Cod cathelicidin: Isolation of the mature peptide, cleavage site characterisation and developmental expression

Developmental and Comparative Immunology

Volumn 35, Issue 3, 2011, Pages 296-303

  Broekman, Daniela C ^a   Frei, Dominik M ^a,d   Gylfason, Gudjon A ^a   Steinarsson, Agnar ^b   Jörnvall, Hans ^c   Agerberth, Birgitta ^c   Gudmundsson, Gudmundur H ^a   Maier, Valerie H ^a  

^a UNIVERSITY OF ICELAND   (Iceland)

^b MARINE RESEARCH INSTITUTE   (Iceland)

^c KAROLINSKA INSTITUTE   (Sweden)

^d UNIVERSITY OF FREIBURG   (Germany)

Author keywords

Antimicrobial peptide; Atlantic cod; Fish immunity; Innate immunity; Peptide purification

Indexed keywords

CATHELICIDIN;

AMINO ACID SEQUENCE; ANIMAL EXPERIMENT; ANIMAL TISSUE; ANTIMICROBIAL ACTIVITY; ARTICLE; ATLANTIC COD; CONTROLLED STUDY; FOOD INTAKE; HATCHING; IMMUNE SYSTEM; LARVAL DEVELOPMENT; NONHUMAN; PEPTIDE SYNTHESIS; PRIORITY JOURNAL; PROTEIN CLEAVAGE; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN ISOLATION; REAL TIME POLYMERASE CHAIN REACTION; STIMULUS RESPONSE;

GADUS MORHUA; MAMMALIA; TELEOSTEI;

EID: 78751645533     PISSN: 0145305X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.dci.2010.10.002     Document Type: Article

References (38)

