Molecular cloning and expression analysis of liver-expressed antimicrobial peptide 1 (LEAP-1) and LEAP-2 genes in the blunt snout bream (Megalobrama amblycephala)

Fish and Shellfish Immunology

Volumn 35, Issue 2, 2013, Pages 553-563

  Liang, Tao ^a   Ji, Wei ^a   Zhang, Gui Rong ^a   Wei, Kai Jian ^a   Feng, Ke ^a   Wang, Wei Min ^a   Zou, Gui Wei ^b  

^a HUAZHONG AGRICULTURAL UNIVERSITY   (China)

^b SOUTH CHINA SEA FISHERIES RESEARCH INSTITUTE   (China)

Author keywords

Antimicrobial peptide; Gene expression; LEAP 1; LEAP 2; Megalobrama amblycephala

Indexed keywords

BACTERIA (MICROORGANISMS); CTENOPHARYNGODON; CYPRINIDAE; HYPEROGLYPHE POROSA; MEGALOBRAMA AMBLYCEPHALA; TELEOSTEI;

EID: 84880037398     PISSN: 10504648     EISSN: 10959947     Source Type: Journal    
DOI: 10.1016/j.fsi.2013.05.021     Document Type: Article

References (46)

1. Magnadóttir B. Innate immunity of fish (overview). Fish Shellfish Immunol 2006, 20:137-151.
2. Zhu L.Y., Nie L., Zhu G., Xiang L.X., Shao J.Z. Advances in research of fish immune-relevant genes: a comparative overview of innate and adaptive immunity in teleosts. Dev Comp Immunol 2013, 39:39-62.
3. Hancock R.E.W., Diamond G. The role of cationic antimicrobial peptides in innate host defences. Trends Microbiol 2000, 8:402-410.
4. Dale B.A., Fredericks L.P. Antimicrobial peptides in the oral environment: expression and function in health and disease. Curr Issues Mol Biol 2005, 7:119.
5. Satchell D.P., Sheynis T., Shirafuji Y., Kolusheva S., Ouellette A.J., Jelinek R. Interactions of mouse paneth cell α-defensins and α-defensin precursors with membranes. JBiol Chem 2003, 278:13838-13846.
6. Townes C.L., Michailidis G., Hall J. The interaction of the antimicrobial peptide cLEAP-2 and the bacterial membrane. Biochem Biophys Res Commun 2009, 387:500-503.
7. Hancock R.E.W. Cationic peptides: effectors in innate immunity and novel antimicrobials. Lancet Infect Dis 2001, 1:156-164.
8. Rajanbabu V., Chen J.-Y. Applications of antimicrobial peptides from fish and perspectives for the future. Peptides 2011, 32:415-420.
9. Krause A., Neitz S., Mägert H.J., Schulz A., Forssmann W.G., Schulz-Knappe P., et al. LEAP-1, a novel highly disulfide-bonded human peptide, exhibits antimicrobial activity. FEBS Lett 2000, 480:147-150.
10. Krause A., Sillard R., Kleemeier B., Kluver E., Maronde E., Conejo-Garcia J.R., et al. Isolation and biochemical characterization of LEAP-2, a novel blood peptide expressed in the liver. Protein Sci 2003, 12:143-152.
11. Park C.H., Valore E.V., Waring A.J., Ganz T. Hepcidin, a urinary antimicrobial peptide synthesized in the liver. JBiol Chem 2001, 276:7806-7810.
12. Shike H., Lauth X., Westerman M.E., Ostland V.E., Carlberg J.M., Van Olst J.C., et al. Bass hepcidin is a novel antimicrobial peptide induced by bacterial challenge. Eur J Biochem 2002, 269:2232-2237.
13. Rajanbabu V., Chen J.Y. Antiviral function of tilapia hepcidin 1-5 and its modulation of immune-related gene expressions against infectious pancreatic necrosis virus (IPNV) in Chinook salmon embryo (CHSE)-214 cells. Fish Shellfish Immunol 2011, 30:39-44.
14. Marguti I. Control of immunopathology during Plasmodium infection by hepcidin. Med Hypotheses 2012, 78:250-253.
