Increased enantioselectivity by engineering bottleneck mutants in an esterase from Pseudomonas fluorescens

ChemBioChem

Volumn 10, Issue 18, 2009, Pages 2920-2923

  Schließmann, Anna ^a   Hidalgo, Aurelio ^b   Berenguer, José ^b   Bornscheuer, Uwe T ^a  

^a UNIVERSITY OF GREIFSWALD   (Germany)

^b CENTRO DE BIOLOGÍA MOLECULAR SEVERO OCHOA   (Spain)

Author keywords

Enantioselectivity; Enzyme catalysis; Esterases; Focused directed evolution; Protein engineering

Indexed keywords

1 PHENYL 1 PROPYL ACETATE; ACETIC ACID; ESTERASE; PSEUDOMONAS FLUORESCENS ESTERASE 1; UNCLASSIFIED DRUG;

ARTICLE; CALCULATION; ENANTIOSELECTIVITY; ENZYME ACTIVE SITE; ENZYME SPECIFICITY; ENZYME STRUCTURE; ENZYME SUBSTRATE; HYDROPHOBICITY; MOLECULAR DOCKING; MUTATION; NONHUMAN; PRIORITY JOURNAL; PSEUDOMONAS FLUORESCENS;

AMINO ACID SUBSTITUTION; BIOCATALYSIS; CATALYTIC DOMAIN; COMPUTER SIMULATION; ESTERASES; HYDROPHOBICITY; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; PROTEIN ENGINEERING; PSEUDOMONAS FLUORESCENS; RECOMBINANT PROTEINS; STEREOISOMERISM; SUBSTRATE SPECIFICITY;

PSEUDOMONAS FLUORESCENS;

EID: 72949108711     PISSN: 14394227     EISSN: 14397633     Source Type: Journal    
DOI: 10.1002/cbic.200900563     Document Type: Article

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