Extracellular overexpression of recombinant Thermobifida fusca cutinase by alpha-hemolysin secretion system in E. coli BL21(DE3)

Microbial Cell Factories

Volumn 11, Issue , 2012, Pages

  Su, Lingqia ^a   Chen, Sheng ^a   Yi, Li ^b   Woodard, Ronald W ^b   Chen, Jian ^a   Wu, Jing ^a  

^a JIANGNAN UNIVERSITY   (China)

^b UNIVERSITY OF MICHIGAN   (United States)

Author keywords

Alpha hemolysin secretion pathway; Cutinase; Extracellular production; Protein secretion

Indexed keywords

CUTINASE; SIGNAL PEPTIDE; THERMOBIFIDA FUSCA CUTINASE; UNCLASSIFIED DRUG; CARBOXYLESTERASE; CARRIER PROTEIN; ESCHERICHIA COLI PROTEIN; HEMOLYSIN; HLYA PROTEIN, E COLI; HLYD PROTEIN, E COLI; RECOMBINANT PROTEIN;

ALPHA HEMOLYSIN SECRETION SYSTEM; ARTICLE; BACTERIAL SECRETION SYSTEM; BACTERIAL TRANSLOCATION; CHEMICAL ANALYSIS; ENZYME ACTIVITY; ENZYME PURIFICATION; ESCHERICHIA COLI; NONHUMAN; PH; PROTEIN EXPRESSION; TEMPERATURE; ACTINOMYCETALES; BIOSYNTHESIS; ENZYME SPECIFICITY; ENZYMOLOGY; GENE VECTOR; GENETICS; METABOLISM; SECRETION; SECRETORY PATHWAY;

ESCHERICHIA COLI; THERMOBIFIDA FUSCA;

ACTINOMYCETALES; CARBOXYLIC ESTER HYDROLASES; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENETIC VECTORS; HEMOLYSIN PROTEINS; HYDROGEN-ION CONCENTRATION; MEMBRANE TRANSPORT PROTEINS; PROTEIN SORTING SIGNALS; RECOMBINANT PROTEINS; SECRETORY PATHWAY; SUBSTRATE SPECIFICITY; TEMPERATURE;

EID: 84855608783     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/1475-2859-11-8     Document Type: Article

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