15. Nicolas G., Viatte L., Bennoun M., Beaumont C., Kahn A., Vaulont S. Hepcidin, a new iron regulatory peptide. Blood Cells Mol Dis 2002, 29:327-335.
16. Nemeth E., Tuttle M.S., Powelson J., Vaughn M.B., Donovan A., Ward D.M.V., et al. Hepcidin regulates cellular iron efflux by binding to ferroportin and inducing its internalization. Science 2004, 306:2090.
17. Ganz T. Hepcidin - a regulator of intestinal iron absorption and iron recycling by macrophages. Best Pract Res Clin Haematol 2005, 18:171-182.
18. Douglas S.E., Gallant J.W., Liebscher R.S., Dacanay A., Tsoi S.C.M. Identification and expression analysis of hepcidin-like antimicrobial peptides in bony fish. Dev Comp Immunol 2003, 27:589-601.
19. Shike H., Shimizu C., Lauth X., Burns J.C. Organization and expression analysis of the zebrafish hepcidin gene, an antimicrobial peptide gene conserved among vertebrates. Dev Comp Immunol 2004, 28:747-754.
20. Bao B., Peatman E., Li P., He C., Liu Z. Catfish hepcidin gene is expressed in a wide range of tissues and exhibits tissue-specific upregulation after bacterial infection. Dev Comp Immunol 2005, 29:939-950.
21. Chen S.L., Li W., Meng L., Sha Z.X., Wang Z.J., Ren G.C. Molecular cloning and expression analysis of a hepcidin antimicrobial peptide gene from turbot (Scophthalmus maximus). Fish Shellfish Immunol 2007, 22:172-181.
22. Yang M., Chen B., Cai J.J., Peng H., Ling C., Yuan J.J., et al. Molecular characterization of hepcidin AS-hepc2 and AS-hepc6 in black porgy (Acanthopagrus schlegelii): expression pattern responded to bacterial challenge and invitro antimicrobial activity. Comp Biochem Physiol B, Biochem Mol Biol 2011, 158:155-163.
23. Zhou J.G., Wei J.G., Xu D., Cui H.C., Yan Y., Ou-Yang Z.L., et al. Molecular cloning and characterization of two novel hepcidins from orange-spotted grouper, Epinephelus coioides. Fish Shellfish Immunol 2011, 30:559-568.
24. Masso-Silva J., Diamond G., Macias-Rodriguez M., Ascencio F. Genomic organization and tissue-specific expression of hepcidin in the pacific mutton hamlet, Alphestes immaculatus (Breder, 1936). Fish Shellfish Immunol 2011, 31:1297-1302.
25. Wang Y., Liu X., Ma L., Yu Y., Yu H., Mohammed S., et al. Identification and characterization of a hepcidin from half-smooth tongue sole Cynoglossus semilaevis. Fish Shellfish Immunol 2012, 33:213-219.
26. Zhang J., Yan Q., Ji R., Zou W., Guo G. Isolation and characterization of a hepcidin peptide from the head kidney of large yellow croaker, Pseudosciaena crocea. Fish Shellfish Immunol 2009, 26:864-870.
27. Zhang Y.A., Zou J., Chang C.I., Secombes C.J. Discovery and characterization of two types of liver-expressed antimicrobial peptide 2 (LEAP-2) genes in rainbow trout. Vet Immunol Immunopathol 2004, 101:259-269.
28. Bao B., Peatman E., Xu P., Li P., Zeng H., He C., et al. The catfish liver-expressed antimicrobial peptide 2 (LEAP-2) gene is expressed in a wide range of tissues and developmentally regulated. Mol Immunol 2006, 43:367-377.
29. Liu F., Li J.L., Yue G.H., Fu J.J., Zhou Z.F. Molecular cloning and expression analysis of the liver-expressed antimicrobial peptide 2 (LEAP-2) gene in grass carp. Vet Immunol Immunopathol 2010, 133:133-143.
