Quantitative quenching evaluation and direct intracellular metabolite analysis in Penicillium chrysogenum

Analytical Biochemistry

Volumn 438, Issue 1, 2013, Pages 47-52

  Meinert, Sabine ^a,b   Rapp, Sina ^a   Schmitz, Katja ^c   Noack, Stephan ^c   Kornfeld, Georg ^a   Hardiman, Timo ^a  

^a NOVARTIS PHARMA AG   (Switzerland)

^b UNIVERSITY OF INNSBRUCK   (Austria)

^c FORSCHUNGSZENTRUM JÜLICH   (Germany)

Author keywords

Intracellular metabolite analysis; Leakage; Penicillium chrysogenum; Quenching

Indexed keywords

BIOMOLECULES; CYTOLOGY; LEAKAGE (FLUID); METABOLIC ENGINEERING; METABOLISM; QUENCHING;

ENGINEERING METHODOLOGY; INDUSTRIAL PROCESSS; INDUSTRIAL PRODUCTION; INTRACELLULAR METABOLITES; METABOLITE ANALYSIS; PENICILLIUM CHRYSOGENUM; QUENCHING EFFICIENCY; SAMPLING PROCEDURES;

METABOLITES;

CARBON 13; DIHYDROXYACETONE PHOSPHATE; FRUCTOSE 6 PHOSPHATE; GLUCOSE 6 PHOSPHATE; GLYCERALDEHYDE 3 PHOSPHATE;

ARTICLE; BATCH PROCESS; CENTRIFUGATION; CITRIC ACID CYCLE; CONTROLLED STUDY; ENZYME ACTIVITY; FED BATCH REACTOR; FILTRATION; FUNGAL BIOMASS; FUNGAL MEMBRANE; FUNGAL METABOLISM; FUNGUS CULTURE; GAS FLOW; GLYCOLYSIS; INDUSTRIAL PRODUCTION; METABOLIC ENGINEERING; METABOLITE; METABOLOMICS; NONHUMAN; PENICILLIUM CHRYSOGENUM; PENTOSE PHOSPHATE CYCLE; PRIORITY JOURNAL;

PENICILLIUM CHRYSOGENUM;

EID: 84877027832     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2013.03.021     Document Type: Article

References (37)

