Dennis RG, Kosnik PE II, Baar K, Grosh K, Arruda EM. 2004. Engineering of functional tendon. Tissue Eng. 10:755-61
34. Smietana MJ, Syed-Picard FN, Ma J, Kostrominova T, Arruda EM, Larkin LM. 2009. The effect of implantation on scaffoldless three-Dimensional engineered bone constructs. In Vitro Cell. Dev. Biol. Anim. 45:512-22
35. L'HeureuxN, Paquet S,Labbe R, Germain L, Auger FA. 1998.Acompletely biological tissue-engineered human blood vessel. FASEB J. 12:47-56
36. Nishida K, Yamato M, Hayashida Y, Watanabe K, Yamamoto K, et al. 2004. Corneal reconstruction with tissue-engineered cell sheets composed of autologous oral mucosal epithelium. N. Engl. J. Med. 351:1187-96
37. Foty RA, Steinberg MS. 2005. The differential adhesion hypothesis: A direct evaluation. Dev. Biol. 278:255-63
38. Ṕerez-Pomares JM, Foty RA. 2006. Tissue fusion and cell sorting in embryonic development and disease: Biomedical implications. BioEssays 28:809-21
39. Syed-Picard FN, Larkin LM, Shaw CM, Arruda EM. 2009. Three-Dimensional engineered bone from bone marrow stromal cells and their autogenous extracellular matrix. Tissue Eng. Part A 15:187-95
40. Paxton JZ, Grover LM, Baar K. 2010. Engineering an in vitro model of a functional ligament from bone to bone. Tissue Eng. Part A 16:3515-25
41. Norotte C, Marga FS, Niklason LE, Forgacs G. 2009. Scaffold-free vascular tissue engineering using bioprinting. Biomaterials 30:5910-17
42. Proulx S, d'Arc Uwamaliya J, Carrier P, Deschambeault A, Audet C, et al. 2010. Reconstruction of a human cornea by the self-Assembly approach of tissue engineering using the three native cell types. Mol. Vis. 16:2192-201
43. Shimizu T, Yamato M, Kikuchi A, Okano T. 2003. Cell sheet engineering for myocardial tissue reconstruction. Biomaterials 24:2309-16
44. Hu JC, Athanasiou KA. 2006. A self-Assembling process in articular cartilage tissue engineering. Tissue Eng. 12:969-79
45. Hoben GM, Hu JC, James RA, Athanasiou KA. 2007. Self-Assembly of fibrochondrocytes and chondrocytes for tissue engineering of the knee meniscus. Tissue Eng. 13:939-46
46. Steinberg MS. 1970. Does differential adhesion govern self-Assembly processes in histogenesis? Equilibrium configurations and the emergence of a hierarchy among populations of embryonic cells. J. Exp. Zool. 173:395-433
47. Steinberg MS. 2007. Differential adhesion in morphogenesis: A modern view. Curr. Opin. Genet. Dev. 17:281-86
48. Krieg M, Arboleda-Estudillo Y, Puech PH, Kafer J, Graner F, et al. 2008. Tensile forces govern germlayer organization in zebrafish. Nat. Cell Biol. 10:429-36
49. Brodland GW. 2002. The Differential Interfacial TensionHypothesis (DITH): A comprehensive theory for the self-rearrangement of embryonic cells and tissues. J. Biomech. Eng. 124:188-97
50. Harris AK. 1976. Is cell sorting caused by differences in the work of intercellular adhesion? A critique of the Steinberg hypothesis. J. Theor. Biol. 61:267-85
51. Dean DM, Morgan Jr. 2008. Cytoskeletal-mediated tension modulates the directed self-Assembly of microtissues. Tissue Eng. Part A 14:1989-97
52. Berrier AL, Yamada KM. 2007. Cell-matrix adhesion. J. Cell. Physiol. 213:565-73
53. Maitre JL, Berthoumieux H, Krens SF, Salbreux G, Julicher F, et al. 2012. Adhesion functions in cell sorting by mechanically coupling the cortices of adhering cells. Science 338:253-56
54. Evans E, Yeung A. 1989. Apparent viscosity and cortical tension of blood granulocytes determined by micropipet aspiration. Biophys. J. 56:151-60
55. Amack JD, Manning ML. 2012. Knowing the boundaries: Extending the differential adhesion hypothesis in embryonic cell sorting. Science 338:212-15
56. Ofek G, Revell CM, Hu JC, Allison DD, Grande-Allen KJ, Athanasiou KA. 2008. Matrix development in self-Assembly of articular cartilage. PLoS One 3:e2795
57. Oberlender SA, Tuan RS. 1994. Spatiotemporal profile of N-cadherin expression in the developing limb mesenchyme. Cell Commun. Adhes. 2:521-37
