Impact of normalization strategy on cardiac expression of pro-inflammatory cytokines: Evaluation of reference genes in different human myocardial regions after Left Ventricular Assist Device support

Cytokine

Volumn 63, Issue 2, 2013, Pages 113-122

  Caselli, Chiara ^a   D'Amico, Andrea ^b   Caruso, Raffaele ^c   Cabiati, Manuela ^a   Prescimone, Tommaso ^a   Cozzi, Lorena ^c   Cannata, Aldo ^c   Parodi, Oberdan ^c   Del Ry, Silvia ^a   Giannessi, Daniela ^a  

^a INSTITUTE OF CLINICAL PHYSIOLOGY   (Italy)

^b SCUOLA SUPERIORE SANT ANNA   (Italy)

^c OSPEDALE NIGUARDA CA GRANDA   (Italy)

Author keywords

Cardiac cytokines; Gene reference; Heart transplantation; LVAD; Normalization strategy

Indexed keywords

ANGIOTENSIN RECEPTOR ANTAGONIST; BETA ADRENERGIC RECEPTOR BLOCKING AGENT; DIPEPTIDYL CARBOXYPEPTIDASE INHIBITOR; HYDROXYMETHYLGLUTARYL COENZYME A REDUCTASE INHIBITOR; INOTROPIC AGENT; INTERLEUKIN 6; INTERLEUKIN 8; MESSENGER RNA; TUMOR NECROSIS FACTOR ALPHA;

ACTB GENE; ADULT; ARTICLE; CARDIAC REMODELING; CLINICAL ARTICLE; CONTROLLED STUDY; EEF1A GENE; FEMALE; GAPDH GENE; GENE; GENE EXPRESSION; GENETIC ANALYSIS; GRAFT RECIPIENT; HEART FAILURE; HEART TRANSPLANTATION; HPRT1 GENE; HUMAN; HUMAN TISSUE; IL 6 GENE; IL 8 GENE; INFLAMMATION; LEFT VENTRICULAR ASSIST DEVICE; MALE; NUCLEOTIDE SEQUENCE; PPIA GENE; PRIORITY JOURNAL; PROTEIN EXPRESSION; RELIABILITY; RPL13A GENE; TISSUE REACTION; TOP2B GENE; TPT1 GENE; TUMOR NECROSIS FACTOR ALPHA GENE; VALIDATION STUDY; YWHAZ GENE;

14-3-3 PROTEINS; ADULT; CYCLOPHILIN A; FEMALE; HEART; HEART FAILURE; HEART TRANSPLANTATION; HEART-ASSIST DEVICES; HUMANS; INTERLEUKIN-6; INTERLEUKIN-8; MALE; MIDDLE AGED; RIBOSOMAL PROTEINS; RNA, MESSENGER; TUMOR NECROSIS FACTOR-ALPHA; VENTRICULAR REMODELING;

EID: 84878997640     PISSN: 10434666     EISSN: 10960023     Source Type: Journal    
DOI: 10.1016/j.cyto.2013.04.021     Document Type: Article

References (31)

