Differential expression of human cytochrome P450 enzymes from the CYP3A subfamily in the brains of alcoholic subjects and drug-free controls

Drug Metabolism and Disposition

Volumn 41, Issue 6, 2013, Pages 1187-1194

  Depaz, Iris M Booth ^a   Toselli, Francesca ^a   Wilce, Peter A ^a   Gillam, Elizabeth M J ^a  

^a UNIVERSITY OF QUEENSLAND   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOCHROME P450 3A4; CYTOCHROME P450 3A5; CYTOCHROME P450 3A7;

ADULT; ALCOHOLISM; AMINO TERMINAL SEQUENCE; AMYGDALOID NUCLEUS; ANTIGEN DETECTION; ARTICLE; AXONAL HILLOCK; BASAL GANGLION; BRAIN MICROSOME; CELL COMPARTMENTALIZATION; CELL LAYER; CELL TYPE; CELLULAR DISTRIBUTION; CELLULAR PARAMETERS; CEREBELLUM; CLINICAL ARTICLE; CONTROLLED STUDY; CROSS REACTION; ENZYME ANALYSIS; ENZYME LOCALIZATION; FRONTAL CORTEX; HIPPOCAMPUS; HUMAN; HUMAN CELL; HUMAN TISSUE; IMMUNOREACTIVITY; MALE; MEDICAL HISTORY; NERVE FIBER; PRIORITY JOURNAL; PROTEIN EXPRESSION; PYRAMIDAL NERVE CELL; SOMATIC CELL;

ADULT; ALCOHOLICS; ALCOHOLISM; ANIMALS; BRAIN; CYTOCHROME P-450 CYP3A; CYTOCHROME P-450 ENZYME SYSTEM; GENE EXPRESSION REGULATION, ENZYMOLOGIC; HUMANS; MALE; MIDDLE AGED; RABBITS;

EID: 84878256172     PISSN: 00909556     EISSN: 1521009X     Source Type: Journal    
DOI: 10.1124/dmd.113.051359     Document Type: Article

References (50)