1. J. Thykaer, and J. Nielsen Metabolic engineering of beta-lactam production Metab. Eng. 5 2003 56 69
2. N. Keller The fungal treasure chest: spore origins? Fungal Biol. Rev. 25 2011 73 77
3. M. McIntyre, C. Muller, J. Dynesen, and J. Nielsen Metabolic engineering of the morphology of Aspergillus Adv. Biochem. Eng. Biotechnol. 73 2001 103 128
4. T. Wucherpfennig, K.A. Kiep, H. Driouch, C. Wittmann, and R. Krull Morphology and rheology in filamentous cultivations Adv. Appl. Microbiol. 72 2010 89 136
5. C. Chassagnole, N. Noisommit-Rizzi, J.W. Schmid, K. Mauch, and M. Reuss Dynamic modeling of the central carbon metabolism of Escherichia coli Biotechnol. Bioeng. 79 2002 53 73
6. T. Hardiman, H. Meinhold, J. Hofmann, J.C. Ewald, M. Siemann-Herzberg, and M. Reuss Prediction of kinetic parameters from DNA-binding site sequences for modeling global transcription dynamics in Escherichia coli Metab. Eng. 12 2010 196 211
7. U. Nasution, W.M. van Gulik, A. Proell, W.A. van Winden, and J.J. Heijnen Generating short-term kinetic responses of primary metabolism of Penicillium chrysogenum through glucose perturbation in the bioscope mini reactor Metab. Eng. 8 2006 395 405
8. M. Oldiges, and R. Takors Applying metabolic profiling techniques for stimulus-response experiments: chances and pitfalls Adv. Biochem. Eng. Biotechnol. 92 2005 173 196
9. J. Schaub, and M. Reuss In vivo dynamics of glycolysis in Escherichia coli shows need for growth-rate dependent metabolome analysis Biotechnol. Prog. 24 2008 1402 1407
10. U. Theobald, W. Mailinger, M. Reuss, and M. Rizzi In vivo analysis of glucose induced fast changes in yeast adenine-nucleotide pool applying a rapid sampling technique Anal. Biochem. 214 1993 31 37
11. W.M. van Gulik, W.T.A.M. de Laat, J.L. Vinke, and J.J. Heijnen Application of metabolic flux analysis for the identification of metabolic bottlenecks in the biosynthesis of penicillin-G Biotechnol. Bioeng. 68 2000 602 618
12. 13C-labeled glucose Biotechnol. Bioeng. 68 2000 652 659
13. 13C- labeling-based metabolic flux analysis and l-lysine production Metab. Eng. 5 2003 96 107
14. T. Hardiman, K. Lemuth, M.A. Keller, M. Reuss, and M. Siemann-Herzberg Topology of the global regulatory network of carbon limitation in Escherichia coli J. Biotechnol. 132 2007 359 374
15. 13C-MFA: a comparison using a consistent dataset J. Biotechnol. 154 2011 179 190
16. M. Wellerdiek, D. Winterhoff, W. Reule, J. Brandner, and M. Oldiges Metabolic quenching of Corynebacterium glutamicum: efficiency of methods and impact of cold shock Bioprocess Biosyst. Eng. 32 2009 581 592
17. S.G. Villas-Boas, J. Hojer-Pedersen, M. Akesson, J. Smedsgaard, and J. Nielsen Global metabolite analysis of yeast: evaluation of sample preparation methods Yeast 22 2005 1155 1169
18. J. Schaub, C. Schiesling, M. Reuss, and M. Dauner Integrated sampling procedure for metabolome analysis Biotechnol. Prog. 22 2006 1434 1442
19. H. Taymaz-Nikerel, M. de Mey, C. Ras, A. Pierick, R.M. Seifar, J.C. van Dam, J.J. Heijnen, and W.M. van Gulik Development and application of a differential method for reliable metabolome analysis in Escherichia coli Anal. Biochem. 386 2009 9 19
20. R.D. Douma, L.P. de Jonge, C.T.H. Jonker, R.M. Seifar, J.J. Heijnen, and W.M. van Gulik Intracellular metabolite determination in the presence of extracellular abundance: application to the penicillin biosynthesis pathway in Penicillium chrysogenum Biotechnol. Bioeng. 107 2010 105 115
21. U. Nasution, W.M. van Gulik, R.J. Kleijn, W.A. van Winden, A. Proell, and J.J. Heiinen Measurement of intracellular metabolites of primary metabolism and adenine nucleotides in chemostat cultivated Penicillium chrysogenum Biotechnol. Bioeng. 94 2006 159 166
22. 13C-labeled gluconate tracing as a direct and accurate method for determining the pentose phosphate pathway split ratio in Penicillium chrysogenum Appl. Environ. Microbiol. 72 2006 4743 4754
23. U. Nasution, W.M. van Gulik, C. Ras, A. Proell, and J.J. Heijnen A metabolome study of the steady-state relation between central metabolism, amino acid biosynthesis and penicillin production in Penicillium chrysogenum Metab. Eng. 10 2008 10 23
24. L.P. de Jonge, R.D. Douma, J.J. Heijnen, and W.M. van Gulik Optimization of cold methanol quenching for quantitative metabolomics of Penicillium chrysogenum Metabolomics 8 2012 727 735
25. B. Gonzalez, J. Francois, and M. Renaud A rapid and reliable method for metabolite extraction in yeast using boiling buffered ethanol Yeast 13 1997 1347 1355
26. A.B. Canelas, A. ten Pierick, C. Ras, R.M. Seifar, J.C. van Dam, and W.M. van Gulik Quantitative evaluation of intracellular metabolite extraction techniques for yeast metabolomics Anal. Chem. 81 2009 7379 7389
27. W. de Koning, and K. van Dam A method for the determination of changes of glycolytic metabolites in yeast on a subsecond time scale using extraction at neutral pH Anal. Biochem. 204 1992 118 123
28. 13C-labeling data FEMS Yeast Res. 7 2007 216 231
29. H.C. Lange, M. Eman, G. van Zuijlen, D. Visser, J.C. van Dam, and J. Frank Improved rapid sampling for in vivo kinetics of intracellular metabolites in Saccharomyces cerevisiae Biotechnol. Bioeng. 75 2001 406 415
30. C. Wittmann, J.O. Kromer, P. Kiefer, T. Binz, and E. Heinzle Impact of the cold shock phenomenon on quantification of intracellular metabolites in bacteria Anal. Biochem. 327 2004 135 139
31. C.J. Bolten, P. Kiefer, F. Letisse, J.C. Portais, and C. Wittmann Sampling for metabolome analysis of microorganisms Anal. Chem. 79 2007 3843 3849
32. G.J.G. Ruijter, and J. Visser Determination of intermediary metabolites in Aspergillus niger J. Microbiol. Methods 25 1996 295 302
33. A.B. Canelas, C. Ras, A. ten Pierick, J.C. van Dam, J.J. Heijnen, and W.M. van Gulik Leakage-free rapid quenching technique for yeast metabolomics Metabolomics 4 2008 226 239
34. J. Lein Z. Vanek, Z. Hostalek, Overproduction of Microbial Metabolites: Strain Improvement and Process Control Strategies 1986 Butterworth Stoneham, MA 105 140
35. B. Luo, K. Groenke, R. Takors, C. Wandrey, and M. Oldiges Simultaneous determination of multiple intracellular metabolites in glycolysis, pentose phosphate pathway and tricarboxylic acid cycle by liquid chromatography-mass spectrometry J. Chromatogr. A 1147 2007 153 164
36. M. Dell'mour, L. Jaitz, E. Oburger, M. Puschenreiter, G. Koellensperger, and S. Hann Hydrophilic interaction LC combined with electrospray MS for highly sensitive analysis of underivatized amino acids in rhizosphere research J. Sep. Sci. 33 2010 911 922
37. 13C cell extract of Pichia pastoris - a powerful tool for evaluation of sample preparation in metabolomics J. Sep. Sci. 35 2012 3091 3105