In-cell Western™ detection of organic cation transporters in bronchial epithelial cell layers cultured at an air-liquid interface on Transwell® inserts

Journal of Pharmacological and Toxicological Methods

Volumn 68, Issue 2, 2013, Pages 184-189

  Mukherjee, M ^a   Latif, M L ^a   Pritchard, D I ^a   Bosquillon, C ^a  

^a UNIVERSITY OF NOTTINGHAM   (United Kingdom)

Author keywords

Calu 3 cell line; Drug absorption; Drug transporters; Epithelium; In vitro models; Methods; Protein quantification; Transwell

Indexed keywords

FENOFIBRATE; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; NITRIC OXIDE; NITROPRUSSIDE SODIUM; ORGANIC CATION TRANSPORTER 1; ORGANIC CATION TRANSPORTER 2; ORGANIC CATION TRANSPORTER 3; PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR ALPHA; ROSIGLITAZONE;

ARTICLE; CELL CULTURE; CONTROLLED STUDY; EPITHELIUM CELL; HUMAN; HUMAN CELL; IMAGE ANALYSIS; IMAGING SYSTEM; IN CELL WESTERN ASSAY; IN VITRO STUDY; MOLECULAR IMAGING; PROTEIN ANALYSIS; PROTEIN DETERMINATION; PROTEIN EXPRESSION; PROTEIN FUNCTION; SIGNAL TRANSDUCTION; WESTERN BLOTTING;

AIR LIQUID INTERFACE; ALI; BOVINE SERUM ALBUMIN; BSA; CALU-3 CELL LINE; DRUG ABSORPTION; DRUG TRANSPORTERS; EPITHELIUM; FACS; FBS; FLUORESCENCE-ACTIVATED CELL SORTING; FOETAL BOVINE SERUM; GAPDH; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE; ICW; IN VITRO MODELS; IN-CELL WESTERN™; INFRA RED; IR; METHODS; NEAR INFRA-RED; NHBE; NIR; NORMAL HUMAN BRONCHIAL EPITHELIAL; OCT; ORGANIC CATION TRANSPORTER; PARAFORMALDEHYDE; PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR; PFA; PPAR; PROTEIN QUANTIFICATION; QPCR; QUANTITATIVE POLYMERASE CHAIN REACTION; ROOM TEMPERATURE; RT; SEM; SNP; SODIUM NITROPRUSSIDE; STANDARD ERROR OF THE MEAN; TEER; TRANS-EPITHELIAL ELECTRICAL RESISTANCE; TRANSWELL; W.R.T.; WB; WESTERN BLOT; WITH RESPECT TO;

BLOTTING, WESTERN; BRONCHI; CELL LINE; EPITHELIAL CELLS; FENOFIBRATE; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASES; HUMANS; NITROPRUSSIDE; ORGANIC CATION TRANSPORT PROTEINS; REPRODUCIBILITY OF RESULTS; THIAZOLIDINEDIONES;

EID: 84880375237     PISSN: 10568719     EISSN: 1873488X     Source Type: Journal    
DOI: 10.1016/j.vascn.2013.05.007     Document Type: Article

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