Quantitative PCR to diagnose Pneumocystis pneumonia in immunocompromised non-HIV patients

European Respiratory Journal

Volumn 39, Issue 4, 2012, Pages 971-978

  Muhlethaler K ^a   Bogli Stuber K ^a   Wasmer, S ^a   Von Garnier, C ^b   Dumont, P ^b   Rauch, A ^a   Muhlemann K ^a   Garzoni, C ^a  

^a UNIVERSITY OF BERN   (Switzerland)

^b UNIVERSITY OF BERN   (Switzerland)

Author keywords

HIV negative; Immunocompromised; Molecular diagnosis; Pneumocystis jirovecii; Pneumocystis pneumonia; Quantitative PCR

Indexed keywords

ADOLESCENT; AGED; ARTICLE; CLINICAL ASSESSMENT; CLINICAL DECISION MAKING; CONTROLLED STUDY; DIAGNOSTIC TEST ACCURACY STUDY; DIAGNOSTIC VALUE; DISEASE ASSOCIATION; FEMALE; FUNGAL COLONIZATION; FUNGUS IDENTIFICATION; HUMAN; IMMUNOCOMPROMISED PATIENT; IMMUNOFLUORESCENCE TEST; LUNG LAVAGE; MAJOR CLINICAL STUDY; MALE; MOLECULAR DIAGNOSIS; PNEUMOCYSTIS JIROVECI; PNEUMOCYSTIS PNEUMONIA; PREDICTION; PREDICTIVE VALUE; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; RELIABILITY; SYNDROME DELINEATION;

ADOLESCENT; ADULT; AGED; AGED, 80 AND OVER; ALGORITHMS; BRONCHOALVEOLAR LAVAGE FLUID; FEMALE; HUMANS; IMMUNOCOMPROMISED HOST; MALE; MIDDLE AGED; OPPORTUNISTIC INFECTIONS; PNEUMOCYSTIS JIROVECII; PNEUMONIA, PNEUMOCYSTIS; REAL-TIME POLYMERASE CHAIN REACTION; RETROSPECTIVE STUDIES; YOUNG ADULT;

EID: 84860325038     PISSN: 09031936     EISSN: 13993003     Source Type: Journal    
DOI: 10.1183/09031936.00095811     Document Type: Article

References (31)

