Comparison of quantitative PCR and flow cytometry as cellular viability methods to study bacterial membrane permeabilization following supercritical CO2 treatment

Microbiology (United Kingdom)

Volumn 159, Issue PART 6, 2013, Pages 1056-1066

  Tamburini, Sabrina ^a   Ballarini, Annalisa ^a   Ferrentino, Giovanna ^a   Moro, Albertomaria ^a   Foladori, Paola ^a   Spilimbergo, Sara ^a   Jousson, Olivier ^a  

^a UNIVERSITY OF TRENTO   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

AZIDE; CARBON DIOXIDE; PROPIDIUM IODIDE; PROPIDIUM MONOAZIDE; UNCLASSIFIED DRUG;

ANALYTIC METHOD; ARTICLE; BACTERIAL MEMBRANE; CELL COUNT; CELL DIVISION; CELL SURFACE; CELL VIABILITY; ENVIRONMENTAL FACTOR; ESCHERICHIA COLI; FLOW CYTOMETRY; FLUORESCENCE MICROSCOPY; FOOD CHAIN; IN VITRO STUDY; LISTERIA MONOCYTOGENES; MEMBRANE PERMEABILITY; MICROBIOLOGICAL EXAMINATION; NONHUMAN; PASTEURIZATION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RISK ASSESSMENT; SALMONELLA ENTERICA;

BACTERIAL LOAD; CARBON DIOXIDE; CELL MEMBRANE; CELL MEMBRANE PERMEABILITY; DISINFECTANTS; FLOW CYTOMETRY; FOOD MICROBIOLOGY; GRAM-NEGATIVE BACTERIA; MICROBIAL VIABILITY; REAL-TIME POLYMERASE CHAIN REACTION;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; LISTERIA MONOCYTOGENES; SALMONELLA ENTERICA;

EID: 84878837167     PISSN: 13500872     EISSN: 14652080     Source Type: Journal    
DOI: 10.1099/mic.0.063321-0     Document Type: Article

References (31)

