Excess dNTPs minimize RNA hydrolysis during reverse transcription

BioTechniques

Volumn 33, Issue 5, 2002, Pages 984-990

  Gerard, Gary F ^a   Collins, Sarah ^a   Smith, Michael D ^a  

^a TRANSGENOMIC INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA; DEOXYRIBONUCLEOSIDE TRIPHOSPHATE; MAGNESIUM; MAGNESIUM CHLORIDE; MESSENGER RNA; RNA; RNA DIRECTED DNA POLYMERASE; 2'-DEOXYADENOSINE TRIPHOSPHATE; 2'-DEOXYCYTIDINE 5'-TRIPHOSPHATE; DEOXYADENOSINE PHOSPHATE; DEOXYADENOSINE TRIPHOSPHATE; DEOXYCYTIDINE PHOSPHATE; DEOXYCYTIDINE TRIPHOSPHATE; DEOXYGUANOSINE PHOSPHATE; DEOXYGUANOSINE TRIPHOSPHATE; PYRIMIDINE NUCLEOTIDE; THYMIDINE 5'-TRIPHOSPHATE; THYMIDINE TRIPHOSPHATE; VIRUS PROTEIN;

ARTICLE; CHELATION; CONCENTRATION RESPONSE; DNA SYNTHESIS; HYDROLYSIS; MOLECULAR BIOLOGY; MOLONEY LEUKEMIA ONCOVIRUS; NONHUMAN; REVERSE TRANSCRIPTION; RNA ANALYSIS; RNA STABILITY; TEMPERATURE SENSITIVITY; AGAR GEL ELECTROPHORESIS; AVIAN SARCOMA VIRUS; BIOSYNTHESIS; DNA DENATURATION; DRUG EFFECT; DRUG STABILITY; ENZYMOLOGY; GENETIC TRANSCRIPTION; HALF LIFE TIME; HEAT; METABOLISM;

MOLONEY MURINE LEUKEMIA VIRUS; ONCOVIRINAE;

AVIAN SARCOMA VIRUSES; DEOXYADENINE NUCLEOTIDES; DEOXYCYTOSINE NUCLEOTIDES; DEOXYGUANINE NUCLEOTIDES; DNA, COMPLEMENTARY; DRUG STABILITY; ELECTROPHORESIS, AGAR GEL; HALF-LIFE; HEAT; HYDROLYSIS; MAGNESIUM; MOLONEY MURINE LEUKEMIA VIRUS; NUCLEIC ACID DENATURATION; RNA; RNA-DIRECTED DNA POLYMERASE; THYMINE NUCLEOTIDES; TRANSCRIPTION, GENETIC; VIRAL PROTEINS;

EID: 1842864852     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/02335bm03     Document Type: Article

References (20)

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