A hybrid high-speed atomic force-optical microscope for visualizing single membrane proteins on eukaryotic cells

Nature Communications

Volumn 4, Issue , 2013, Pages

  Colom, Adai ^a   Casuso, Ignacio ^a   Rico, Felix ^a   Scheuring, Simon ^a  

^a Université Aix Marseille   (France)

Author keywords

[No Author keywords available]

Indexed keywords

AQUAPORIN; MEMBRANE PROTEIN;

ATOMIC FORCE MICROSCOPY; CYTOLOGY; EUKARYOTE; MEMBRANE; MOLECULAR ANALYSIS; PROTEIN;

ARTICLE; ATOMIC FORCE MICROSCOPY; BRIGHT FIELD MICROSCOPY; CELL MEMBRANE; CYTOLOGY; DIMENSIONAL MEASUREMENT ACCURACY; DIODE LASER; EUKARYOTIC CELL; FLUORESCENCE MICROSCOPE; FLUORESCENCE MICROSCOPY; HIGH SPEED ATOMIC FORCE OPTICAL MICROSCOPE; LASER; LENS EPITHELIUM; MICROSCOPY; PROTEIN INTERACTION;

ANIMALS; AQUAPORINS; BIOLOGICAL TRANSPORT; CELL MEMBRANE; CELLS, IMMOBILIZED; ESCHERICHIA COLI; EYE PROTEINS; LENS, CRYSTALLINE; MICROSCOPY, ATOMIC FORCE; MICROSCOPY, FLUORESCENCE; MOLECULAR DYNAMICS SIMULATION; PRIMARY CELL CULTURE; SHEEP; SHEEP, DOMESTIC; TIME-LAPSE IMAGING;

EID: 84880364773     PISSN: None     EISSN: 20411723     Source Type: Journal    
DOI: 10.1038/ncomms3155     Document Type: Article

References (29)

