Enhanced morphology and function in hepatocyte spheroids: A model of tissue self-assembly

Tissue Engineering

Volumn 4, Issue 1, 1998, Pages 65-74

  Hansen, Linda K ^a,b,c   Hsiao, Chang Chun ^b   Friend, Julie R ^b   Wu, Florence J ^b,d   Bridge, Gary A ^a   Remmel, Rory P ^b   Cerra, Frank B ^b,c   Hu, Wei Shou ^b  

^a Department of Laboratory Medicine ^*  (United States)

^b University of Minnesota   (United States)

^c UNIVERSITY OF MINNESOTA MEDICAL SCHOOL   (United States)

^d Advanced Tissue Sciences Liquidating Trust   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIN; BETA CATENIN; CADHERIN; CYTOCHROME P450; ETHOXYRESORUFIN DEETHYLASE; FIBRONECTIN; RESORUFIN;

ANIMAL CELL; ARTICLE; CELL ADHESION; CELL AGGREGATION; CELL JUNCTION; CELL STRUCTURE; CONTROLLED STUDY; CYTOSKELETON; LIVER CELL; MALE; MONOLAYER CULTURE; NONHUMAN; PRIORITY JOURNAL; RAT; SPHEROID CELL;

EID: 0031915980     PISSN: 10763279     EISSN: None     Source Type: Journal    
DOI: 10.1089/ten.1998.4.65     Document Type: Article

References (24)

1. Koide, N., Shinji, N., Tanabe, T., Asano, K., Kawaguchi, M., Sakaguchi, K., Koide, Y., Mori, M., and Tsuji, T. Continued high albumin production by multicellular spheroids of adult rat hepatocytes formed in the presence of liver-derived proteoglycans. Biochem. Biophys. Res. Commun. 161, 385, 1989.
2. Tong, J.Z., Bernard, O., and Alvarez, F. Long-term culture of rat liver cell spheroids in hormonally defined media. Exp. Cell Res. 189, 87, 1990.
3. Asano, K., Koide, N., and Tsuji, T. Ultrastructure of multicellular spheroids formed in the primary culture of adult rat hepatocytes. J. Clin. Electron Microsc. 22, 243, 1989.
4. Peshwa, M.V., Wu, F.J., Sharp, H.L., Cerra, F.B., and Hu, W.-S. Mechanistics of formation and ultrastructural evaluation of hepatocyte spheroids. In Vitro Cell Dev. Biol. 32, 197, 1996.
5. Wu, F.J., Peshwa, M.V., Cerra, F.B., and Hu, W.-S. Entrapment of hepatocyte spheroids in a hollow fiber bioreactor as a potential bioartificial liver. Tissue Eng. 1, 29, 1995.
6. Seglen, P.O. Preparation of isolated rat liver cells. Methods Cell Biol. 13, 29, 1976.
7. Mooney, D.J., Hansen, L.K., Vacanti, J.P., Langer, R., Farmer, S.R., and Ingber, D.E. Switching from differentiation to growth in hepatocytes: Control by extracellular matrix. J. Cell. Phys. 151, 497, 1992.
8. Wu, F., Friend, J.R., Hsiao, C.C., Zilliox, M.J., Ko, W.J., Cerra, F.B., and Hu, W.S. Efficient assembly of rat hepatocyte spheroids for tissue engineering applications. Biotechnol. Bioeng. 50, 404, 1996.
9. Peshwa, M.V., Wu, F.J., Follstad, B.D., Cerra, F.B., and Hu, W.-S. Kinetics of hepatocyte spheroid formation. Biotechnol. Prog. 10, 460, 1994.
10. Wu, F., Remmel, R.P., Cerra, F.B., and Hu, W.-S. Enhanced cytochrome P4501A1 activity of self-assembled rat hepatocyte spheroids. Cell Transpl. (accepted for publication).
11. Burke, M.D., Thompson, S., Weaver, R.J., Wolf, C.R., and Mayer, R.T. Cytochrome P450 specificities of alkoxyresorufin O-dealkylation in human and rat liver. Biochem. Pharmacol. 48, 923, 1994.
12. Cretton, E.M., and Sommadossi, J.-P. Modulation of 3′-azido-3′-deoxythymidine catabolism by probenecid and acetaminophen in freshly isolated rat hepatocytes. Biochem. Pharmacol. 42, 1475, 1991.
13. Moghe, P.V., Berthiaume, F., Ezzell, R.M., Toner, M., Tompkins, R.G., and Yarmush, M.L. Culture matrix configuration and composition in the maintenance of hepatocyte polarity and function. Biomaterials 17, 373, 1996.
14. Ezzell, R.M., Toner, M., Hendricks, K., Dunn, J.C.Y., Tompkins, R.G., and Yarmush, M.L. Effect of collagen gel configuration on the cytoskeleton in cultured rat hepatocytes. Exp. Cell Res. 208, 442, 1993.
15. LeCluyse, E.L., Audus, K.L., and Hochman, J.H. Formation of extensive canalicular networks by rat hepatocytes cultured in collagen-sandwich configuration. Am. J. Physiol. 266, C1764, 1994.
16. Marceau, N., Grenier, A., Noel, M., Mailhot, D., and Loranger, A. Modulation of cytokeratin and actin gene expression and fibrillar organization in cultured rat hepatocytes. Biochem. Cell Biol. 70, 1238, 1992.
17. Ishi, M., Washioka, H., Tonosaki, A., and Toyota, T. Regional orientation of actin filaments in the pericanalicular cytoplasm of rat hepatocytes. Gastroenterology 101, 1663, 1991.
18. Kawahara, H., and French, S.W. Role of cytoskeleton in canalicular contraction in cultured differentiated hepatocytes. Am. J. Pathol. 136, 521, 1990.
19. Graf, J., Gautam, A., and Boyer, J.L. Isolated rat hepatocyte couplets: A primary secretory unit for electrophysiologic studies of bile secretory function. Proc. Natl. Acad. Sci. U.S.A. 81, 6516, 1984.
20. Burke, M.D., Thompson, S., Elcombe, C.R., Halpert, J., Haaparanta, T., and Mayer, R.T. Ethoxy-, pentoxy-, and benzyloxyphenoxazones and homologues: A series of substrates to distinguish between different induced cytochromes P-450. Biochem. Pharmacol. 34, 3337, 1985.
21. Sidhu, J.S., Kavanagh, T.J., Reilly, M.T., and Omiecinski, C.J. Direct determination of functional activity of cytochrome P450IA1 and NADPH DT-diaphorase in hepatoma cell lines using noninvasive scanning laser cytometry. J. Toxicol. Environ. Health 40, 177, 1993.
22. Gumbiner, B.M. Cell adhesion: The molecular basis of tissue architecture and morphogenesis. Cell 84, 345, 1996.
23. Siegfried, E., Wilder, E.L., and Perrimon, N. Components of wingless signalling in Drosophila. Nature (London) 367, 76, 1994.
24. Fleming, T.P., and Johnson, M.H. From egg to epithelium. Annu. Rev. Cell Biol. 4, 459, 1988.