30. Li S.F., Cai W.Q., Zhou B.Y. Morphology and biochemical genetic vatiations among populations of blunt snout bream (Megalobrama anblycephala). JFish China 1991, 15(3):204-211. [In Chinese].
31. Ko H.W. An excellent fresh-water food fish, Megalobrama amblycephala, and its propagating and culturing. Acta Hydrobiol Sin 1975, 5:293-312. [In Chinese].
32. Fisheries Bureau of Agriculrture Ministry of China (FBAM) China fishery statistical yearbook 2011, Chinese Agricultural Press, Beijing, [In Chinese].
33. Li S.F., Cai W.Q. Genetic improvement of the herbivorous blunt snout bream (Megalobrama amblycephala). Naga, WorldFish Cent Q 2003, 26(1):20-23.
34. Yu P.C., Zhang F.W. Observations on the embryonic development of Megalobrama amblycephala in the fishing areas of Poyang Lake. JFish Sci China 1998, 5(1):103-108. [In Chinese].
35. Tian T., Hu H.G., Chen C.F. Identification and pathogenicity of bacterial pathogens isolated in an outbreak of bacterial disease of bluntsnose black bream, Megalobrama amblycephala. JHuazhong Agric Univ 2010, 29:341-345. [In Chinese].
36. Nam B.-H., Moon J.-Y., Kim Y.-O., Kong H.J., Kim W.-J., Lee S.-J., et al. Multiple β-defensin isoforms identified in early developmental stages of the teleost Paralichthys olivaceus. Fish Shellfish Immunol 2010, 28:267-274.
37. Broekman D.C., Frei D.M., Gylfason G.A., Steinarsson A., Jörnvall H., Agerberth B., et al. Cod cathelicidin: isolation of the mature peptide, cleavage site characterisation and developmental expression. Dev Comp Immunol 2011, 35:296-303.
38. Sang Y., Ramanathan B., Minton J.E., Ross C.R., Blecha F. Porcine liver-expressed antimicrobial peptides, hepcidin and LEAP-2: cloning and induction by bacterial infection. Dev Comp Immunol 2006, 30:357-366.
39. Kim Y.O., Park E.M., Nam B.H., Kong H.J., Kim W.J., Lee S.J. Identification and molecular characterization of two hepcidin genes from black rockfish (Sebastes schlegelii). Mol Cell Biochem 2008, 315:131-136.
40. Badial P.R., Oliveira Filho J.P., Cunha P.H., Cagnini D.Q., Araujo J.P., Winand N.J., et al. Identification, characterization and expression analysis of hepcidin gene in sheep. Res Vet Sci 2011, 90:443-450.
41. Nam Y.K., Cho Y.S., Lee S.Y., Kim B.S., Kim D.S. Molecular characterization of hepcidin gene from mud loach (Misgurnus mizolepis; Cypriniformes). Fish Shellfish Immunol 2011, 31:1251-1258.
42. Cuesta A., Meseguer J., Esteban M.A. The antimicrobial peptide hepcidin exerts an important role in the innate immunity against bacteria in the bony fish gilthead seabream. Mol Immunol 2008, 45:2333-2342.
43. Chen S.L., Xu M.Y., Ji X.S., Yu G.C., Liu Y. Cloning, characterization, and expression analysis of hepcidin gene from red sea bream (Chrysophrys major). Antimicrob Agents Chemother 2005, 49:1608-1612.
44. Hu X., Camus A.C., Aono S., Morrison E.E., Dennis J., Nusbaum K.E., et al. Channel catfish hepcidin expression in infection and anemia. Comp Immunol Microbiol Infect Dis 2007, 30:55-69.
45. Guo M.L., Wei J.G., Huang X.H., Huang Y.H., Qin Q.W. Antiviral effects of β-defensin derived from orange-spotted grouper (Epinephelus coioides). Fish Shellfish Immunol 2012, 32:828-838.
46. Lu X.J., Chen J., Huang Z.A., Shi Y.H., Lü J.N. Identification and characterization of a novel cathelicidin from ayu, Plecoglossus altivelis. Fish Shellfish Immunol 2011, 31:52-57.