Altered temperature induction sensitivity of the lambda p(R)/cI857 system for controlled gene E expression in Escherichia coli

FEMS Microbiology Letters

Volumn 173, Issue 2, 1999, Pages 347-352

  Jechlinger, Wolfgang ^a,b   Szostak, Michael P ^a,b   Witte, Angela ^a   Lubitz, Werner ^a  

^a UNIVERSITY OF VIENNA   (Austria)

^b EVAX Technologies   (Germany)

Author keywords

cI857 p(R); Candidate vaccine; Gene expression; PhiX174 gene E; Safety cassette

Indexed keywords

VACCINE;

ARTICLE; BACTERIOPHAGE LAMBDA; CYTOLYSIS; ESCHERICHIA COLI; GENE EXPRESSION; GENE REPRESSION; MUTAGENESIS; MUTATION; NONHUMAN; PLASMID; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; TEMPERATURE SENSITIVITY; THERMOSTABILITY;

BACTERIOLYSIS; BACTERIOPHAGE LAMBDA; BACTERIOPHAGE PHI X 174; BASE SEQUENCE; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, VIRAL; LAC OPERON; MOLECULAR SEQUENCE DATA; MUTATION; PLASMIDS; PROMOTER REGIONS (GENETICS); TEMPERATURE; VIRAL PROTEINS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0032970919     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(99)00094-4     Document Type: Article

References (27)

1. Szostak, M., Wanner, G. and Lubitz, W. (1990) Recombinant bacterial ghosts as vaccines. Res. Microbiol. 141, 1005-1007.
2. Szostak, M., Auer, T. and Lubitz, W. (1993) Immune response against recombinant bacterial ghosts carrying HIV-1 reverse transcriptase. In: Vaccines 93: Modern Approaches to New Vaccines Including Prevention of AIDS (Ginsberg, H.S., Brown, F., Chanock, R.M. and Lerner, R.A., Eds.), pp. 419-425. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
3. Szostak, M., Hensel, A., Eko, F.O., Klein, R., Auer, T., Mader, H., Haslberger, A., Bunka, S., Wanner, G. and Lubitz, W. (1996) Bacterial ghosts: non living candidate vaccines. J. Biotechnol. 44, 161-170.
4. Szostak, M. and Lubitz, W. (1991) Recombinant ghosts as multivaccine vehicles. In: Vaccines 91: Modern Approaches to New Vaccines Including Prevention of AIDS (Ginsberg, H.S., Brown, F., Chanock, R.M. and Lerner, R.A., Eds.), pp. 409-414. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
5. Hensel, A., van Leengoed, L.A.G., Szostak, M., Windt, H., Weissenböck, H., Stockhofe-Zurwieden, N., Katinger, A., Stadler, M., Ganter, M., Bunka, S., Papst, R. and Lubitz, W. (1996) Induction of protective immunity by aerosol or oral application of candidate vaccines in a dose-controlled pig aerosol infection model. J. Biotechnol. 44, 171-181.
6. Henrich, B., Lubitz, W. and Plapp, R. (1982) Lysis of Escherichia coli by induction of cloned φX174 genes. Mol. Gen. Genet. 185, 493-497.
7. Bläsi, U., Henrich, B. and Lubitz, W. (1985) Lysis of Escherichia coli by cloned gene E depends on its expression. J. Gen. Microbiol. 131, 1107-1114.
8. Witte, A. and Lubitz, W. (1989) Biochemical characterization of PhiX174 protein E-mediated lysis of Escherichia coli. Eur. J. Biochem. 180, 393-398.
9. Witte, A., Bläsi, U., Halfmann, G., Szostak, M., Wanner, G. and Lubitz, W. (1990) PhiX174 protein E-mediated lysis of Escherichia coli. Biochimie 72, 191-200.
10. Witte, A., Wanner, G., Bläsi, U., Halfmann, G., Szostak, M. and Lubitz, W. (1990) Endogenous transmembrane tunnel formation mediated by φX174 lysis protein E. J. Bacteriol. 172, 4109-4114.
11. Schön, P., Schrot, G., Wanner, G., Lubitz, W. and Witte, A. (1995) Two-stage model for integration of the lysis protein E of bacteriophage PhiX174 into cell envelope of Escherichia coli. FEMS Microbiol. Rev. 17, 207-212.
12. Witte, A., Wanner, G. and Lubitz, W. (1992) Dynamics of PhiX174 protein E-mediated lysis of Escherichia coli. Arch. Microbiol. 157, 381-388.
13. L promoter of coliphage lambda. Gene 15, 81-93.
14. Gough, J. and Murray, N. (1983) Sequence diversity among related genes for recognition of specific targets in DNA molecules. J. Mol. Biol. 166, 1-19.
15. Wu, T.H., Clarke, C.H. and Marinus, M.G. (1990) Specificity of Escherichia coli mutD and mutL mutator strains. Gene 87, 1-5.
16. Messing, J., Crea, R. and Seeburg, P.H. (1981) A system for shotgun DNA sequencing. Nucleic Acids Res. 9, 309-321.
17. Kunkel, T.A., Roberts, J.D. and Zakour, R.A. (1987) Rapid and efficient site-specific mutagenesis without phenotypic selection. Methods Enzymol. 154, 367-382.
18. Silhavy, T.J., Berman, M.L. and Enquist, L.W. (1984) Experiments with Gene Fusions. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
19. Simons, R.W., Houman, F. and Kleckner, N. (1987) Improved single and multicopy lac-based cloning vectors for protein and operon fusions. Gene 53, 85-96.
20. Yanish-Perron, C., Vieira, J. and Messing, J. (1985) Improved M13 vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33, 103-119.
21. Miller, J.H. (1972) Experiments in Molecular Genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
22. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd Edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
23. Hanahan, D. (1983) Studies on transformation of Escherichia coli with plasmids. J. Mol. Biol. 166, 557-580.
24. Hershberger, P.A. and deHaseth, P.L. (1991) RNA polymerase bound to the PR promoter of bacteriophage lambda inhibits open complex formation at the divergently transcribed PRM promoter: implications for an indirect mechanism of transcriptional activation by lambda repressor. J. Mol. Biol. 222, 479-494.
25. R-bound RNA polymerase with PRM function in vitro. Modulation by the bacteriophage lambda cI protein. J. Biol. Chem. 268, 8943-8948.
26. Fong, R.S.-C., Woody, S. and Gussin, G.N. (1993) Modulation of PRM activity by the lambda PR promoter in both the absence and presence of repressor. J. Mol. Biol. 232, 792-804.
27. R. J. Mol. Biol. 229, 37-51.