Variants of DsRed fluorescent protein: Development of a copper sensor

Protein Science

Volumn 15, Issue 10, 2006, Pages 2442-2447

  Eli, Pharhad ^a   Chakrabartty, Avijit ^a  

^a UNIVERSITY OF TORONTO   (Canada)

Author keywords

Copper ion; drfp583, DsRed; Fluorescence quenching; Random mutagenesis; Red fluorescent protein

Indexed keywords

COPPER ION; COPPER SULFATE; GREEN FLUORESCENT PROTEIN; PROTEIN; PROTEIN DRFP583; RED FLUORESCENT PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; FLUORESCENCE; METAL BINDING; MUTAGENESIS; PH; PRIORITY JOURNAL; PROTEIN INTERACTION; PROTEIN PURIFICATION; PROTEIN VARIANT; SPECTROSCOPY;

BIOSENSING TECHNIQUES; COPPER; COPPER SULFATE; HYDROGEN-ION CONCENTRATION; LUMINESCENT PROTEINS; MUTAGENESIS; PROTEIN BINDING; SPECTROMETRY, FLUORESCENCE;

EID: 33749318823     PISSN: 09618368     EISSN: 1469896X     Source Type: Journal    
DOI: 10.1110/ps.062239206     Document Type: Article

References (20)

1. Atwood, C.S., Moir, R.D., Huang, X., Scarpa, R.C., Bacarra, N.M.E., Romano, D.M., Hartshorn, M.A., Rudolph, R.T., and Bush, A.I. 1998. Dramatic aggregation of Alzheimer Ab by Cu(II) is induced by conditions representing physiological acidosis. J. Biol. Chem. 273: 12817-12826.
2. Atwood, C.S., Scarpa, R.C., Huang, X., Moir, R.D., Jones, W.D., Fairlie, D.P., Anzi, R.E., and Bush, A.I. 2000. Characterization of copper interactions with Alzheimer amyloid β peptides. Identification of an attomolar-affinity copper binding site on amyloid β1-42. J. Neurochem. 75: 1219-1233.
3. Baird, G.S., Zacharias, D.A., and Tsien, R.Y. 2000. Biochemical, mutagenesis, and oligomerization of DsRed, a red fluorescent protein from coral. Proc. Natl. Acad. Sci. 97: 11984-11989.
4. Brown, D.R., Qin, K., Herms, J.W., Madlung, A., Manson, J., Strome, R., Fraser, P.E., Kruck, T., Bohlen, A., Schulz-Schaeffer, W., et al. 1997. The cellular prion protein binds copper in vivo. Nature 390: 684-687.
5. Cadwell, R.C. and Joyce, G.F. 1994. Mutagenic PCR. PCR Methods Appl. 3: S136-S140.
6. Cherny, R.A., Atwood, C.S., Xilinas, M.E., Gray, D.N., Jones, W.D., McLean, C.A., Barnham, K.J., Volitakis, I., Fraser, F.W., Kim, Y.S., et al. 2001. Treatment with a copper-zinc chelator markedly and rapidly inhibits β-amyloid accumulation in Alzheimer's disease transgenic mice. Neuron 30: 665-676.
7. Jensen, K.K., Martini, L., and Schwartz, T.W. 2001. Enhanced fluorescence energy transfer between spectral variants of green fluorescent protein through zinc-site engineering. Biochemistry 40: 938-945.
8. Matz, M.V., Fradkov, A.F., Labas, Y.A., Savitsky, A.P., Zaraisky, A.G., Markelov, M.L., and Lukyanov, S.A. 1999. Fluorescent proteins from nonbioluminescent Anthozao species. Nat. Biotechnol. 17: 969-973.
9. Mizuno, H., Sawano, A., Eli, P., Hama, H., and Miyawaki, A. 2001. Red fluorescent protein from discosoma as a fusion tag and a partner for fluorescence resonance energy transfer. Biochemistry 40: 2502-2510.
10. Prasher, D.C., Eckenrode, V.K., Ward, W.W., Prendergast, F.G., and Cormier, M.J. 1992. Primary structure of the Aequorea victoria green-fluorescent protein. Gene 111: 229-233.
11. Qin, K., Yang, Y., Mastrangelo, P., and Westaway, D. 2002. Mapping Cu(II) binding sites in prion proteins by diethyl pyrocarbonate modification and matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometric footprinting. J. Biol. Chem. 277: 1981-1990.
12. Qin, K., Coomaraswamy, J., Mastrangelo, P., Yang, Y., Lugowski, S., Petromilli, C., Prusiner, S.B., Fraser, P.E., Goldberg, J.M., Chakrabartty, A., et al. 2003. The PrP-like protein Doppel binds copper. J. Biol. Chem. 278: 8888-8896.
13. Rakhit, R., Cunningham, P., Furtos-Matei, A., Dahan, S., Qi, X.F., Crow, J.P., Cashman, N.R., Kondejewski, L.H., and Chakrabartty, A. 2002. Oxidation-induced misfolding and aggregation of superoxide dismutase and its implications for amyotrophic lateral sclerosis. J. Biol. Chem. 277: 47551-47556.
14. Richmond, T.A., Takahashi, T.T., Shimkhada, R., and Bernsdorf, J. 2000. Engineered metal biding sites on green fluorescence protein. Biochem. Biophys. Res. Commun. 268: 462-465.
15. Sawano, A. and Miyawaki, A. 2000. Directed evolution of green fluorescent protein by a new versatile PCR strategy for site-directed and semi-random mutagenesis. Nucleic Acids Res. 28: e78.
16. 2+ ions. Biosens. Bioelectron. 21: 1302-1308.
17. Verkhusha, V.V., Otsuna, H., Awasaki, T., Oda, H., Tsukita, S., and Ito, K. 2001. An enhanced mutant of red fluorescent protein DsRed for double labeling and developmental timer of neural fiber bundle formation. J. Biol. Chem. 276: 29621-29624.
18. Vrzheshch, P.V., Akovbian, N.A., Varfolomeyev, S.D., and Verkhusha, V.V. 2000. Denaturation and partial renaturation of a tightly tetramerized DsRed protein under mildly acidic conditions. FEBS Lett. 487: 203-208.
19. Wall, M.A., Socolich, M., and Ranganathan, R. 2000. The structural basis for red fluorescence in the tetrameric GFP homolog DsRed. Nat. Struct. Biol. 7: 1133-1138.
20. Yarbrough, D., Wachter, R.M., Kallio, K., Maltz, M.V., and Remington, S.J. 2001. Refined crystal structure of DsRed, a red fluorescent protein from coral, at 2.0-Å resolution. Proc. Natl. Acad. Sci. 98: 462-467.