1. Bergman P., Termen S., Johansson L., Nystrom L., Arenas E., Jonsson A.B., Hokfelt T., Gudmundsson G.H., Agerberth B. The antimicrobial peptide rCRAMP is present in the central nervous system of the rat. J. Neurochem. 2005, 93:1132-1140.
2. Bergsson G., Agerberth B., Jornvall H., Gudmundsson G.H. Isolation and identification of antimicrobial components from the epidermal mucus of Atlantic cod (Gadus morhua). FEBS J. 2005, 272:4960-4969.
3. Chang C.I., Pleguezuelos O., Zhang Y.A., Zou J., Secombes C.J. Identification of a novel cathelicidin gene in the rainbow trout, Oncorhynchus mykiss. Infect. Immun. 2005, 73:5053-5064.
4. Chang C.I., Zhang Y.A., Zou J., Nie P., Secombes C.J. Two cathelicidin genes are present in both rainbow trout (Oncorhynchus mykiss) and atlantic salmon (Salmo salar). Antimicrob. Agents Chemother. 2006, 50:185-195.
5. Corrales J., Gordon W.L., Noga E.J. Development of an ELISA for quantification of the antimicrobial peptide piscidin 4 and its application to assess stress in fish. Fish Shellfish Immunol. 2009, 27:154-163.
6. de Sousa Abreu R., Penalva L.O., Marcotte E.M., Vogel C. Global signatures of protein and mRNA expression levels. Mol. Biosyst. 2009, 5:1512-1526.
7. Gudmundsdottir B.K., Bjornsdottir B. Vaccination against atypical furunculosis and winter ulcer disease of fish. Vaccine 2007, 25:5512-5523.
8. Lai Y., Gallo R.L. AMPed up immunity: how antimicrobial peptides have multiple roles in immune defense. Trends Immunol. 2009, 30:131-141.
9. Livak K.J., Schmittgen T.D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-ΔΔC(T)) method. Methods 2001, 25:402-408.
10. Magnadottir B. Immunological control of fish diseases. Mar. Biotechnol. (NY) 2010, 12:361-379.
11. Magnadottir B., Gudmundsdottir S., Gudmundsdottir B.K., Helgason S. Natural antibodies of cod (Gadus morhua L.): specificity, activity and affinity. Comp. Biochem. Physiol. B Biochem. Mol. Biol. 2009, 154:309-316.
12. Magnadottir B., Lange S., Steinarsson A., Gudmundsdottir S. The ontogenic development of innate immune parameters of cod (Gadus morhua L.). Comp. Biochem. Physiol. B Biochem. Mol. Biol. 2004, 139:217-224.
13. Maier V.H., Dorn K.V., Gudmundsdottir B.K., Gudmundsson G.H. Characterisation of cathelicidin gene family members in divergent fish species. Mol. Immunol. 2008, 45:3723-3730.
14. Maier V.H., Schmitt C.N., Gudmundsdottir S., Gudmundsson G.H. Bacterial DNA indicated as an important inducer of fish cathelicidins. Mol. Immunol. 2008, 45:2352-2358.
15. Marshall O.J. PerlPrimer: cross-platform, graphical primer design for standard, bisulphite and real-time PCR. Bioinformatics 2004, 20:2471-2472.
16. Murakami M., Lopez-Garcia B., Braff M., Dorschner R.A., Gallo R.L. Postsecretory processing generates multiple cathelicidins for enhanced topical antimicrobial defense. J. Immunol. 2004, 172:3070-3077.
17. Nakayama K. Furin: a mammalian subtilisin/Kex2p-like endoprotease involved in processing of a wide variety of precursor proteins. Biochem. J. 1997, 327(Pt 3):625-635.
18. Ogiwara K., Shinohara M., Takahashi T. Structure and expression of Furin mRNA in the ovary of the medaka, Oryzias latipes. J. Exp. Zool. A Comp. Exp. Biol. 2004, 301:449-459.
19. Olafsen J.A. Interactions between fish larvae and bacteria in marine aquaculture. Aquaculture 2001, 200:223-247.
20. Olsvik P.A., Softeland L., Lie K.K. Selection of reference genes for qRT-PCR examination of wild populations of Atlantic cod Gadus morhua. BMC Res. Notes 2008, 1:47.
21. Panyutich A., Shi J., Boutz P.L., Zhao C., Ganz T. Porcine polymorphonuclear leukocytes generate extracellular microbicidal activity by elastase-mediated activation of secreted proprotegrins. Infect. Immun. 1997, 65:978-985.
22. Patat S.A., Carnegie R.B., Kingsbury C., Gross P.S., Chapman R., Schey K.L. Antimicrobial activity of histones from hemocytes of the Pacific white shrimp. Eur. J. Biochem. 2004, 271:4825-4833.
23. Perez T., Balcazar J.L., Ruiz-Zarzuela I., Halaihel N., Vendrell D., de Blas I., Muzquiz J.L. Host-microbiota interactions within the fish intestinal ecosystem. Mucosal. Immunol. 2010, 3:355-360.
24. Saele O., Nordgreen A., Hamre K., Olsvik P.A. Evaluation of candidate reference genes in Q-PCR studies of Atlantic cod (Gadus morhua) ontogeny, with emphasis on the gastrointestinal tract. Comp. Biochem. Physiol. B Biochem. Mol. Biol. 2009, 152:94-101.
25. Schroder M.B., Villena A.J., Jorgensen T.O. Ontogeny of lymphoid organs and immunoglobulin producing cells in Atlantic cod (Gadus morhua L.). Dev. Comp. Immunol. 1998, 22:507-517.
26. Scocchi M., Pallavicini A., Salgaro R., Bociek K., Gennaro R. The salmonid cathelicidins: a gene family with highly varied C-terminal antimicrobial domains. Comp. Biochem. Physiol. B Biochem. Mol. Biol. 2009, 152:376-381.
27. Scocchi M., Skerlavaj B., Romeo D., Gennaro R. Proteolytic cleavage by neutrophil elastase converts inactive storage proforms to antibacterial bactenecins. Eur. J. Biochem. 1992, 209:589-595.
28. Seppola M., Johnsen H., Mennen S., Myrnes B., Tveiten H. Maternal transfer and transcriptional onset of immune genes during ontogenesis in Atlantic cod. Dev. Comp. Immunol. 2009, 33:1205-1211.
29. Sorensen O.E., Follin P., Johnsen A.H., Calafat J., Tjabringa G.S., Hiemstra P.S., Borregaard N. Human cathelicidin, hCAP-18, is processed to the antimicrobial peptide LL-37 by extracellular cleavage with proteinase 3. Blood 2001, 97:3951-3959.
30. Steinarsson A. Hatchery production of cod. Cod Farming in Iceland 2004, Marine Research Institute. B. Bjornsson, V.I. Gunnarsson (Eds.).
31. Tomasinsig L., Zanetti M. The cathelicidins-structure, function and evolution. Curr. Protein Pept. Sci. 2005, 6:23-34.
32. Uzzell T., Stolzenberg E.D., Shinnar A.E., Zasloff M. Hagfish intestinal antimicrobial peptides are ancient cathelicidins. Peptides 2003, 24:1655-1667.
33. van Dijk A., Veldhuizen E.J., Kalkhove S.I., Tjeerdsma-van Bokhoven J.L., Romijn R.A., Haagsman H.P. The beta-defensin gallinacin-6 is expressed in the chicken digestive tract and has antimicrobial activity against food-borne pathogens. Antimicrob. Agents Chemother. 2007, 51:912-922.
34. Walker M.B., Miller C.T., Coffin Talbot J., Stock D.W., Kimmel C.B. Zebrafish furin mutants reveal intricacies in regulating Endothelin 1 signaling in craniofacial patterning. Dev. Biol. 2006, 295:194-205.
35. Zasloff M. Antimicrobial peptides of multicellular organisms. Nature 2002, 415:389-395.
36. Zelezetsky I., Pontillo A., Puzzi L., Antcheva N., Segat L., Pacor S., Crovella S., Tossi A. Evolution of the primate cathelicidin. Correlation between structural variations and antimicrobial activity. J. Biol. Chem. 2006, 281:19861-19871.
37. Zhu S. Did cathelicidins, a family of multifunctional host-defense peptides, arise from a cysteine protease inhibitor?. Trends Microbiol. 2008, 16:353-360.
38. Zhu S., Gao B. A fossil antibacterial peptide gives clues to structural diversity of cathelicidin-derived host defense peptides. FASEB J. 2009, 23:13-20.