Metabolic quenching of corynebacterium glutamicum: Efficiency of methods and impact of cold shock

Bioprocess and Biosystems Engineering

Volumn 32, Issue 5, 2009, Pages 581-592

  Wellerdiek, Max ^a   Winterhoff, Dajana ^a   Reule, Waldemar ^c   Brandner, Jürgen ^b   Oldiges, Marco ^a  

^a FORSCHUNGSZENTRUM JÜLICH   (Germany)

^b KARLSRUHE INSTITUTE OF TECHNOLOGY   (Germany)

^c Hochschule Furthwangen   (Germany)

Author keywords

Metabolome analysis; Metabolomics; Methanol quenching; Microstructure heat exchanger; Rapid sampling

Indexed keywords

AUTOMATED SAMPLING; CELL SUSPENSION; COLD SHOCK; CONVERSION RATES; CORYNEBACTERIUM GLUTAMICUM; LEAKAGE EFFECTS; METABOLIC INACTIVATION; METABOLOME ANALYSIS; METABOLOMICS; MICROSTRUCTURE HEAT EXCHANGER; RAPID QUENCHING TECHNIQUE; RAPID SAMPLING; ROBUST TECHNIQUE; SAMPLE PROCESSING; TEMPERATURE SHIFT; TIME-SCALES; TURNOVER RATE;

BIOCHEMISTRY; CELL CULTURE; CELL MEMBRANES; HEAT EXCHANGERS; METABOLISM; METHANOL; MICROSTRUCTURE;

QUENCHING;

METHANOL; SILICON;

APPARATUS; ARTICLE; CELL SUSPENSION; CITRIC ACID CYCLE; COLD STRESS; COOLING; CORYNEBACTERIUM GLUTAMICUM; GLYCOLYSIS; HEAT EXCHANGE; METABOLIC ENGINEERING; METABOLOMICS; MICROBIAL METABOLISM; NONHUMAN; PENTOSE PHOSPHATE CYCLE; PRIORITY JOURNAL; REPRODUCIBILITY; SYSTEMS BIOLOGY; TEMPERATURE; TURNOVER TIME;

ADAPTATION, PHYSIOLOGICAL; BACTERIAL PROTEINS; CELL CULTURE TECHNIQUES; COLD TEMPERATURE; CORYNEBACTERIUM GLUTAMICUM; EQUIPMENT DESIGN; EQUIPMENT FAILURE ANALYSIS; HEAT-SHOCK PROTEINS; HEAT-SHOCK RESPONSE;

CORYNEBACTERIUM GLUTAMICUM;

EID: 68349130318     PISSN: 16157591     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00449-008-0280-y     Document Type: Article

References (35)

