Development and evaluation of a real-time PCR assay for detection of Pneumocystis jirovecii on the fully automated BD MAX platform

Journal of Clinical Microbiology

Volumn 51, Issue 7, 2013, Pages 2337-2343

  Dalpke, Alexander H ^a   Hofko, Marjeta ^a   Zimmermann, Stefan ^a  

^a UNIVERSITY HOSPITAL HEIDELBERG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

COTRIMOXAZOLE;

ARTICLE; AUTOMATION; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; DIAGNOSTIC TEST ACCURACY STUDY; DNA EXTRACTION; FUNGAL DETECTION; HUMAN; INTERMETHOD COMPARISON; LIMIT OF DETECTION; MAJOR CLINICAL STUDY; MEDICAL DEVICE; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; NUCLEOTIDE SEQUENCE; PNEUMOCYSTIS JIROVECI; PNEUMOCYSTIS PNEUMONIA; PRIORITY JOURNAL; PROCESS OPTIMIZATION; PROSPECTIVE STUDY; REAL TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY; RETROSPECTIVE STUDY; SENSITIVITY AND SPECIFICITY; VALIDITY; EVALUATION STUDY; GENETICS; ISOLATION AND PURIFICATION; LABORATORY AUTOMATION; MICROBIOLOGICAL EXAMINATION; MICROBIOLOGY; MOLECULAR DIAGNOSIS; PNEUMOCYSTIS INFECTIONS; PROCEDURES;

PNEUMOCYSTIS JIROVECII;

AUTOMATION, LABORATORY; HUMANS; MICROBIOLOGICAL TECHNIQUES; MOLECULAR DIAGNOSTIC TECHNIQUES; PNEUMOCYSTIS INFECTIONS; PNEUMOCYSTIS JIROVECII; PROSPECTIVE STUDIES; REAL-TIME POLYMERASE CHAIN REACTION; RETROSPECTIVE STUDIES; SENSITIVITY AND SPECIFICITY;

EID: 84879428526     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.00616-13     Document Type: Article

References (32)