1. Agarwal V, Kommaddi RP, Valli K, Ryder D, Hyde TM, Kleinman JE, Strobel HW, and Ravindranath V (2008) Drug metabolism in human brain: high levels of cytochrome P4503A43 in brain and metabolism of anti-anxiety drug alprazolam to its active metabolite. PLoS ONE 3:e2337.
2. Avadhani NG, Sangar MC, Bansal S, and Bajpai P (2011) Bimodal targeting of cytochrome P450s to endoplasmic reticulum and mitochondria: the concept of chimeric signals. FEBS J 278:4218-4229.
3. Britto MR and Wedlund PJ (1992) Cytochrome P-450 in the brain. Potential evolutionary and therapeutic relevance of localization of drug-metabolizing enzymes. Drug Metab Dispos 20:446-450.
4. Daly AK (2006) Significance of the minor cytochrome P450 3A isoforms. Clin Pharmacokinet 45:13-31.
5. Dauchy S, Dutheil F, Weaver RJ, Chassoux F, Daumas-Duport C, Couraud PO, Scherrmann JM, De Waziers I, and Declèves X (2008) ABC transporters, cytochromes P450 and their main transcription factors: expression at the human blood-brain barrier. J Neurochem 107:1518-1528.
6. Depaz I, Ito M, Matsumoto I, Niwa S, Kroon P, and Wilce PA (2003) Expression of hNP22 is altered in the frontal cortex and hippocampus of the alcoholic human brain. Alcohol Clin Exp Res 27:1481-1488.
7. Dutheil F, Dauchy S, Diry M, Sazdovitch V, Cloarec O, Mellottée L, Bièche I, Ingelman-Sundberg M, Flinois JP, and De Waziers I, et al. (2009) Xenobiotic-metabolizing enzymes and transporters in the normal human brain: regional and cellular mapping as a basis for putative roles in cerebral function. Drug Metab Dispos 37:1528-1538.
8. Dutheil F, Jacob A, Dauchy S, Beaune P, Scherrmann JM, Declèves X, and Loriot MA (2010) ABC transporters and cytochromes P450 in the human central nervous system: influence on brain pharmacokinetics and contribution to neurodegenerative disorders. Expert Opin Drug Metab Toxicol 6:1161-1174.
9. Farin FM and Omiecinski CJ (1993) Regiospecific expression of cytochrome P-450s and microsomal epoxide hydrolase in human brain tissue. J Toxicol Environ Health 40:317-335.
10. Feierman DE, Melinkov Z, and Nanji AA (2003) Induction of CYP3A by ethanol in multiple in vitro and in vivo models. Alcohol Clin Exp Res 27:981-988.
11. Ghosh C, Gonzalez-Martinez J, Hossain M, Cucullo L, Fazio V, Janigro D, and Marchi N (2010) Pattern of P450 expression at the human blood-brain barrier: roles of epileptic condition and laminar flow. Epilepsia 51:1408-1417.
12. Ghosh C, Marchi N, Desai NK, Puvenna V, Hossain M, Gonzalez-Martinez J, Alexopoulos AV, and Janigro D (2011) Cellular localization and functional significance of CYP3A4 in the human epileptic brain. Epilepsia 52:562-571.
13. Gillam E M J, Baba T, Kim B-R, Ohmori S, and Guengerich FP (1993) Expression of modified human cytochrome P450 3A4 in Escherichia coli and purification and reconstitution of the enzyme. Arch Biochem Biophys 305:123-131.
14. Gillam E M J, Guo Z, Ueng Y-F, Yamazaki H, Cock I, Reilly P E B, Hooper WD, and Guengerich FP (1995) Expression of cytochrome P450 3A5 in Escherichia coli: effects of 59 modification, purification, spectral characterization, reconstitution conditions, and catalytic activities. Arch Biochem Biophys 317:374-384.
15. Guengerich FP (2005) Human cytochrome P450 enzymes, in Cytochrome P450: Structure, Mechanism, and Biochemistry, 3rd ed (Ortiz de Montellano PR ed) pp 377-530, Kluwer Academic/Plenum Publishing, New York.
16. Haining RL and Nichols-Haining M (2007) Cytochrome P450-catalyzed pathways in human brain: metabolism meets pharmacology or old drugs with new mechanism of action? Pharmacol Ther 113:537-545.
17. Harlow E and Lane D (1999) Using Antibodies: A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
18. Hoshino U and Kawasaki H (1995) Urinary 6 beta-hydroxycortisol excretion in patients with alcoholic liver disease. Research Communications in Alcohol and Substances of Abuse 16:115-124.
19. Jayyosi Z, Cooper KO, and Thomas PE (1992) Brain cytochrome P450 and testosterone metabolism by rat brain subcellular fractions: presence of cytochrome P450 3A immunoreactive protein in rat brain mitochondria. Arch Biochem Biophys 298:265-270.
20. Jeon S, Kim KH, Yun CH, Hong BW, Chang YS, Han HS, Yoon YS, Choi WB, Kim S, and Lee AY (2008) An NH2-terminal truncated cytochrome P450 CYP3A4 showing catalytic activity is present in the cytoplasm of human liver cells. Exp Mol Med 40:254-260.
21. Kirches E, Scherlach C, Von Bossanyi P, Schneider T, Szibor R, Kutz E, Warich-Kirches M, and Dietzmann K (1999) MGMT-and P450 3A-inhibitors do not sensitize glioblastoma cell cultures against nitrosoureas. Clin Neuropathol 18:1-8.
22. Koop DR and Tierney DJ (1990) Multiple mechanisms in the regulation of ethanol-inducible cytochrome P450IIE1. Bioessays 12:429-435.
23. Kostrubsky VE, Strom SC, Wood SG, Wrighton SA, Sinclair PR, and Sinclair JF (1995) Ethanol and isopentanol increase CYP3A and CYP2E in primary cultures of human hepatocytes. Arch Biochem Biophys 322:516-520.