1. VF Wendisch M Bott J Kalinowski M Oldiges W Wiechert 2006 Emerging Corynebacterium glutamicum systems biology J Biotechnol 124 74 92
2. M Oldiges S Lütz S Pflug K Schroer N Stein C Wiendahl 2007 Metabolomics: current state and evolving methodologies and tools Appl Microbiol Biotechnol 76 495 511
3. U Schäfer W Boos R Takors D Weuster-Botz 1999 Automated sampling device for monitoring intracellular metabolite dynamics Anal Biochem 270 88 96
4. C Chassagnole N Noisommit-Rizzi JW Schmid K Mauch M Reuss 2002 Dynamic modeling of the central carbon metabolism of Escherichia coli Biotechnol Bioeng 79 53 73
5. M Oldiges M Kunze D Degenring GA Sprenger R Takors 2004 Stimulation, monitoring, and analysis of pathway dynamics by metabolic profiling in the aromatic amino acid pathway Biotechnol Prog 20 1623 1633
6. U Theobald W Mailinger M Baltes M Rizzi M Reuss 1997 In vivo analysis of metabolic dynamics in Saccharomyces cerevisiae.1. Experimental observations Biotechnol Bioeng 55 305 316 (Pubitemid 27291199)
7. D Visser GA van Zuylen JC van Dam MR Eman A Proll C Ras L Wu WM van Gulik JJ Heijnen 2004 Analysis of in vivo kinetics of glycolysis in aerobic Saccharomyces cerevisiae by application of glucose and ethanol pulses Biotechnol Bioeng 88 157 167
8. T Soga Y Ohashi Y Ueno H Naraoka M Tomita T Nishioka 2003 Quantitative metabolome analysis using capillary electrophoresis mass spectrometry J Proteome Res 2 488 494
9. MJ van der Werf KM Overkamp B Muilwijk MM Kök BJC der Vat RH Jellema L Coulier T Hankemeier 2008 Comprehensive analysis of the metabolome of Pseudomonas putida S12 grown on different carbon sources Mol Biosyst 4 315 327
10. JB Magnus D Hollwedel M Oldiges R Takors 2006 Monitoring and modeling of the reaction dynamics in the valine/leucine synthesis pathway in Corynebacterium glutamicum Biotechnol Prog 22 1071 1083
11. S Strelkov M von Elstermann D Schomburg 2004 Comprehensive analysis of metabolites in Corynebacterium glutamicum by gas chromatography/mass spectrometry Biol Chem 385 853 861
12. O Fiehn 2002 Metabolomics-the link between genotypes and phenotypes Plant Mol Biol 48 155 171
13. W Dekoning K Vandam 1992 A method for the determination of changes of glycolytic metabolites in yeast on a subsecond time scale using extraction at neutral pH Anal Biochem 204 118 123
14. D Visser GA van Zuylen JC van Dam A Oudshoorn MR Eman C Ras WM van Gulik J Frank GWK van Dedem JJ Heijnen 2002 Rapid sampling for analysis of in vivo kinetics using the BioScope: a system for continuous-pulse experiments Biotechnol Bioeng 79 674 681
15. S Buziol I Bashir A Baumeister W Claassen N Noisommit-Rizzi W Mailinger M Reuss 2002 New bioreactor-coupled rapid stopped-flow sampling technique for measurements of metabolite dynamics on a subsecond time scale Biotechnol Bioeng 80 632 636
16. J Schaub C Schiesling M Reuss M Dauner 2006 Integrated sampling procedure for metabolome analysis Biotechnol Prog 22 1434 1442
17. C Cordeiro AF Freire 1996 Methylglyoxal assay in cells as 2-methylquinoxaline using 1,2-diaminobenzene as derivatizing reagent Anal Biochem 234 221 224
18. D WeusterBotz 1997 Sampling tube device for monitoring intracellular metabolite dynamics Anal Biochem 246 225 233
19. C Wittmann JO Krömer P Kiefer T Binz E Heinzle 2004 Impact of the cold shock phenomenon on quantification of intracellular metabolites in bacteria Anal Biochem 327 135 139
20. JJ Brandner E Anurjew L Bohn E Hansjosten T Henning U Schygulla A Wenka K Schubert 2006 Concepts and realization of microstructure heat exchangers for enhanced heat transfer Exp Therm Fluid Sci 30 801 809
21. C Schultz A Niebisch L Gebel M Bott 2007 Glutamate production by Corynebacterium glutamicum: dependence on the oxoglutarate dehydrogenase inhibitor protein OdhI and protein kinase PknG Appl Microbiol Biotechnol 76 691 700
22. B Blombach ME Schreiner J Holatko T Bartek M Oldiges BJ Eikmanns 2007 (l)-Valine production with pyruvate dehydrogenase complex-deficient Corynebacterium glutamicum Appl Environ Microbiol 73 2079 2084 (Pubitemid 46649496)
23. T Bartek P Makus B Klein S Lang M Oldiges 2008 Influence of l-isoleucine and pantothenate auxotrophy for l-valine formation in Corynebacterium glutamicum revisited by metabolome analyses Bioprocess Biosyst Eng 31 217 225
24. B Luo K Grönke R Takors C Wandrey M Oldiges 2007 Simultaneous determination of multiple intracellular metabolites in glycolysis, pentose phosphate pathway and tricarboxylic acid cycle by liquid chromatography-mass spectrometry J Chromatogr A 1147 153 164
25. K Nöh K Grönke B Luo R Takors M Oldiges W Wiechert 2007 Metabolic flux analysis at ultra short time scale: Isotopically non-stationary C-13 labeling experiments J Biotechnol 129 249 267
26. Oldiges M. 2005. Dissertation thesis: Metabolomanalyse zur Untersuchung der Dynamik im Aromatenbiosyntheseweg in l-Phenylalanin Produzenten von Escherichia coli. Lebenswissenschaften/Life Science, ISBN 3-89336-380-7
27. B Zelic D Vasic-Racki C Wandrey R Takors 2004 Modeling of the pyruvate production with Escherichia coli in a fed-batch bioreactor Bioprocess Biosyst Eng 26 249 258
28. C Wiendahl JJ Brandner C Küppers B Luo U Schygulla T Noll M Oldiges 2007 A microstructure heat exchanger for quenching the metabolism of mammalian cells Chem Eng Technol 30 322 328
29. CJ Bolten P Kiefer F Letisse JC Portais C Wittmann 2007 Sampling for metabolome analysis of microorganisms Anal Chem 79 3843 3849
30. NBS Jensen KV Jokumsen J Villadsen 1999 Determination of the phosphorylated sugars of the Embden-Meyerhoff-Parnas pathway in Lactococcus lactis using a fast sampling technique and solid phase extraction Biotechnol Bioeng 63 356 362
31. U Theobald W Mailinger M Reuss M Rizzi 1993 In vivo analysis of glucose-induced fast changes in yeast adenine nucleotide pool applying a rapid sampling technique Anal Biochem 214 31 37
32. J Plassmeier A Barsch M Persicke K Niehaus J Kalinowski 2007 Investigation of central carbon metabolism and the 2-methylcitrate cycle in Corynebacterium glutamicum by metabolic profiling using gas chromatography-mass spectrometry J Biotechnol 130 354 363
33. HC Lange M Eman G van Zuijlen D Visser JC van Dam J Frank MJT de Mattos JJ Heijnen 2001 Improved rapid sampling for in vivo kinetics of intracellular metabolites in Saccharomyces cerevisiae Biotechnol Bioeng 75 406 415
34. J Hiller E Franco-Lara V Papaioannou D Weuster-Botz 2007 Fast sampling and quenching procedures for microbial metabolic profiling Biotechnol Lett 29 1161 1167
35. WeusterBotz D (1999) Habilitation thesis: Die Rolle der Reaktionstechnik in der mikrobiellen Verfahrensentwicklung, Jülich. Lebenswissenschaften/ Life Sciences, ISBN 3-89336-245-2