1. Ando, T. et al. A high-speed atomic force microscope for studying biological macromolecules. Proc. Natl Acad. Sci. USA 98, 12468-12472 (2001). (Pubitemid 33019969)
2. Casuso, I., Rico, F. & Scheuring, S. High-speed atomic force microscopy: structure and dynamics of single proteins. Curr. Opin. Chem. Biol. 15, 704-709 (2011).
3. Kodera, N., Yamamoto, D., Ishikawa, R. & Ando, T. Video imaging of walking myosin V by high-speed atomic force microscopy. Nature 468, 72-76 (2010).
4. Igarashi, K. et al. Traffic jams reduce hydrolytic efficiency of cellulase on cellulose surface. Science 333, 1279-1282 (2011).
5. Uchihashi, T., Iino, R., Ando, T. & Noji, H. High-Speed atomic force microscopy reveals rotary catalysis of rotorless F1-ATPase. Science 333, 755-758 (2011).
6. Shibata, M., Yamashita, H., Uchihashi, T., Kandori, H. & Ando, T. High-speed atomic force microscopy shows dynamic molecular processes in photoactivated bacteriorhodopsin. Nat. Nano. 5, 208-212 (2010).
7. Casuso, I. et al. Characterization of the motion of membrane proteins using high-speed atomic force microscopy. Nat. Nanotechnol. 7, 525-529 (2012).
8. Kodera, N., Yamashita, H. & Ando, T. Active damping of the scanner for high-speed atomic force microscopy. Rev. Sci. Instrum. 76, 053708 (2005).
9. Yamashita, H. et al. Single-molecule imaging on living bacterial cell surface by high-speed AFM. J. Mol. Biol. 422, 300-309 (2012).
10. Butt, H. J. et al. Imaging cells with the atomic force microscope. J. Struct. Biol. 105, 54-61 (1990).
11. Madl, J. et al. A combined optical and atomic force microscope for live cell investigations. Ultramicroscopy 106, 645-651 (2006). (Pubitemid 43994308)
12. Lehenkari, P. P., Charras, G. T., Nykänen, A. & Horton, M. A. Adapting atomic force microscopy for cell biology. Ultramicroscopy 82, 289-295 (2000). (Pubitemid 30070113)
13. Jensen, M. O. et al. Dynamic control of slow water transport by aquaporin 0: implications for hydration and junction stability in the eye lens. Proc. Natl Acad. Sci. USA 105, 14430-14435 (2008).
14. Gonen, T. et al. Lipid-protein interactions in double-layered two-dimensional AQP0 crystals. Nature 438, 633-638 (2005). (Pubitemid 41740568)
15. Zampighi, G. A., Hall, J. E., Ehring, G. R. & Simon, S. A. The structural organization and protein composition of lens fiber junctions. J. Cell. Biol. 108, 2255-2275 (1989). (Pubitemid 19168918)
16. Buzhynskyy, N., Girmens, J.-F., Faigle, W. & Scheuring, S. Human cataract lens membrane at subnanometer resolution. J. Mol. Biol. 374, 162-169 (2007). (Pubitemid 47600241)
17. Buzhynskyy, N., Hite, R. K., Walz, T. & Scheuring, S. The supramolecular architecture of junctional microdomains in native lens membranes. EMBO Rep. 8, 51-55 (2007). (Pubitemid 46043800)
18. Colom, A., Casuso, I., Boudier, T. & Scheuring, S. High-speed atomic force microscopy: cooperative adhesion and dynamic equilibrium of junctional microdomain membrane proteins. J. Mol. Biol. 423, 249-256 (2012).
19. Biswas, S. K., Lee, J. E., Brako, L., Jiang, J. X. & Lo, W.-K. Gap junctions are selectively associated with interlocking ball-and-sockets but not protrusions in the lens. Mol. Vis. 16, 2328-2341 (2010).
20. Garc?́a, R. Amplitude Modulation Atomic Force Microscopy 77-90 (Wiley-VCH Verlag GmbH & Co. KGaA, 2010).
21. Husain, M., Boudier, T., Paul-Gilloteaux, P., Casuso, I. & Scheuring, S. Software for drift compensation, particle tracking and particle analysis of high-speed atomic force microscopy image series. J. Mol. Recognit. 25, 292-298 (2012).
22. Engelman, D. M. Membranes are more mosaic than fluid. Nature 438, 578-580 (2005). (Pubitemid 41740560)
23. Simons, K. & Ikonen, E. Functional rafts in cell membranes. Nature 387, 569-572 (1997). (Pubitemid 27248754)
24. Singer, S. J. & Nicolson, G. L. The fluid mosaic model of the structure of cell membranes. Science 175, 720-731 (1972).
25. Schagger, H., Cramer, W. A. & Vonjagow, G. Analysis of molecular masses and oligomeric states of protein complexes by blue native electrophoresis and isolation of membrane protein complexes by two-dimensional native electrophoresis. Anal. Biochem. 217, 220-230 (1994). (Pubitemid 24080754)
26. Marguet, D., Lenne, P. F., Rigneault, H. & He, H. T. Dynamics in the plasma membrane: how to combine fluidity and order. EMBO J. 25, 3446-3457 (2006). (Pubitemid 44264856)
27. Kusumi, A. Paradigm shift of the plasma membrane concept from the twodimensional continuum fluid to the partitioned fluid: high-speed singlemolecule tracking of membrane molecules. Annu. Rev. Biophys. Biomol. Struct. 34, 351-378 (2005). (Pubitemid 40847729)
28. Verkman, A. S., Rossi, A. & Crane, J. M. Methods in Enzymology Vol 504 (Michael Conn, P. ed) 341-354 (Academic Press, 2012).
29. Hozic, A., Rico, F., Colom, A., Buzhynskyy, N. & Scheuring, S. Nanomechanical Characterization of the stiffness of eye lens cells: a pilot study. Invest. Ophthalmol. Vis. Sci. 53, 2151-2156 (2012).