24. Krishna DR and Shekar MS (2005) Cytochrome P450 3A: genetic polymorphisms and interethnic differences. Methods Find Exp Clin Pharmacol 27:559-567.
25. Lacroix D, Sonnier M, Moncion A, Cheron G, and Cresteil T (1997) Expression of CYP3A in the human liver-evidence that the shift between CYP3A7 and CYP3A4 occurs immediately after birth. Eur J Biochem 247:625-634.
26. Lamba V, Yasuda K, Lamba JK, Assem M, Davila J, Strom S, and Schuetz EG (2004) PXR (NR1I2): splice variants in human tissues, including brain, and identification of neurosteroids and nicotine as PXR activators. Toxicol Appl Pharmacol 199:251-265.
27. Liangpunsakul S, Kolwankar D, Pinto A, Gorski JC, Hall SD, and Chalasani N (2005) Activity of CYP2E1 and CYP3A enzymes in adults with moderate alcohol consumption: a comparison with nonalcoholics. Hepatology 41:1144-1150.
28. Mann A, Miksys S, Lee A, Mash DC, and Tyndale RF (2008) Induction of the drug metabolizing enzyme CYP2D in monkey brain by chronic nicotine treatment. Neuropharmacology 55:1147-1155.
29. McFadyen MC, Melvin WT, and Murray GI (1998) Regional distribution of individual forms of cytochrome P450 mRNA in normal adult human brain. Biochem Pharmacol 55:825-830.
30. Meyer RP and Gehlhaus M (2010) A role for CYP in the drug-hormone crosstalk of the brain. Expert Opin Drug Metab Toxicol 6:675-687.
31. Meyer RP, Gehlhaus M, Knoth R, and Volk B (2007) Expression and function of cytochrome p450 in brain drug metabolism. Curr Drug Metab 8:297-306.
32. Meyer RP, Gehlhaus M, Schwab R, Bürck C, Knoth R, and Hagemeyer CE (2009) Concordant up-regulation of cytochrome P450 Cyp3a11, testosterone oxidation and androgen receptor expression in mouse brain after xenobiotic treatment. J Neurochem 109:670-681.
33. Meyer RP, Podvinec M, and Meyer UA (2002) Cytochrome P450 CYP1A1 accumulates in the cytosol of kidney and brain and is activated by heme. Mol Pharmacol 62:1061-1067.
34. Murray GI, Pritchard S, Melvin WT, and Burke MD (1995) Cytochrome P450 CYP3A5 in the human anterior pituitary gland. FEBS Lett 364:79-82.
35. Neve EP and Ingelman-Sundberg M (2008) Intracellular transport and localization of microsomal cytochrome P450. Anal Bioanal Chem 392:1075-1084.
36. Nishimura M, Yaguti H, Yoshitsugu H, Naito S, and Satoh T (2003) Tissue distribution of mRNA expression of human cytochrome P450 isoforms assessed by high-sensitivity real-time reverse transcription PCR. Yakugaku Zasshi 123:369-375.
37. Niwa T, Murayama N, Emoto C, and Yamazaki H (2008) Comparison of kinetic parameters for drug oxidation rates and substrate inhibition potential mediated by cytochrome P450 3A4 and 3A5. Curr Drug Metab 9:20-33.
38. Niwa T, Murayama N, and Yamazaki H (2009) Oxidation of endobiotics mediated by xenobioticmetabolizing forms of human cytochrome. Curr Drug Metab 10:700-712.
39. Pai HV, Upadhya SC, Chinta SJ, Hegde SN, and Ravindranath V (2002) Differential metabolism of alprazolam by liver and brain cytochrome (P4503A) to pharmacologically active metabolite. Pharmacogenomics J 2:243-258.
40. Patki KC, Greenblatt DJ, and Von Moltke LL (2004) Ethanol inhibits in-vitro metabolism of nifedipine, triazolam and testosterone in human liver microsomes. J Pharm Pharmacol 56:963-966.
41. Song BJ, Veech RL, Park SS, Gelboin HV, and Gonzalez FJ (1988) Structure and regulation of the ethanol-inducible cytochrome P450j. Adv Alcohol Subst Abuse 7:205-207.
42. Stevens JC (2006) New perspectives on the impact of cytochrome P450 3A expression for pediatric pharmacology. Drug Discov Today 11:440-445.
43. Stevens JC, Hines RN, Gu C, Koukouritaki SB, Manro JR, Tandler PJ, and Zaya MJ (2003) Developmental expression of the major human hepatic CYP3A enzymes. J Pharmacol Exp Ther 307:573-582.
44. Strobel HW, Thompson CM, and Antonovic L (2001) Cytochromes P450 in brain: function and significance. Curr Drug Metab 2:199-214.
45. Tateishi T, Nakura H, Asoh M, Watanabe M, Tanaka M, Kumai T, Takashima S, Imaoka S, Funae Y, and Yabusaki Y, et al. (1997) A comparison of hepatic cytochrome P450 protein expression between infancy and postinfancy. Life Sci 61:2567-2574.
46. Towbin H, Staehelin T, and Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76:4350-4354.
47. Voirol P, Jonzier-Perey M, Porchet F, Reymond MJ, Janzer RC, Bouras C, Strobel HW, Kosel M, Eap CB, and Baumann P (2000) Cytochrome P-450 activities in human and rat brain microsomes. Brain Res 855:235-243.
48. Wienkers LC and Heath TG (2005) Predicting in vivo drug interactions from in vitro drug discovery data. Nat Rev Drug Discov 4:825-833.
49. Wójcikowski J, Golembiowska K, and Daniel WA (2007) The regulation of liver cytochrome p450 by the brain dopaminergic system. Curr Drug Metab 8:631-638.
50. Worrall S, De Jersey J, and Wilce PA (1993) Antibodies from alcoholics, ethanol-fed rats and a rabbit immunised with proteins modified by acetaldehyde in vitro react with liver cytosolic proteins from ethanol-fed rats. Alcohol Alcohol 28:513-519.