Direct imaging of ER calcium with targeted-esterase induced dye loading (TED)

Journal of Visualized Experiments

Volumn , Issue 75, 2013, Pages

  Samtleben, Samira ^a   Jaepel, Juliane ^a,b   Fecher, Caroline ^a   Andreska, Thomas ^a   Rehberg, Markus ^c   Blum, Robert ^a  

^a UNIVERSITY OF WÜRZBURG   (Germany)

^b MAX PLANCK INSTITUTE OF NEUROBIOLOGY   (Germany)

^c LUDWIG MAXIMILIANS UNIVERSITY   (Germany)

Author keywords

Anatomy; Animal model; Biochemistry; Bioengineering; Biomedical engineering; Ca2+; Calcium imaging; Calcium indicator; Calcium signaling; Calcium store; Cell culture; Cells; Cellular biology; Endoplasmic reticulum; ER; Imaging; Issue 75; Medicine; Metabotropic signaling; Molecular biology; Mouse; Neurobiology; Neurons; Neuroscience; Physiology; Surgery; Targeted esterase induced dye loading; Virology

Indexed keywords

CALCIUM; CARBOXYLESTERASE; FLUORESCENT DYE; PHOTOPROTEIN; RECOMBINANT PROTEIN; RED FLUORESCENT PROTEIN;

ANIMAL; ARTICLE; BIOSYNTHESIS; BRAIN CORTEX; CHEMISTRY; CYTOLOGY; ENDOPLASMIC RETICULUM; FLUORESCENCE IMAGING; GENE VECTOR; GENETICS; GLIA; HEK293 CELL LINE; HELA CELL LINE; HIPPOCAMPUS; HUMAN; LENTIVIRINAE; METABOLISM; METHODOLOGY; MOUSE; NERVE CELL;

ANIMALS; CALCIUM; CARBOXYLESTERASE; CEREBRAL CORTEX; ENDOPLASMIC RETICULUM; FLUORESCENT DYES; GENETIC VECTORS; HEK293 CELLS; HELA CELLS; HIPPOCAMPUS; HUMANS; LENTIVIRUS; LUMINESCENT PROTEINS; MICE; NEUROGLIA; NEURONS; OPTICAL IMAGING; RECOMBINANT PROTEINS;

EID: 84877777002     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/50317     Document Type: Article

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