Comparison between real-time PCR, conventional PCR and different staining techniques for diagnosing Pneumocystis jiroveci pneumonia from bronchoalveolar lavage specimens

Journal of Medical Microbiology

Volumn 53, Issue 7, 2004, Pages 603-607

  Flori, Pierre ^a   Bellete, Bahrie ^a   Durand, Fabrice ^a   Raberin, Hélène ^a   Cazorla, Céline ^b   Hafid, Jamal ^a   Lucht, Frédéric ^b   Sung, Roger Tran Mann ^a  

^a HÔPITAL NORD   (France)

^b SERVICE DE NEUROLOGIE   (France)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA;

ARTICLE; BACTERIAL GENE; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; DIAGNOSTIC TEST; GENE FREQUENCY; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS INFECTION; INTERMETHOD COMPARISON; LUNG LAVAGE; MAJOR CLINICAL STUDY; MICROBIOLOGICAL EXAMINATION; NONHUMAN; PNEUMOCYSTIS CARINII; PNEUMOCYSTIS PNEUMONIA; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; STAINING;

AIDS-RELATED OPPORTUNISTIC INFECTIONS; BRONCHOALVEOLAR LAVAGE FLUID; CARRIER STATE; DNA, FUNGAL; FALSE NEGATIVE REACTIONS; FALSE POSITIVE REACTIONS; HUMANS; IMMUNOCOMPROMISED HOST; PNEUMOCYSTIS JIROVECI; PNEUMONIA, PNEUMOCYSTIS; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; STAINING AND LABELING;

EID: 3142701273     PISSN: 00222615     EISSN: None     Source Type: Journal    
DOI: 10.1099/jmm.0.45528-0     Document Type: Article

References (20)

1. Caliendo, A. M., Hewitl, P. L., Allega, J. M., Keen, A., Ruoff, K. L. & Ferraro, M. J. (1998). Performance of a PCR assay for detection of Pneumocystis carinii from respiratory specimens. J Clin Microbiol 36, 979-982.
2. Cartwright, C. P., Nelson, N. A. & Gill, V. J. (1994). Development and evaluation of a rapid and simple procedure for detection of Pneumocystis carinii by PCR. J Clin Microbiol 32, 1634-1638.
3. Costa, J. M., Ernault, P., Gautier, E. & Bretagne, S. (2001). Prenatal diagnosis of congenital toxoplasmosis by duplex real-time PCR using fluorescence resonance energy transfer hybridization probes. Prenat Diagn 21, 85-88.
4. Cregan, P., Yamamoto, A., Lum, A., VanDerHeide, T., MacDonald, M. & Pulliam, L. (1990). Comparison of four methods for rapid detection of Pneumocystis carinii in respiratory specimens. J Clin Microbiol 28, 2432-2436.
5. Helweg-Larsen, J., Jensen, J. S., Dohn, B., Benfield, T. L. & Lundgren, B. (2002). Detection of Pneumocystis DNA in samples from patients suspected of bacterial pneumonia - a case-control study. BMC Infect Dis 2, 28. http://www.biomedcentral.com/1471-2334/2/28
6. Larsen, H. H., Masur, H., Kovacs, J. A. & 7 other authors (2002). Development and evaluation of a quantitative, touch-down, real-time PCR assay for diagnosing Pneumocystis carinii pneumonia. J Clin Microbiol 40, 490-494.
7. Leigh, T. R., Gazzard, B. G., Rowbottom, A. & Collins, J. V. (1993). Quantitative and qualitative comparison of DNA amplification by PCR with immunofluorescence staining for diagnosis of Pneumocystis carinii pneumonia. J Clin Pathol 46, 140-144.
8. Lipschik, G. Y., Gill, V. J., Lundgren, J. D., Andrawis, V. A., Nelson, N. A, Nielsen, J. O., Ognibene, F. P. & Kovacs, J. A. (1992). Improved diagnosis of Pneumocystis carinii infection by polymerase chain reaction on induced sputum and blood. Lancet 340, 203-206.
9. Maskell, N. A., Waine, D. J., Lindley, A., Pepperell, J. C., Wakefield, A. E., Miller, R. F. & Davies, R. J. (2003). Asymptomatic carriage of Pneumocystis jiroveci in subjects undergoing bronchoscopy: a prospective study. Thorax 58, 594-597.
10. Nevez, G., Raccurt, C., Jounieaux, V., Dei-Cas, E. & Mazars, E. (1999). Pneumocystosis versus pulmonary Pneumocystis carinii colonization in HIV-negative and HIV-positive patients. AIDS 13, 535-536.
11. Ninin, E., Hamidou, M., Germaud, P., Morin, O., Milpied, N. & Raffi, F. (1998). Pneumocystose pulmonaire chez les patients non VIH - étude rétrospective de 31 cas. Presse Méd 27, 244-249 (in French).
12. Ribes, J. A., Limper, A. H., Espy, M. J. & Smith, T. F. (1997). PCR detection of Pneumocystis carinii in bronchoalveolar lavage specimens: analysis of sensitivity and specificity. J Clin Microbiol 35, 830-835.
13. Roux, P., Lavrard, I., Poirot, J. L., Chouaid, C., Denis, M., Olivier, J. L., Nigou, M. & Miltgen, M. (1994). Usefulness of PCR for detection of Pneumocystis carinii DNA. J Clin Microbiol 32, 2324-2326.
14. Sing, A., Roggenkamp, A., Autenrieth, I. B. & Heesemann, J. (1999). Pneumocystis carinii carriage in immunocompetent patients with primary pulmonary disorders as detected by single or nested PCR. J Clin Microbiol 37, 3409-3410.
15. Sing, A., Trebesius, K., Roggenkamp, A., Russmann, H., Tybus, K., Pfaff, F., Bogner, J. R., Emminger, C. & Heesemann, J. (2000). Evaluation of diagnostic value and epidemiological implications of PCR for Pneumocystis carinii in different immunosuppressed and immunocompetent patient groups. J Clin Microbiol 38, 1461-1467.
16. Takahashi, T., Goto, M., Endo, T., Nakamura, T., Yusa, N., Sato, N. & Iwamoto, A. (2002). Pneumocystis carinii carriage in immunocompromised patients with and without human immunodeficiency virus infection. J Med Microbiol 51, 611-614.
17. Wakefield, A. E., Pixley, F. J., Banerji, S., Sinclair, K., Millier, R. F., Moxon, E. R. & Hopkin, J. M. (1990). Detection of Pneumocystis carinii with DNA amplification. Lancet 336, 451-453.
18. Wakefield, A. E., Guiver, L., Miller, R. F. & Hopkin, J. M. (1991). DNA amplification on induced sputum samples for diagnosis of Pneumocystis carinii pneumonia. Lancet 337, 1378-1379.
19. Weig, M., Klinker, H., Bogner, B. H., Meier, A. & Gross, U. (1997). Usefulness of PCR for diagnosis of Pneumocystis carinii pneumonia in different patient groups. J Clin Microbiol 35, 1445-1449.
20. Zaman, M. K., Wooten, O. J., Suprahmanya, B., Ankobiah, W., Finch, P. J. & Kamholz, S. L. (1988). Rapid noninvasive diagnosis of Pneumocystis carinii from induced liquefied sputum. Ann Intern Med 109, 7-10.