58. Kinoshita N, Sasai N, Misaki K, Yonemura S. 2008. Apical accumulation of Rho in the neural plate is important for neural plate cell shape change and neural tube formation. Mol. Biol. Cell 19:2289-99
59. Tzanakakis ES, Hansen LK, Hu WS. 2001. The role of actin filaments and microtubules in hepatocyte spheroid self-Assembly. Cell Motil. Cytoskelet. 48:175-89
60. Dean DM, Morgan Jr. 2008. Cytoskeletal-mediated tension modulates the directed self-Assembly of microtissues. Tissue Eng. Part A 14:1989-97
61. Brodland GW, Chen HH. 2000. The mechanics of heterotypic cell aggregates: Insights from computer simulations. J. Biomech. Eng. 122:402-7
62. Strohman RC, Bayne E, Spector D, Obinata T, Micou-Eastwood J, Maniotis A. 1990. Myogenesis and histogenesis of skeletal muscle on flexible membranes in vitro. In Vitro Cell. Dev. Biol. 26:201-8
63. Hairfield-Stein M, England C, Paek HJ, Gilbraith KB, Dennis R, et al. 2007. Development of selfassembled, tissue-engineered ligament from bone marrow stromal cells. Tissue Eng. 13:703-10
64. Huang YC, Dennis RG, Larkin L, Baar K. 2005. Rapid formation of functional muscle in vitro using fibrin gels. J. Appl. Physiol. 98:706-13
65. Pirraco RP, Obokata H, Iwata T, Marques AP, Tsuneda S, et al. 2011. Development of osteogenic cell sheets for bone tissue engineering applications. Tissue Eng. Part A 17:1507-15
66. Calve S, Lytle IF, Grosh K, Brown DL, Arruda EM. 2010. Implantation increases tensile strength and collagen content of self-Assembled tendon constructs. J. Appl. Physiol. 108:875-81
67. Huang YC, Dennis RG, Baar K. 2006. Cultured slow versus fast skeletal muscle cells differ in physiology and responsiveness to stimulation. Am. J. Physiol. Cell Physiol. 291:C11-17
68. Donnelly K, Khodabukus A, Philp A, Deldicque L, Dennis RG, Baar K. 2010. A novel bioreactor for stimulating skeletal muscle in vitro. Tissue Eng. Part C Methods 16:711-18
69. Ma D, Ren L, Liu Y, Chen F, Zhang J, et al. 2010. Engineering scaffold-free bone tissue using bone marrow stromal cell sheets. J. Orthop. Res. 28:697-702
70. Hildebrandt C, Buth H, Thielecke H. 2011. A scaffold-free in vitro model for osteogenesis of human mesenchymal stem cells. Tissue Cell 43:91-100
71. Fyhrie DP, Vashishth D. 2000. Bone stiffness predicts strength similarly for human vertebral cancellous bone in compression and for cortical bone in tension. Bone 26:169-73
72. Gauvin R, Ahsan T, Larouche D, Levesque P, Dube J, et al. 2010. A novel single-step self-Assembly approach for the fabrication of tissue-engineered vascular constructs. Tissue Eng. Part A 16:1737-47
73. Mironov V, Kasyanov V. 2009. Emergence of clinical vascular tissue engineering. Lancet 373:1402-4
74. Aufderheide AC, Athanasiou KA. 2007. Assessment of a bovine co-culture, scaffold-free method for growing meniscus-shaped constructs. Tissue Eng. 13:2195-205
75. Gwyther TA, Hu JZ, Christakis AG, Skorinko JK, Shaw SM, et al. 2011. Engineered vascular tissue fabricated from aggregated smooth muscle cells. Cells Tissues Organs 194:13-24
76. Gwyther TA, Hu JZ, Billiar KL, Rolle MW. 2011. Directed cellular self-Assembly to fabricate cellderived tissue rings for biomechanical analysis and tissue engineering. J. Vis. Exp. (57):e3366
77. McAllister TN, MaruszewskiM, Garrido SA,Wystrychowski W, Dusserre N, et al. 2009. Effectiveness of haemodialysis access with an autologous tissue-engineered vascular graft: A multicentre cohort study. Lancet 373:1440-46
78. Haraguchi Y, Shimizu T, Sasagawa T, Sekine H, Sakaguchi K, et al. 2012. Fabrication of functional three-Dimensional tissues by stacking cell sheets in vitro. Nat. Protoc. 7:850-58
79. Yang X, Mironov V,WangQ. 2012. Modeling fusion of cellular aggregates in biofabrication using phase field theories. J. Theor. Biol. 303:110-18
80. Baar K, Birla R, Boluyt MO, Borschel GH, Arruda EM, Dennis RG. 2005. Self-organization of rat cardiac cells into contractile 3-D cardiac tissue. FASEB J. 19:275-77
