A novel strategy for enhancing extracellular secretion of recombinant proteins in Escherichia coli

Applied Microbiology and Biotechnology

Volumn 97, Issue 15, 2013, Pages 6705-6713

  Su, Lingqia ^a   Xu, Chenhua ^a   Woodard, Ronald W ^b   Chen, Jian ^a   Wu, Jing ^a  

^a JIANGNAN UNIVERSITY   (China)

^b UNIVERSITY OF MICHIGAN   (United States)

Author keywords

Co expression; Cytosolic protein; Extracellular secretion; Secretory protein; T. fusca cutinase

Indexed keywords

CO-EXPRESSION; CUTINASE; CYTOSOLIC PROTEINS; EXTRACELLULAR SECRETION; INDIVIDUAL CONTROL; LARGE-SCALE PRODUCTION; MEMBRANE PERMEABILITY; TREHALOSE SYNTHASE;

CYTOLOGY; ESCHERICHIA COLI; PHYSIOLOGY; RECOMBINANT PROTEINS;

GENE ENCODING;

AMYLASE; BETA GALACTOSIDASE; CUTINASE; RECOMBINANT PROTEIN; TREHALOSE; XYLAN ENDO 1,3 BETA XYLOSIDASE; XYLOSE ISOMERASE;

COMMERCIAL ACTIVITY; CYTOPLASM; ENZYME ACTIVITY; MEMBRANE; MICROBIAL COMMUNITY; PERMEABILITY; PROTEIN; SECRETION; SIGNALING;

ARTICLE; BACTERIUM CULTURE; CONTROLLED STUDY; CULTURE MEDIUM; ESCHERICHIA COLI; EXTRACELLULAR MATRIX; NONHUMAN; PROTEIN EXPRESSION; PROTEIN SECRETION;

ALPHA-AMYLASES; BASE SEQUENCE; BIOMASS; CLONING, MOLECULAR; CULTURE MEDIA; CYTOSOL; DNA PRIMERS; ESCHERICHIA COLI; GLUCOSYLTRANSFERASES; ISOMERASES; RECOMBINANT PROTEINS;

EID: 84880406149     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-013-4994-7     Document Type: Article

References (30)