1. Thomas CF, Jr, Limper AH. 2004. Pneumocystis pneumonia. N. Engl. J. Med. 350:2487-2498.
2. Sowden E, Carmichael AJ. 2004. Autoimmune inflammatory disorders, systemic corticosteroids and pneumocystis pneumonia: a strategy for prevention. BMC Infect. Dis. 4:42.
3. Li MC, Lee NY, Lee CC, Lee HC, Chang CM, Ko WC. 10 October 2012. Pneumocystis jiroveci pneumonia in immunocompromised patients: delayed diagnosis and poor outcomes in non-HIV-infected individuals. J. Microbiol. Immunol. Infect. doi:10.1016/j.jmii.2012.08.024.
4. Roblot F, Godet C, Le Moal G, Garo B, Faouzi Souala M, Dary M, De Gentile L, Gandji JA, Guimard Y, Lacroix C, Roblot P, Becq-Giraudon B. 2002. Analysis of underlying diseases and prognosis factors associated with Pneumocystis carinii pneumonia in immunocompromised HIVnegative patients. Eur. J. Clin. Microbiol. Infect. Dis. 21:523-531.
5. Kaiser K, Rabodonirina M, Picot S. 2001. Real time quantitative PCR and RT-PCR for analysis of Pneumocystis carinii hominis. J. Microbiol. Methods 45:113-118.
6. Palladino S, Kay I, Fonte R, Flexman J. 2001. Use of real-time PCR and the LightCycler system for the rapid detection of Pneumocystis carinii in respiratory specimens. Diagn. Microbiol. Infect. Dis. 39:233-236.
7. Larsen HH, Masur H, Kovacs JA, Gill VJ, Silcott VA, Kogulan P, Maenza J, Smith M, Lucey DR, Fischer SH. 2002. Development and evaluation of a quantitative, touch-down, real-time PCR assay for diagnosing Pneumocystis carinii pneumonia. J. Clin. Microbiol. 40:490-494.
8. Meliani L, Develoux M, Marteau-Miltgen M, Magne D, Barbu V, Poirot JL, Roux P. 2003. Real time quantitative PCR assay for Pneumocystis jirovecii detection. J. Eukaryot. Microbiol. 50(Suppl):651.
9. Flori P, Bellete B, Durand F, Raberin H, Cazorla C, Hafid J, Lucht F, Sung RT. 2004. Comparison between real-time PCR, conventional PCR and different staining techniques for diagnosing Pneumocystis jiroveci pneumonia from bronchoalveolar lavage specimens. J. Med. Microbiol. 53:603-607.
10. Brancart F, Rodriguez-Villalobos H, Fonteyne PA, Peres-Bota D, Liesnard C. 2005. Quantitative TaqMan PCR for detection of Pneumocystis jiroveci. J. Microbiol. Methods 61:381-387.
11. Strutt M, Smith M. 2005. Development of a real-time probe-based PCR assay for the diagnosis of Pneumocystis pneumonia. Med. Mycol. 43:343-347.
12. Arcenas RC, Uhl JR, Buckwalter SP, Limper AH, Crino D, Roberts GD, Wengenack NL. 2006. A real-time polymerase chain reaction assay for detection of Pneumocystis from bronchoalveolar lavage fluid. Diagn. Microbiol. Infect. Dis. 54:169-175.
13. Robberts FJ, Liebowitz LD, Chalkley LJ. 2007. Polymerase chain reaction detection of Pneumocystis jiroveci: evaluation of 9 assays. Diagn. Microbiol. Infect. Dis. 58:385-392.
14. Bandt D, Monecke S. 2007. Development and evaluation of a real-time PCR assay for detection of Pneumocystis jiroveci. Transpl. Infect. Dis. 9:196-202.
15. Huggett JF, Taylor MS, Kocjan G, Evans HE, Morris-Jones S, Gant V, Novak T, Costello AM, Zumla A, Miller RF. 2008. Development and evaluation of a real-time PCR assay for detection of Pneumocystis jirovecii DNA in bronchoalveolar lavage fluid of HIV-infected patients. Thorax 63:154-159.
16. Rohner P, Jacomo V, Studer R, Schrenzel J, Graf JD. 2009. Detection of Pneumocystis jirovecii by two staining methods and two quantitative PCR assays. Infection 37:261-265.
17. Fujisawa T, Suda T, Matsuda H, Inui N, Nakamura Y, Sato J, Toyoshima M, Nakano Y, Yasuda K, Gemma H, Hayakawa H, Chida K. 2009. Real-time PCR is more specific than conventional PCR for induced sputum diagnosis of Pneumocystis pneumonia in immunocompromised patients without HIV infection. Respirology 14:203-209.
18. Wilson JW, Limper AH, Grys TE, Karre T, Wengenack NL, Binnicker MJ. 2011. Pneumocystis jirovecii testing by real-time polymerase chain reaction and direct examination among immunocompetent and immunosuppressed patient groups and correlation to disease specificity. Diagn. Microbiol. Infect. Dis. 69:145-152.
19. Orsi CF, Gennari W, Venturelli C, La Regina A, Pecorari M, Righi E, Machetti M, Blasi E. 2012. Performance of 2 commercial real-time polymerase chain reaction assays for the detection of Aspergillus and Pneumocystis DNA in bronchoalveolar lavage fluid samples from critical care patients. Diagn. Microbiol. Infect. Dis. 73:138-143.
20. Harris JR, Marston BJ, Sangrujee N, DuPlessis D, Park B. 2011. Costeffectiveness analysis of diagnostic options for pneumocystis pneumonia (PCP). PLoS One 6:e23158. doi:10.1371/journal.pone.0023158.
21. Peterson JC, Cushion MT. 2005. Pneumocystis: not just pneumonia. Curr. Opin. Microbiol. 8:393-398.
22. Dalpke AH, Hofko M, Zimmermann S. 2012. Comparison of the BD Max methicillin-resistant Staphylococcus aureus (MRSA) assay and the BD GeneOhm MRSA achromopeptidase assay with direct-and enrichedculture techniques using clinical specimens for detection of MRSA. J. Clin. Microbiol. 50:3365-3367.
23. Le Guern R, Herwegh S, Grandbastien B, Courcol R, Wallet F. 2012. Evaluation of a new molecular test, the BD Max Cdiff, for detection of toxigenic Clostridium difficile in fecal samples. J. Clin. Microbiol. 50: 3089-3090.
24. Riedlinger J, Beqaj SH, Milish MA, Young S, Smith R, Dodd M, Hankerd RE, Lebar WD, Newton DW. 2010. Multicenter evaluation of the BD Max GBS assay for detection of group B streptococci in prenatal vaginal and rectal screening swab specimens from pregnant women. J. Clin. Microbiol. 48:4239-4241.
25. Botterel F, Cabaret O, Foulet F, Cordonnier C, Costa JM, Bretagne S. 2012. Clinical significance of quantifying Pneumocystis jirovecii DNA by using real-time PCR in bronchoalveolar lavage fluid from immunocompromised patients. J. Clin. Microbiol. 50:227-231.
26. Rodriguez M, Fishman JA. 2004. Prevention of infection due to Pneumocystis spp. in human immunodeficiency virus-negative immunocompromised patients. Clin. Microbiol. Rev. 17:770-782.
27. Limper AH, Offord KP, Smith TF, Martin WJ, II. 1989. Pneumocystis carinii pneumonia. Differences in lung parasite number and inflammation in patients with and without AIDS. Am. Rev. Respir. Dis. 140:1204-1209.
28. Fillaux J, Malvy S, Alvarez M, Fabre R, Cassaing S, Marchou B, Linas MD, Berry A. 2008. Accuracy of a routine real-time PCR assay for the diagnosis of Pneumocystis jirovecii pneumonia. J. Microbiol. Methods 75:258-261.
29. Muhlethaler K, Bogli-Stuber K, Wasmer S, von Garnier C, Dumont P, Rauch A, Muhlemann K, Garzoni C. 2012. Quantitative PCR to diagnose Pneumocystis pneumonia in immunocompromised non-HIV patients. Eur. Respir. J. 39:971-978.
30. Alanio A, Desoubeaux G, Sarfati C, Hamane S, Bergeron A, Azoulay E, Molina JM, Derouin F, Menotti J. 2011. Real-time PCR assay-based strategy for differentiation between active Pneumocystis jirovecii pneumonia and colonization in immunocompromised patients. Clin. Microbiol. Infect. 17:1531-1537.
31. Hauser PM, Bille J, Lass-Florl C, Geltner C, Feldmesser M, Levi M, Patel H, Muggia V, Alexander B, Hughes M, Follett SA, Cui X, Leung F, Morgan G, Moody A, Perlin DS, Denning DW. 2011. Multicenter, prospective clinical evaluation of respiratory samples from subjects at risk for Pneumocystis jirovecii infection by use of a commercial real-time PCR assay. J. Clin. Microbiol. 49:1872-1878.
32. Fillaux J, Berry A. 2013. Real-time PCR assay for the diagnosis of Pneumocystis jirovecii pneumonia. Methods Mol. Biol. 943:159-170.