A high sensitive nested PCR for toxoplasma gondii detection in animal and food samples

Journal of Microbial and Biochemical Technology

Volumn 5, Issue 2, 2013, Pages 39-41

  Vitale, Maria ^a   Galluzzo, Paola ^a   Currò, Vittoria ^a   Gozdzik, Kataryna ^b   Schillaci, Domenico ^c   Presti, Vincenzo Di Marco Lo ^a  

^a ISTITUTO ZOOPROFILATTICO SPERIMENTALE DELLA SICILIA A MIRRI   (Italy)

^b W STEFA SKI INSTITUTE OF PARASITOLOGY   (Poland)

^c UNIVERSITY OF PALERMO   (Italy)

Author keywords

Neospora caninum; Nested PCR; Restriction fragments length polymorphism; Toxoplasma gondii

Indexed keywords

DNA; DNA 18S; DNA 28S; UNCLASSIFIED DRUG;

ABORTION; AMPLICON; ANIMAL HEALTH; ARTICLE; CATTLE; CONTROLLED STUDY; DILUTION; DNA DETERMINATION; FOOD ANALYSIS; GENE AMPLIFICATION; GENOME SIZE; LIMIT OF DETECTION; MEAT; MICROBIAL MORPHOLOGY; NEOSPORA CANINUM; NESTED POLYMERASE CHAIN REACTION; NONHUMAN; NUCLEIC ACID ANALYSIS; PARASITE EXAMINATION; PARASITE TRANSMISSION; REAL TIME POLYMERASE CHAIN REACTION; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; SENSITIVITY ANALYSIS; TOXOPLASMA GONDII; VETERINARY MEDICINE;

ANIMALIA; BOS; NEOSPORA CANINUM; TOXOPLASMA GONDII;

EID: 84877023442     PISSN: None     EISSN: 19485948     Source Type: Journal    
DOI: 10.4172/1948-5948.1000097     Document Type: Article

References (16)

1. Jones JL, Dubey JP (2010) Waterborne toxoplasmosis--recent developments. Exp Parasitol 124: 10-25.
2. Dubey JP (2010) Toxoplasmosis of animals and humans. CRC Press, Boca Raton, FL.
3. Cook AJ, Gilbert RE, Buffolano W, Zufferey J, Petersen E, et al. (2000) Sources of toxoplasma infection in pregnant women: European multicentre case-control study. European Research Network on Congenital Toxoplasmosis. BMJ 321: 142-147.
4. Vitale M, Di Marco Lo Presti V (2013) Food Safety or Typical Dishes? Toxoplasma gondii and Educational Preventive Campaign. Foodborne Pathog Dis 10: 196.
5. Guay JM, Huot A, Gagnon S, Tremblay A, Levesque RC (1992) Physical and genetic mapping of cloned ribosomal DNA from Toxoplasma gondii: primary and secondary structure of the 5S gene. Gene 114: 165-171.
6. Goździk K, Cabaj W (2007) Characterization of the first Polish isolate of Neospora caninum from cattle. ActaParasitol 52: 295-297.
7. Jauregui LH, Higgins J, Zarlenga D, Dubey JP, Lunney JK (2001) Development of a real-time PCR assay for detection of Toxoplasma gondii in pig and mouse tissues. J Clin Microbiol 39: 2065-2071.
8. Dubey JP, Carpenter JL, Speer CA, Topper MJ, Uggla A (1988) Newly recognized fatal protozoan disease of dogs. J Am Vet Med Assoc 192: 1269-1285.
9. Dubey JP, Schares G, Ortega-Mora L (2007) Epidemiology and control of neosporosis and Neospora caninum. ClinMicrobiol Rev 20: 323-367.
10. Bastien P (2002) Molecular diagnosis of toxoplasmosis. Trans R Soc Trop Med Hyg 96 Suppl 1:S205-215.
11. Switaj K, Master A, Skrzypczak M, Zaborowski P (2005) Recent trends in molecular diagnostics for Toxoplasma gondii infections. Clin Microbiol Infect 11:170-176.
12. Reischl U, Bretagne S, Krüger D, Ernault P, Costa JM (2003) Comparison of two DNA targets for the diagnosis of Toxoplasmosis by real-time PCR using fluorescence resonance energy transfer hybridization probes. BMC Infect Dis 3: 7.
13. Calderaro A, Piccolo G, Gorrini C, Peruzzi S, Zerbini L, et al. (2006) Comparison between two real-time PCR assays and a nested-PCR for the detection of Toxoplasma gondii. Acta Biomed 77: 75-80.
14. Hierl T, Reischl U, Lang P, Hebart H, Stark M, et al. (2004) Preliminary evaluation of one conventional nested and two real-time PCR assays for the detection of Toxoplasma gondii in immunocompromised patients. J Med Microbiol 53: 629-632.
15. Drago L, Lombardi A, De Vecchi E, Giuliani G, Bartolone R, et al. (2004) Comparison of nested PCR and real time PCR of Herpesvirus infections of central nervous system in HIV patients. BMC Infect Dis 4: 55.
16. Bastien P, Procop GW, Reischl U (2008) Quantitative real-time PCR is not more sensitive than "conventional" PCR. J Clin Microbiol 46: 1897-1900.