1. Dickstein K., Cohen-Solal A., Filippatos G., McMurray J.J., Ponikowski P., et al. ESC guidelines for the diagnosis and treatment of acute and chronic heart failure 2008: The task force for the diagnosis and treatment of acute and chronic heart failure 2008 of the European Society of Cardiology. Developed in collaboration with the Heart Failure Association of the ESC (HFA) and endorsed by the European Society of Intensive Care Medicine (ESICM). Eur J Heart Fail 2008, 10(10):933-989.
2. Roger V.L., Go A.S., Lloyd-Jones D.M., Adams R.J., Berry J.D., et al. American Heart Association Statistics Committee and Stroke Statistics Subcommittee. Heart disease and stroke statistics-2011 update: a report from the American Heart Association. Circulation 2011, 123(4):e18-e209.
3. Lloyd-Jones D., Adams R.J., Brown T.M., Carnethon M., Dai S., et al. Heart disease and stroke statistics-2010 update: a report from the American Heart Association. Circulation 2010, 121(7):e46-e215.
4. Levy D., Kenchaiah S., Larson M.G., Benjamin E.J., Kupka M.J., et al. Long-term trends in the incidence of and survival with heart failure. New Engl J Med 2002, 347(18):1397-1402.
5. Delgado R.M., Radovancevic B. Symptomatic relief: left ventricular assist devices versus resynchronization therapy. Heart Fail Clin 2007, 3(3):259-265.
6. Wilson S.R., Givertz M.M., Stewart G.C., Mudge G.H. Ventricular assist devices the challenges of outpatient management. J Am Coll Cardiol 2009, 54(18):1647-1659.
7. Birks E.J., George R.S. Molecular changes occurring during reverse remodelling following left ventricular assist device support. J Cardiovasc Transl Res 2010, 3(6):635-642.
8. Hall J.L., Fermin D.R., Birks E.J., Barton P.J., Slaughter M., et al. Clinical, molecular, and genomic changes in response to a left ventricular assist device. J Am Coll Cardiol 2011, 57(6):641-652.
9. Birks E.J., Latif N., Owen V., Bowles C., Felkin L.E., et al. Quantitative myocardial cytokine expression and activation of the apoptotic pathway in patients who require left ventricular assist devices. Circulation 2001, 104(12 Suppl):I233-I240.
10. Bedi M.S., Alvarez R.J., Kubota T., Sheppard R., Kormos R.L., et al. Myocardial fas and cytokine expression in end-stage heart failure: impact of LVAD support. Clin Transl Sci 2008, 1(3):245-248.
11. Bustin S.A. Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems. J Mol Endocrinol 2002, 29(1):23-39.
12. Nolan T., Hands R.E., Bustin S.A. Quantification of mRNA using real-time RT-PCR. Nat Protoc 2006, 1(3):1559-1582.
13. Bustin S.A. Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays. J Mol Endocrinol 2000, 25(2):169-193.
14. Derveaux S., Vandesompele J., Hellemans J. How to do successful gene expression analysis using real-time PCR. Methods 2010, 50(4):227-230.
15. Vandesompele J., De Preter K., Pattyn F., Poppe B., Van Roy N., et al. Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol 2002, 3(7). RESEARCH0034.
16. Martino A., Cabiati M., Campan M., Prescimone T., Minocci D., et al. Selection of reference genes for normalization of real-time PCR data in minipig heart failure model and evaluation of TNF-α mRNA expression. J Biotechnol 2011, 153(3-4):92-99.
17. Cabiati M., Raucci S., Caselli C., Guzzardi M.A., D'Amico A., et al. Tissue-specific selection of stable reference genes for real-time PCR normalization in an obese rat model. J Mol Endocrinol 2012, 48(3):251-260.
18. Brattelid T., Tveit K., Birkeland J.A., Sjaastad I., Qvigstad E., et al. Expression of mRNA encoding G protein-coupled receptors involved in congestive heart failure-a quantitative RT-PCR study and the question of normalisation. Basic Res Cardiol 2007, 102(3):198-208.
19. Brattelid T., Winer L.H., Levy F.O., Liestøl K., Sejersted O.M., et al. Reference gene alternatives to GAPDH in rodent and human heart failure gene expression studies. BMC Mol Biol 2010, 11(Mar 23):22.
20. Everaert B.R., Boulet G.A., Timmermans J.P., Vrints C.J. Importance of suitable reference gene selection for quantitative real-time PCR: special reference to mouse myocardial infarction studies. PLoS One 2011, 6(8):e23793.
21. Vesentini N., Barsanti C., Martino A., Kusmic C., Ripoli A., Rossi A., et al. Selection of reference genes in different myocardial regions of an in vivo ischemia/reperfusion rat model for normalization of antioxidant gene expression. BMC Res Notes 2012, 5(Feb 29):124.
22. Pilbrow A.P., Ellmers L.J., Black M.A., Moravec C.S., Sweet W.E., et al. Genomic selection of reference genes for real-time PCR in human myocardium. BMC Med Genomics 2008.
23. Bustin S.A., Benes V., Garson J.A., Hellemans J., Huggett J., et al. The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin Chem 2009, 55(4):611-622.
24. Andersen C.L., Jensen J.L., Ørntoft T.F. Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization applied to bladder and colon cancer data sets. Cancer Res 2004, 64(1):5245-5250.
25. Mehta R., Birerdinc A., Hossain N., Afendy A., Chandhoke V., Younossi Z., et al. Validation of endogenous reference genes for qRT-PCR analysis of human visceral adipose samples. BMC Mol Biol 2010, 11:39.
26. Sørby L.A., Andersen S.N., Bukholm I.R., Jacobsen M.B. Evaluation of suitable reference genes for normalization of real-time reverse transcription PCR analysis in colon cancer. J Exp Clin Cancer Res 2010, 29:144.
27. Heymans S., Hirsch E., Anker S.D., Aukrust P., Balligand J.L., Cohen-Tervaert J.W., et al. Inflammation as therapeutic target in HF? A scientific statement from the translational research committee of the HF Association of the European Society of Cardiology. Eur J Heart Fail 2009, 11:119-129.
28. Kubota T., Masayuki M., Alvarez R., Kormos R., Rosenblum W., et al. Expression of proinflammatory cytokines in the failing human heart: comparison of recent onset and end stage heart failure. J Heart Lung Trans 2000, 19(9):819-824.
29. Blum A., Miller H. Role of cytokines in heart failure. Am Heart J 1998, 135:181-186.
30. Mann D.M. Inflammatory mediators and failing heart. Past, present and the foreseeable future. Circ Res 2002, 91:988-998.
31. Caruso R., Caselli C., Boroni C., Campolo J., Milazzo F., et al. Relationship between myocardial redox state and matrix metalloproteinase activity in patients on left ventricular assist device support. Circ J 2011, 75(10):2387-2396.