81. Kelm JM, Ehler E, Nielsen LK, Schlatter S, Perriard JC, Fussenegger M. 2004. Design of artificial myocardial microtissues. Tissue Eng. 10:201-14
82. Adams AM, Arruda EM, Larkin LM. 2012. Use of adipose-Derived stem cells to fabricate scaffoldless tissue-engineered neural conduits in vitro. Neuroscience 201:349-56
83. GriffithM, JacksonWB, Lagali N, Merrett K, Li F, Fagerholm P. 2009. Artificial corneas: A regenerative medicine approach. Eye (Lond.) 23:1985-89
84. Rothermel A, Biedermann T, Weigel W, Kurz R, Ruffer M, et al. 2005. Artificial design of threedimensional retina-like tissue from dissociated cells of the mammalian retina by rotation-mediated cell aggregation. Tissue Eng. 11:1749-56
85. Zhang W, Xiao J, Li C, Wan P, Liu Y, et al. 2011. Rapidly constructed scaffold-free cornea epithelial sheets for ocular surface reconstruction. Tissue Eng. Part C Methods 17:569-77
86. KoideN, Sakaguchi K, KoideY, Asano K, Kawaguchi M, et al. 1990. Formation ofmulticellular spheroids composed of adult rat hepatocytes in disheswith positively charged surfaces and under other nonadherent environments. Exp. Cell Res. 186:227-35
87. Torisawa YS, Takagi A, Nashimoto Y, Yasukawa T, Shiku H, Matsue T. 2007. A multicellular spheroid array to realize spheroid formation, culture, and viability assay on a chip. Biomaterials 28:559-66
88. Landry J, Bernier D, Ouellet C, Goyette R, Marceau N. 1985. Spheroidal aggregate culture of rat liver cells: Histotypic reorganization, biomatrix deposition, and maintenance of functional activities. J. Cell Biol. 101:914-23
89. Fukuda J, Sakai Y,NakazawaK. 2006. Novel hepatocyte culture system developed using microfabrication and collagen/polyethylene glycol microcontact printing. Biomaterials 27:1061-70
90. Hansen LK, Hsiao CG, Friend JR, Wu FJ, Bridge GA, et al. 1998. Enhanced morphology and function in hepatocyte spheroids: A model of tissue self-Assembly. Tissue Eng. 4:65-74
91. Ohashi K, Yokoyama T, Yamato M, Kuge H, Kanehiro H, et al. 2007. Engineering functional two-And three-Dimensional liver systems in vivo using hepatic tissue sheets. Nat. Med. 13:880-85
92. Meng Q, Wu D, Zhang G, Qiu H. 2006. Direct self-Assembly of hepatocytes spheroids within hollow fibers in presence of collagen. Biotechnol. Lett. 28:279-84
93. Abu-Absi SF, Friend JR, Hansen LK, HuWS. 2002. Structural polarity and functional bile canaliculi in rat hepatocyte spheroids. Exp. Cell Res. 274:56-67
94. Novotny JE, Turka CM, Jeong C,Wheaton AJ, Li C, et al. 2006. Biomechanical andmagnetic resonance characteristics of a cartilage-like equivalent generated in a suspension culture. Tissue Eng. 12:2755-64
95. Brehm W, Aklin B, Yamashita T, Rieser F, Trub T, et al. 2006. Repair of superficial osteochondral defects with an autologous scaffold-free cartilage construct in a caprine model: Implantation method and short-Term results. Osteoarthr. Cartil. 14:1214-26
96. Huey DJ, Athanasiou KA. 2011. Maturational growth of self-Assembled, functional menisci as a result of TGF-β1 and enzymatic chondroitinase-ABC stimulation. Biomaterials 32:2052-58
97. Huey DJ, Athanasiou KA. 2011. Tension-compression loading with chemical stimulation results in additive increases to functional properties of anatomic meniscal constructs. PLoS One 6:e27857
98. Elder BD, Athanasiou KA. 2008. Synergistic and additive effects of hydrostatic pressure and growth factors on tissue formation. PLoS One 3:e2341
99. Elder BD, Athanasiou KA. 2009. Effects of temporal hydrostatic pressure on tissue-engineered bovine articular cartilage constructs. Tissue Eng. Part A 15:1151-58
100. MaJ, GobleK, SmietanaM,KostrominovaT,LarkinL,Arruda EM. 2009. Morphological and functional characteristics of three-Dimensional engineered bone-ligament-bone constructs following implantation. J. Biomech. Eng. 131:101017
101. Kostrominova TY, Calve S, Arruda EM, Larkin LM. 2009. Ultrastructure of myotendinous junctions in tendon-skeletal muscle constructs engineered in vitro. Histol. Histopathol. 24:541-50
