Live cell calcium imaging combined with siRNA mediated gene silencing identifies Ca2+ leak channels in the ER membrane and their regulatory mechanisms

Journal of Visualized Experiments

Volumn , Issue 53, 2011, Pages

  Lang, Sven ^a   Schäuble, Nico ^a   Cavalié, Adolfo ^a   Zimmermann, Richard ^a  

^a SAARLAND UNIVERSITY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 80355132752     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/2730     Document Type: Article

References (13)

1. Clapham, D.E. Calcium signalling. Cell 131, 1047-1058 (2007).
2. 2+ leak from the endoplasmic reticulum of pancreatic acinar cells. J. Biol. Chem. 277, 26479-26485 (2002).
3. Van Coppenolle, F., et al. Ribosome-translocon complex mediates calcium leakage from endoplasmic reticulum stores. J. Cell Sci. 117, 4135-4142 (2004).
4. Flourakis, M., et al. Passive calcium leak via translocon is a first step for iPLA2-pathway regulated store operated channel activation. FASEB J. 20, 1215-1217 (2006).
5. 2+ leak from the endoplasmic reticulum: a mechanistic study on rat liver microsomes. Arch. Biochem. Biophys. 462, 115-121 (2007).
6. 2+ release via the ER translocon activates store operated calcium entry. Pflugers Arch. Eur. J. Physiol. 453, 797-808 (2007).
7. 2+ leak in proliferating vascular smooth muscle cells. Am. J. Physiol. Heart Circ. Physiol. 296, H910-H916 (2009).
8. 2+ leak channels, a function disrupted by familial Alzheimer's disease-linked mutations. Cell 126, 981-993 (2006).
9. Hamman, B.D., Hendershot, L.M., & Johnson, A.E. BiP maintains the permeability barrier of the ER membrane by sealing the lumenal end of the translocon pore before and early in translocation. Cell 92, 747-758 (1998).
10. Alder, N.N., Shen, Y., Brodsky, J.L., Hendershot, L.M., & Johnson, A.E. The molecular mechanism underlying BiP-mediated gating of the Sec61 translocon of the endoplasmic reticulum. J. Cell Biol. 168, 389-399 (2005).
11. 2+ exchangers in mast cells. J. Immunol. 174, 119-130 (2005).
12. 2+-selective ion channels. J. Biol. Chem. 284, 34423-34432 (2009).
13. 2+ leakage from the endoplasmic reticulum. EMBO J. 30, 17-31 (2011).