Compartmentalization of destabilized enzyme-mRNA-ribosome complexes generated by ribosome display: A novel tool for the directed evolution of enzymes

Protein Engineering, Design and Selection

Volumn 26, Issue 7, 2013, Pages 453-461

  Skirgaila, Remigijus ^a   Pudzaitis, Vaidas ^b   Paliksa, Sigitas ^a,c   Vaitkevicius, Mindaugas ^a   Janulaitis, Arvydas ^c  

^a Thermo Fisher Scientific   (United States)

^b Bruker Baltic OÜ   (Estonia)

^c VILNIUS UNIVERSITY   (Lithuania)

Author keywords

compartmentalized ribosome display; M MuLV reverse transcriptase; protein in vitro evolution; thermostable reverse transcriptase

Indexed keywords

ENZYMATIC PROPERTIES; IN-VITRO; INCREASED TEMPERATURE; INTERMOLECULAR INTERACTIONS; REVERSE TRANSCRIPTASES; RIBOSOME DISPLAY; SINGLE MOLECULE LEVEL; WATER IN OIL EMULSIONS;

CATALYST ACTIVITY; DROPS; EMULSIONS; MOLECULES; NUCLEIC ACIDS;

ENZYMES;

COMPLEMENTARY DNA; DNA NUCLEOTIDYLEXOTRANSFERASE; DNA POLYMERASE; ENZYME; MESSENGER RNA; MUTANT PROTEIN; POLYDEOXYRIBONUCLEOTIDE SYNTHASE; RNA DIRECTED DNA POLYMERASE; RNA LIGASE; WATER OIL CREAM;

ARTICLE; COMPARTMENTALIZED RIBOSOME DISPLAY; COMPLEX FORMATION; CONTROLLED STUDY; DIRECTED MOLECULAR EVOLUTION; DNA SYNTHESIS; ENZYME ACTIVITY; ENZYME STABILITY; ENZYME SUBSTRATE COMPLEX; IN VITRO STUDY; MICROFLUIDICS; MOLECULAR CLONING; MOLECULAR MODEL; MUTATION; POLYMERASE CHAIN REACTION; PRESSURE; PRIORITY JOURNAL; PROTEIN METABOLISM; RIBOSOME; SEQUENCE ANALYSIS; TEMPERATURE;

COMPARTMENTALIZED RIBOSOME DISPLAY; M-MULV REVERSE TRANSCRIPTASE; PROTEIN IN VITRO EVOLUTION; THERMOSTABLE REVERSE TRANSCRIPTASE;

DIRECTED MOLECULAR EVOLUTION; DNA, COMPLEMENTARY; DNA-DIRECTED DNA POLYMERASE; EMULSIONS; GENE LIBRARY; RIBOSOMES; RNA, MESSENGER; RNA-DIRECTED DNA POLYMERASE;

EID: 84880211903     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzt017     Document Type: Article

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