1. Anon (2002). Risk profile on the microbiological contamination of fruits and vegetables eaten raw: Report of the Scientific Committee on Food. Brussels: SCF/CS/FMH/SURF.
2. Bae, Y. Y., Choi, Y. M., Kim, M. J., Kim, K. H., Kim, B. C. & Rhee, M. S. (2011). Application of supercritical carbon dioxide for microorganism reduction in fresh pork. J Food Saf 31, 511-517.
3. Ballestra, P., Abreu da Silva, A. & Cuq, J. L. (1996). Inactivation of Escherichia coli by carbon dioxide under pressure. J Food Sci 61, 829-831.
4. Barbesti, S., Citterio, S., Labra, M., Baroni, M. D., Neri, M. G. & Sgorbati, S. (2000). Two and three-color fluorescence flow cytometric analysis of immunoidentified viable bacteria. Cytometry 40, 214-218.
5. Berney, M., Hammes, F., Bosshard, F., Weilenmann, H. U. & Egli, T. (2007). Assessment and interpretation of bacterial viability by using the LIVE/DEAD BacLight Kit in combination with flow cytometry. Appl Environ Microbiol 73, 3283-3290.
6. Devlieghere, F., Vermeiren, L. & Debevere, J. (2004). New preservation technologies: possibilities and limitations. Int Dairy J 14, 273-285.
7. Erkmen, O. (2000). Effect of carbon dioxide pressure on Listeria monocytogenes in physiological saline and foods. Food Microbiol 17, 589-596.
8. Erkmen, O. & Karaman, H. (2001). Kinetic studies on the high pressure carbon dioxide inactivation of Salmonella typhimurium. J Food Eng 50, 25-28.
9. Ferrentino, G. & Spilimbergo, S. (2011). High pressure carbon dioxide pasteurization of solid foods: current knowledge and future outlooks. Trends Food Sci Technol 22, 427-441.
10. Foladori, P., Quaranta, A. & Ziglio, G. (2008). Use of silica microspheres having refractive index similar to bacteria for conversion of flow cytometric forward light scatter into biovolume. Water Res 42, 3757-3766.
11. Foladori, P., Bruni, L., Tamburini, S. & Ziglio, G. (2010). Direct quantification of bacterial biomass in influent, effluent and activated sludge of wastewater treatment plants by using flow cytometry. Water Res 44, 3807-3818.
12. Gandhi, M. & Chikindas, M. L. (2007). Listeria: a foodborne pathogen that knows how to survive. Int J Food Microbiol 113, 1-15.
13. Garcia-Gonzalez, L., Geeraerd, A. H., Spilimbergo, S., Elst, K., Van Ginneken, L., Debevere, J., Van Impe, J. F. & Devlieghere, F. (2007). High pressure carbon dioxide inactivation of microorganisms in foods: the past, the present and the future. Int J Food Microbiol 117, 1-28.
14. Garcia-Gonzalez, L., Geeraerd, A. H., Mast, J., Briers, Y., Elst, K., Van Ginneken, L., Van Impe, J. F. & Devlieghere, F. (2010). Membrane permeabilization and cellular death of Escherichia coli, Listeria monocytogenes and Saccharomyces cerevisiae as induced by high pressure carbon dioxide treatment. Food Microbiol 27, 541-549.
15. Jung, W. Y., Choi, Y. M. & Rhee, M. S. (2009). Potential use of supercritical carbon dioxide to decontaminate Escherichia coli O157: H7, Listeria monocytogenes, and Salmonella typhimurium in alfalfa sprouted seeds. Int J Food Microbiol 136, 66-70.
16. Keer, J. T. & Birch, L. (2003). Molecular methods for the assessment of bacterial viability. J Microbiol Methods 53, 175-183.
17. Kim, H. T., Choi, H. J. & Kim, K. H. (2009). Flow cytometric analysis of Salmonella enterica serotype Typhimurium inactivated with supercritical carbon dioxide. J Microbiol Methods 78, 155-160.
18. Klein, D. (2002). Quantification using real-time PCR technology: applications and limitations. Trends Mol Med 8, 257-260.
19. Liao, H., Zhang, F., Liao, X., Hu, X., Chen, Y. & Deng, L. (2010). Analysis of Escherichia coli cell damage induced by HPCD using microscopies and fluorescent staining. Int J Food Microbiol 144, 169-176.
20. Mantoan, D. & Spilimbergo, S. (2011). Mathematical modeling of yeast inactivation of freshly squeezed apple juice under high-pressure carbon dioxide. Crit Rev Food Sci Nutr 51, 91-97.
21. Müller, S. & Nebe-von-Caron, G. (2010). Functional single-cell analyses: flow cytometry and cell sorting of microbial populations and communities. FEMS Microbiol Rev 34, 554-587.
22. Nocker, A. & Camper, A. K. (2006). Selective removal of DNA from dead cells of mixed bacterial communities by use of ethidium monoazide. Appl Environ Microbiol 72, 1997-2004.
23. Nocker, A., Sossa-Fernandez, P., Burr, M. D. & Camper, A. K. (2007a). Use of propidium monoazide for live/dead distinction in microbial ecology. Appl Environ Microbiol 73, 5111-5117.
24. Nocker, A., Sossa, K. E. & Camper, A. K. (2007b). Molecular monitoring of disinfection efficacy using propidium monoazide in combination with quantitative PCR. J Microbiol Methods 70, 252-260.
25. Oliver, J. D. (2010). Recent findings on the viable but nonculturable state in pathogenic bacteria. FEMS Microbiol Rev 34, 415-425.
26. Spilimbergo, S. & Bertucco, A. (2003). Non-thermal bacterial inactivation with dense CO2. Biotechnol Bioeng 84, 627-638.
27. Suo, B., He, Y., Tu, S. I. & Shi, X. (2010). A multiplex real-time polymerase chain reaction for simultaneous detection of Salmonella spp., Escherichia coli O157, and Listeria monocytogenes in meat products. Foodborne Pathog Dis 7, 619-628.
28. Velusamy, V., Arshak, K., Korostynska, O., Oliwa, K. & Adley, C. (2010). An overview of foodborne pathogen detection: in the perspective of biosensors. Biotechnol Adv 28, 232-254.
29. WHO (2007). Global Public Health Security in the 21st Century. Geneva: World Health Organization.
30. Wouters, P. C., Bos, A. P. & Ueckert, J. (2001). Membrane permeabilization in relation to inactivation kinetics of Lactobacillus species due to pulsed electric fields. Appl Environ Microbiol 67, 3092-3101.
31. Ziglio, G., Andreottola, G., Barbesti, S., Boschetti, G., Bruni, L., Foladori, P. & Villa, R. (2002). Assessment of activated sludge viability with flow cytometry. Water Res 36, 460-468.