1. Andersson H, von Heijne G (1991) A 30-residue-long "export initiation domain" adjacent to the signal sequence is critical for protein translocation across the inner membrane of Escherichia coli. Proc Natl Acad Sci U S A 88:9751-9754
2. Angkawidjaja C, Kuwahara K, Omori K, Koga Y, Takano K, Kanaya S (2006) Extracellular secretion of Escherichia coli alkaline phosphatase with a C-terminal tag by type I secretion system: purification and biochemical characterization. Protein Eng Des Sel 19:337-343
3. Cai Z, Xu W, Xue R, Lin Z (2008) Facile, reagentless and in situ release of Escherichia coli intracellular enzymes by heat-inducible autolytic vector for high-throughput screening. Protein Eng Des Sel 21:681-687
4. Chen S, Tong X, Woodard RW, Du GC, Wu J, Chen J (2008) Identification and characterization of bacterial cutinase. J Biol Chem 283:25854-25862
5. Chien LJ, Lee CK (2006) Synergistic effect of co-expressing D-amino acid oxidase with T7 lysozyme on self-disruption of Escherichia coli cell disruption. Biochem Eng J 28:17-22
6. Choi JH, Lee SY (2004) Secretory and extracellular production of recombinant proteins using Escherichia coli. Appl Microbiol Biot 64:625-635
7. Dische Z, Borenfreund E (1951) A new spectrophotometric method for the detection and determination of keto sugars and trioses. J Biol Chem 192:583-587
8. Gupta R, Gigras P, Mohapatra H, Goswami VK, Chauhan B (2003) Microbial alpha-amylases: a biotechnological perspective. Process Biochem 38:1599-1616
9. Hikita C, Mizushima S (1992) Effects of total hydrophobicity and length of the hydrophobic domain of a signal peptide on in vitro translocation efficiency. J Biol Chem 267:4882-4888
10. Jechlinger W, Szostak MP, Witte A, Lubitz W (1999) Altered temperature induction sensitivity of the lambda pR/cI857 system for controlled gene E expression in Escherichia coli. FEMS Microbiol Lett 173:347-352
11. Jong WSP, Sauri A, Luirink J (2010) Extracellular production of recombinant proteins using bacterial autotransporters. Curr Opin Biotech 21:646-652
12. Kujau MJ, Hoischen C, Riesenberg D, Gumpert J (1998) Expression and secretion of functional miniantibodies McPC603scFvDhlx in cell-wall-less L-form strains of Proteus mirabilis and Escherichia coli: a comparison of the synthesis capacities of L-form strains with an E. coli producer strain. Appl Microbiol Biotechnol 49:51-58
13. Li ZF, Li B, Liu ZG, Wang M, Gu ZB, Du GC, Wu J, Chen J (2009) Calcium leads to further increase in glycine-enhanced extracellular secretion of recombinant alpha-cyclodextrin glycosyltransferase in Escherichia coli. J Agric Food Chem 57:6231-6237
14. Marshall RO, Kooi ER (1957) Enzymatic conversion of D-glucose to D-fructose. Science 125:648-649
15. Martinez A, Ramirez OT, Valle F (1997) Improvement of culture conditions to overproduce beta-galactosidase from Escherichia coli in Bacillus subtilis. Appl Microbiol Biotechnol 47:40-45
16. Matos CF, Branston SD, Albiniak A, Dhanoya A, Freedman RB, Keshavarz-Moore E, Robinson C (2012) High-yield export of a native heterologous protein to the periplasm by the tat translocation pathway in Escherichia coli. Biotechnol Bioeng 109:2533-2542
17. Mergulhao FJ, Summers DK, Monteiro GA (2005) Recombinant protein secretion in Escherichia coli. Biotechnol Adv 23:177-202
18. Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31:426-428
19. Morita M, Asami K, Tanji Y, Unno H (2001) Programmed Escherichia coli cell lysis by expression of cloned T4 phage lysis genes. Biotechnol Prog 17:573-576
20. Narayanan N, Khan M, Chou CP (2010) Enhancing functional expression of heterologous lipase B in Escherichia coli by extracellular secretion. J Ind Microbiol Biotechnol 37:349-361
21. Ni Y, Chen R (2009) Extracellular recombinant protein production from Escherichia coli. Biotechnol Lett 31:1661-1670
22. Shin HD, Chen RR (2008) Extracellular recombinant protein production from an Escherichia coli lpp deletion mutant. Biotechnol Bioeng 101:1288-1296
23. Shokri A, Sanden AM, Larsson G (2003) Cell and process design for targeting of recombinant protein into the culture medium of Escherichia coli. Appl Microbiol Biotechnol 60:654-664
24. Simmons LC, Yansura DG (1996) Translational level is a critical factor for the secretion of heterologous proteins in Escherichia coli. Nat Biotechnol 14:629-634
25. Su L, Chen S, Yi L, Woodard RW, Chen J, Wu J (2012) Extracellular overexpression of recombinant Thermobifida fusca cutinase by alpha-hemolysin secretion system in E. coli BL21(DE3). Microb Cell Fact 11:8
26. Tukel SS, Alagoz D (2008) Catalytic efficiency of immobilized glucose isomerase in isomerization of glucose to fructose. Food Chem 111:658-662
27. van der Maarel MJ, van der Veen B, Uitdehaag JC, Leemhuis H, Dijkhuizen L (2002) Properties and applications of starch-converting enzymes of the alpha-amylase family. J Biotechnol 94:137-155
28. Wei YT, Zhu QX, Luo ZF, Lui FS, Chen FZ, Wang QY, Huang K, Meng JZ, Wang R, Huang RB (2004) Cloning, expression and identification of a new trehalose synthase gene from Thermobifida fusca genome. Acta Bioch Bioph Sin 36:477-484
29. Witte A, Lubitz W (1989) Biochemical characterization of phi X174-protein-E-mediated lysis of Escherichia coli. Eur J Biochem 180:393-398
30. Yoon SH, Kim SK, Kim JF (2010) Secretory production of recombinant proteins in Escherichia coli. Recent Pat Biotechnol 4:23-29