102. LarkinLM,Calve S,KostrominovaTY, ArrudaEM.2006. Structure and functional evaluation of tendonskeletal muscle constructs engineered in vitro. Tissue Eng. 12:3149-58
103. L'HeureuxN, Stoclet JC, Auger FA, Lagaud GJ, Germain L, Andriantsitohaina R. 2001. A human tissueengineered vascular media: A new model for pharmacological studies of contractile responses. FASEB J. 15:515-24
104. L'Heureux N, Dusserre N, Marini A, Garrido S, de la Fuente L, McAllister T. 2007. Technology insight: The evolution of tissue-engineered vascular grafts-from research to clinical practice. Nat. Clin. Pract. Cardiovasc. Med. 4:389-95
105. L'Heureux N,McAllister TN, de la Fuente LM. 2007. Tissue-engineered blood vessel for adult arterial revascularization. N. Engl. J. Med. 357:1451-53
106. Wystrychowski W, Cierpka L, Zagalski K, Garrido S, Dusserre N, et al. 2011. Case study: First implantation of a frozen, devitalized tissue-engineered vascular graft for urgent hemodialysis access. J. Vasc. Access 12:67-70
107. Yang J, Yamato M, Nishida K, Ohki T, Kanzaki M, et al. 2006. Cell delivery in regenerative medicine: The cell sheet engineering approach. J. Control. Release 116:193-203
108. Nishida K, Yamato M, Hayashida Y, Watanabe K, Yamamoto K, et al. 2004. Corneal reconstruction with tissue-engineered cell sheets composed of autologous oral mucosal epithelium. N. Engl. J. Med. 351:1187-96
109. Nishida K. 2003. Tissue engineering of the cornea. Cornea 22:S28-34
110. Nishida K, Yamato M, Hayashida Y, Watanabe K, Maeda N, et al. 2004. Functional bioengineered corneal epithelial sheet grafts from corneal stem cells expanded ex vivo on a temperature-responsive cell culture surface. Transplantation 77:379-85
111. Zieske JD. 2001. Extracellular matrix and wound healing. Curr. Opin. Ophthalmol. 12:237-41
112. Carrier P, Deschambeault A, Talbot M, Giasson CJ, Auger FA, et al. 2008. Characterization of wound reepithelialization using a new human tissue-engineered corneal wound healing model. Investig. Ophthalmol. Vis. Sci. 49:1376-85
113. Dua HS, Forrester JV. 1987. Clinical patterns of corneal epithelial wound healing. Am. J. Ophthalmol. 104:481-89
114. Hardt SE, Sadoshima J. 2004. Negative regulators of cardiac hypertrophy. Cardiovasc. Res. 63:500-9
115. Chung CY, Bien H, Sobie EA, Dasari V, McKinnon D, et al. 2011. Hypertrophic phenotype in cardiac cell assemblies solely by structural cues and ensuing self-organization. FASEB J. 25:851-62
116. Koay EJ, Athanasiou KA. 2009. Development of serum-free, chemically defined conditions for human embryonic stem cell-Derived fibrochondrogenesis. Tissue Eng. Part A 15:2249-57
117. Zhang B, Yang S, Sun Z, Zhang Y, Xia T, et al. 2011. Humanmesenchymal stem cells induced by growth differentiation factor 5: An improved self-Assembly tissue engineering method for cartilage repair. Tissue Eng. Part C Methods 17:1189-99
118. Neagu A, Kosztin I, Jakab K, Barz B, Neagu M, et al. 2006. Computational modeling of tissue selfassembly. Mod. Phys. Lett. B 20:1217-31
119. Natoli RM, Revell CM, Athanasiou KA. 2009. Chondroitinase ABC treatment results in greater tensile properties of self-Assembled tissue-engineered articular cartilage. Tissue Eng. Part A 15:3119-28
120. Hoben GM, Willard VP, Athanasiou KA. 2009. Fibrochondrogenesis of hESCs: Growth factor combinations and cocultures. Stem Cells Dev. 18:283-92
121. Carrier P, Deschambeault A, Audet C, Talbot M, Gauvin R, et al. 2009. Impact of cell source on human cornea reconstructed by tissue engineering. Investig. Ophthalmol. Vis. Sci. 50:2645-52
122. Asher DM. 1999. The transmissible spongiform encephalopathy agents: Concerns and responses of United States regulatory agencies in maintaining the safety of biologics. Dev. Biol. Stand. 100:103-18
123. Wystrychowski W, Cierpka L, Zagalski K, Garrido S, Dusserre N, et al. 2011. Case study: First implantation of a frozen, devitalized tissue-engineered vascular graft for urgent hemodialysis access. J. Vasc. Access 12:67-70