1. Kovacs JA, Masur H. Evolving health effects of Pneumocystis: one hundred years of progress in diagnosis and treatment. JAMA 2009; 301: 2578-2585.
2. Thomas CF Jr, Limper AH. Pneumocystis pneumonia. N Engl J Med 2004; 350: 2487-2498.
3. Bollee G, Sarfati C, Thiery G, et al. Clinical picture of Pneumocystis jiroveci pneumonia in cancer patients. Chest 2007; 132: 1305-1310. (Pubitemid 47621008)
4. Sepkowitz KA. Pneumocystis carinii pneumonia in patients without AIDS. Clin Infect Dis 1993; 17: Suppl. 2, S416-S422. (Pubitemid 23322834)
5. Yale SH, Limper AH. Pneumocystis carinii pneumonia in patients without acquired immunodeficiency syndrome: associated illness and prior corticosteroid therapy. Mayo Clin Proc 1996; 71: 5-13. (Pubitemid 26021784)
6. Ward MM, Donald F. Pneumocystis carinii pneumonia in patients with connective tissue diseases: the role of hospital experience in diagnosis and mortality. Arthritis Rheum 1999; 42: 780-789. (Pubitemid 29172000)
7. Martin SI, Fishman JA. Pneumocystis pneumonia in solid organ transplant recipients. Am J Transplant 2009; 9: Suppl. 4, S227-S233.
8. Nuesch R, Bellini C, Zimmerli W. Pneumocystis carinii pneumonia in human immunodeficiency virus (HIV)-positive and HIVnegative immunocompromised patients. Clin Infect Dis 1999; 29: 1519-1523. (Pubitemid 30022751)
9. Kovacs JA, Hiemenz JW, Macher AM, et al. Pneumocystis carinii pneumonia: a comparison between patients with the acquired immunodeficiency syndrome and patients with other immunodeficiencies. Ann Intern Med 1984; 100: 663-671. (Pubitemid 14143939)
10. Procop GW, Haddad S, Quinn J, et al. Detection of Pneumocystis jiroveci in respiratory specimens by four staining methods. J Clin Microbiol 2004; 42: 3333-3335. (Pubitemid 38938107)
11. Alvarez-Martinez MJ, Miro JM, Valls ME, et al. Sensitivity and specificity of nested and real-time PCR for the detection of Pneumocystis jiroveci in clinical specimens. Diagn Microbiol Infect Dis 2006; 56: 153-160. (Pubitemid 44488724)
12. Kovacs JA, Ng VL, Masur H, et al. Diagnosis of Pneumocystis carinii pneumonia: improved detection in sputum with use of monoclonal antibodies. N Engl J Med 1988; 318: 589-593. (Pubitemid 18083589)
13. Jacobs JA, Dieleman MM, Cornelissen EI, et al. Bronchoalveolar lavage fluid cytology in patients with Pneumocystis carinii pneumonia. Acta Cytol 2001; 45: 317-326. (Pubitemid 32472784)
14. Limper AH, Offord KP, Smith TF, et al. Pneumocystis carinii pneumonia. Differences in lung parasite number and inflammation in patients with and without AIDS. Am Rev Respir Dis 1989; 140: 1204-1209. (Pubitemid 19276197)
15. Thomas CF Jr, Limper AH. Current insights into the biology and pathogenesis of Pneumocystis pneumonia. Nat Rev Microbiol 2007; 5: 298-308. (Pubitemid 46443606)
16. Huggett JF, Taylor MS, Kocjan G, et al. Development and evaluation of a real-time PCR assay for detection of Pneumocystis jirovecii DNA in bronchoalveolar lavage fluid of HIV-infected patients. Thorax 2008; 63: 154-159. (Pubitemid 351236483)
17. Bandt D, Monecke S. Development and evaluation of a real-time PCR assay for detection of Pneumocystis jiroveci. Transpl Infect Dis 2007; 9: 196-202. (Pubitemid 47231213)
18. Arcenas RC, Uhl JR, Buckwalter SP, et al. A real-time polymerase chain reaction assay for detection of Pneumocystis from bronchoalveolar lavage fluid. Diagn Microbiol Infect Dis 2006; 54: 169-175.
19. Linssen CF, Jacobs JA, Beckers P, et al. Inter-laboratory comparison of three different real-time PCR assays for the detection of Pneumocystis jiroveci in bronchoalveolar lavage fluid samples. J Med Microbiol 2006; 55: 1229-1235. (Pubitemid 44373159)
20. Krajicek BJ, Limper AH, Thomas CF Jr. Advances in the biology, pathogenesis and identification of Pneumocystis pneumonia. Curr Opin Pulm Med 2008; 14: 228-234. (Pubitemid 351572324)
21. Nevez G, Raccurt C, Vincent P, et al. Pulmonary colonization with Pneumocystis carinii in human immunodeficiency virus-negative patients: assessing risk with blood CD4+ T cell counts. Clin Infect Dis 1999; 29: 1331-1332. (Pubitemid 30265664)
22. Maskell NA, Waine DJ, Lindley A, et al. Asymptomatic carriage of Pneumocystis jiroveci in subjects undergoing bronchoscopy: a prospective study. Thorax 2003; 58: 594-597. (Pubitemid 36808130)
23. Vargas SL, Ponce CA, Sanchez CA, et al. Pregnancy and asymptomatic carriage of Pneumocystis jiroveci. Emerg Infect Dis 2003; 9: 605-606. (Pubitemid 36529524)
24. Helweg-Larsen J, Jensen JS, Dohn B, et al. Detection of Pneumocystis DNA in samples from patients suspected of bacterial pneumonia - a case-control study. BMC Infect Dis 2002; 2: 28.
25. Flori P, Bellete B, Durand F, et al. Comparison between real-time PCR, conventional PCR and different staining techniques for diagnosing Pneumocystis jiroveci pneumonia from bronchoalveolar lavage specimens. J Med Microbiol 2004; 53: 603-607. (Pubitemid 38932356)
26. Larsen HH, Kovacs JA, Stock F, et al. Development of a rapid real-time PCR assay for quantitation of Pneumocystis carinii f. sp. carinii. J Clin Microbiol 2002; 40: 2989-2993. (Pubitemid 35284973)
27. Alanio A, Desoubeaux G, Sarfati C, et al. Real-time PCR assay-based strategy for differentiation between active Pneumocystis jirovecii pneumonia and colonization in immunocompromised patients. Clin Microbiol Infect 2011; 17: 1531-1537.
28. Azoulay E, Bergeron A, Chevret S, et al. Polymerase chain reaction for diagnosing Pneumocystis pneumonia in non-HIV immunocompromised patients with pulmonary infiltrates. Chest 2009; 135: 655-661.
29. Morris A, Wei K, Afshar K, et al. Epidemiology and clinical significance of pneumocystis colonization. J Infect Dis 2008; 197: 10-17. (Pubitemid 351263460)
30. Larsen HH, Masur H, Kovacs JA, et al. Development and evaluation of a quantitative, touch-down, real-time PCR assay for diagnosing Pneumocystis carinii pneumonia. J Clin Microbiol 2002; 40: 490-494. (Pubitemid 34130872)
31. Calderon EJ. Pneumocystis infection: seeing beyond the tip of the iceberg. Clin Infect Dis 2010; 50: 354-356.