Real-time PCR assay for the diagnosis of pneumocystis jirovecii pneumonia

Methods in Molecular Biology

Volumn 943, Issue , 2013, Pages 159-170

  Fillaux, Judith ^a   Berry, Antoine ^a  

^a CHU RANGUEIL   (France)

Author keywords

Bronchoalveolar lavage; Diagnosis; Pneumocystis jirovecii; Real time PCR

Indexed keywords

BACTERIAL DNA; HEMOGLOBIN BETA CHAIN;

ARTICLE; GENETICS; HUMAN; ISOLATION AND PURIFICATION; LABORATORY DIAGNOSIS; METHODOLOGY; PNEUMOCYSTIS JIROVECI; PNEUMOCYSTIS PNEUMONIA; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

BETA-GLOBINS; DNA, BACTERIAL; HUMANS; PNEUMOCYSTIS JIROVECII; PNEUMONIA, PNEUMOCYSTIS; REAL-TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SPECIMEN HANDLING;

PNEUMOCYSTIS JIROVECII;

EID: 84870555679     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-60327-353-4_11     Document Type: Article

References (20)

1. Cisneros JM, Munoz P, Torre-Cisneros J, Gurgui M, Rodriguez-Hernandez MJ, Aguado JM et al (1998) Pneumonia after heart transplantation: a multi-institutional study. Spanish Transplantation Infection Study Group. Clin Infect Dis 27:324-331
2. Huang L, Morris A, Limper AH, Beck JM (2006) An Official ATS Workshop Summary: Recent advances and future directions in Pneumocystis pneumonia (PCP). Proc Am Thorac Soc 3:655-664
3. Sepkowitz KA (2002) Opportunistic infections in patients with and patients without Acquired Immunode ficiency Syndrome. Clin Infect Dis 34:1098-1107
4. Wakefield AE (2002) Pneumocystis carinii. Br Med Bull 61:175-188
5. DeLorenzo LJ, Huang CT, Maguire GP, Stone DJ (1987) Roentgenographic patterns of Pneumocystis carinii pneumonia in 104 patients with AIDS. Chest 91:323-327
6. Huang L, Stansell J, Osmond D, Turner J, Shafer KP, Fulkerson W et al (1999) Performance of an algorithm to detect Pneumocystis carinii pneumonia in symptomatic HIV-infected persons. Pulmonary Complications of HIV Infection Study Group. Chest 115:1025-1032
7. Dei-Cas E (2000) Pneumocystis infections: the iceberg? Med Mycol 38(Suppl 1):23-32
8. Thomas CF, Limper AH (2004) Pneumocystis pneumonia. N Engl J Med 350:2487-2498
9. Broaddus C, Dake MD, Stulbarg MS, Blumenfeld W, Hadley WK, Golden JA et al (1985) Bronchoalveolar lavage and transbronchial biopsy for the diagnosis of pulmonary infections in the acquired immunode ficiency syndrome. Ann Intern Med 102:747-752
10. Huang L, Hecht FM, Stansell JD, Montanti R, Hadley WK, Hopewell PC (1995) Suspected Pneumocystis carinii pneumonia with a negative induced sputum examination. Is early bronchoscopy useful? Am J Respir Crit Care Med 151:1866-1871
11. Calderon EJ, Regordan C, Medrano FJ, Ollero M, Varela JM (1996) Pneumocystis carinii infection in patients with chronic bronchial disease. Lancet 347:977
12. Helweg-Larsen J, Jensen JS, Dohn B, Ben field TL, Lundgren B (2002) Detection of Pneumocystis DNA in samples from patients suspected of bacterial pneumonia-a case-control study. BMC Infect Dis 2:28
13. Maskell NA, Waine DJ, Lindley A, Pepperell JC, Wakefield AE, Miller RF et al (2003) Asymptomatic carriage of Pneumocystis jiroveci in subjects undergoing bronchoscopy: a prospective study. Thorax 58:594-597
14. Miller RF, Ambrose HE, Wakefield AE (2001) Pneumocystis carinii f. sp. hominis DNA in immunocompetent health care workers in contact with patients with P. carinii pneumonia. J Clin Microbiol 39:3877-3882
15. Nevez G, Raccurt C, Jounieaux V, Dei-Cas E, Mazars E (1999) Pneumocystosis versus pulmonary Pneumocystis carinii colonization in HIV-negative and HIV-positive patients. AIDS 13:535-536
16. Fillaux J, Malvy S, Alvarez M, Fabre R, Cassaing S, Marchou B et al (2008) Accuracy of a routine real-time PCR assay for the diagnosis of Pneumocystis jirovecii pneumonia. J Microbiol Methods 75:258-261
17. Larsen HH, Masur H, Kovacs JA, Gill VJ, Silcott VA, Kogulan P et al (2002) Development and evaluation of a quantitative, touch-down, realtime PCR assay for diagnosing Pneumocystis carinii pneumonia. J Clin Microbiol 40:490-494
18. Saiki RK, Scharf S, Faloona F, Mullis KB, Horn GT, Erlich HA et al (1985) (1992) Enzymatic ampli fication of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Biotechnology 24:476-480
19. Rennard SI, Ghafouri M, Thompson AB, Linder J, Vaughan W, Jones K et al (1990) Fractional processing of sequential bronchoalveolar lavage to separate bronchial and alveolar samples. Am Rev Respir Dis 141:208-217
20. Bustin SA (2000) Absolute quanti fication of mRNA using real-time reverse transcription polymerase chain reaction assays. J Mol Endocrinol 25:169-193