Comparison between two real-time PCR assays and a nested-PCR for the detection of Toxoplasma gondii

Acta Biomedica de l'Ateneo Parmense

Volumn 77, Issue 2, 2006, Pages

  Calderaro, Adriana ^a   Piccolo, Giovanna ^a   Gorrini, Chiara ^a   Peruzzi, Simona ^a   Zerbini, Laura ^a   Bommezzadri, Simona ^a   Dettori, Giuseppe ^a   Chezzi, Carla ^a  

^a UNIVERSITY OF PARMA   (Italy)

Author keywords

Nested PCR; Real time PCRs; Toxoplasma gondii

Indexed keywords

ARTICLE; COMPARATIVE STUDY; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; FEMALE; GENE SEQUENCE; HUMAN; HUMAN TISSUE; IMMUNE DEFICIENCY; LABORATORY DIAGNOSIS; METHODOLOGY; NESTED POLYMERASE CHAIN REACTION; PARASITE IDENTIFICATION; POLYMERASE CHAIN REACTION; PREGNANT WOMAN; REAL TIME POLYMERASE CHAIN REACTION; RNA GENE; SENSITIVITY AND SPECIFICITY; TOXOPLASMA GONDII; TOXOPLASMOSIS;

ADULT; ANIMALS; DNA, PROTOZOAN; FEMALE; GENES, PROTOZOAN; HUMANS; INFANT, NEWBORN; MALE; POLYMERASE CHAIN REACTION; PREGNANCY; SENSITIVITY AND SPECIFICITY; TOXOPLASMA; TOXOPLASMOSIS;

EID: 33845366750     PISSN: 03924203     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (19)

1. th ed. The WB Saunders Co., Philadelphia, Pa. 1995; 140-267.
2. Luft BJ, Remington JS. Toxoplasmic encephalitis in AIDS. Clin Infect Dis 1992; 15: 211-22.
3. Israelski DM, Remington JS. Toxoplasmosis in the non-AIDS immunocompromised host. Curr Clin Top Infect Dis 1993; 13: 322-56.
4. Wong S, Remington JS. Toxoplasmosis in pregnancy. Clin Infect Dis 1994; 18: 853-62.
5. Bretagne S, Costa JM, Vidaud M, Tran J, Nhieu V, Fleury-Feith J. Detection of Toxoplasma gondii by competitive DNA amplification of bronchoalveolar lavage samples. J Infect Dis 1993; 168: 1585-8.
6. Hohfeld P, Daffos F, Costa JM, Thulliez P, Forestier F, Vidaud M. Prenatal diagnosis of congenital toxoplasmosis with a polymerase-chain-reaction test on amniotic fluid. New Eng J Med 1994; 331: 695-9.
7. Kupferschmidt O, Krüger D, Held TK, Ellerbrok H, Siegert W, Janitschke K. Quantitative detection of Toxoplasma gondii DNA in human body fluids by TaqMan polymerase chain reaction. Clin Microbiol Infect 2001; 7: 120-4.
8. James GS, Sinttchenko VG, Dickeson, DJ, Gilbert GL. Comparison of cell culture, mouse inoculation, and PCR for detection of Toxoplasma gondii: effects of storage conditions on sensitivity. J Clin Microbiol 1996; 34: 1572-5.
9. Buchbinder S, Blatz R, Rodloff AC. Comparison of real-time PCR detection methods for B1 and P30 genes of Toxoplasma gondii. Diagn Microbiol Infec Dis 2003; 45: 269-71.
10. Simon A, Labalette P, Ordinaire I, et al. Use of fluorescence resonance energy transfer hybridization probes to evaluate quantitative Real-time PCR for diagnosis of ocular toxoplasmosis. J Clin Microbiol 2004; 42: 3681-5.
11. Bastien P. Molecular diagnosis of toxoplasmosis. Transactions of the Royal Society of Tropical Medicine and Hygiene 2002; 96: S1/205-S1/215.
12. Burg JL, Grover CM, Pouletty P, Boothroyd JC. Direct and sensitive detection of a pathogenic protozoan, Toxoplasma gondii, by polymerase chain reaction. J Clin Microbiol 1989; 27: 1787-92.
13. Homan WL, Vercammen M, De Braekeleer J, Verschueren H. Identification of a 200- to 300-fold repetitive 529 bp DNA fragment in Toxoplasma gondii, and its use for diagnostic and quantitative PCR. Int J Parasitol 2000; 30: 69-75.
14. Reischl U, Bretagne S, Krüger D, Ernault P, Costa JM. Comparison of two DNA targets for the diagnosis of toxoplasmosis by real-time PCR using fluorescence energy transfer hybridization probes. BMC Infectious Diseases 2003; 3: 7-15.
15. Jauregui LH, Higgins J, Zarlenga D, Dubey JP, Lunney JK. Development of a real-time PCR assay for detection of Toxoplasma gondii in pig and mouse tissue. J Clinl Microbiol 2001; 6: 2065-71.
16. Lachaud L, Dereure J, Chabbert E, et al. Optimized PCR using patient blood samples for diagnosis and follow-up of visceral leishmaniosis with special reference to AIDS. J Clin Microbiol 2000; 38: 236-40.
17. Cornelissen AWCA, Overdulve JP, Van Der Ploeg M. Determination of nuclear DNA of five eucoccidian parasites, Isospora (Toxoplasma) gondii, Sarcocystis cruzi, Eimeria tenella, E. acervulina and Plasmodium berghei, with special reference to gametogenesis and meiosis I. (T.) gondii. Parasitology 1984; 88: 531-53.
18. Hierl T, Reischl U, Lang P, et al. Preliminary evaluation of one conventional nested and two real-time PCR assays for the detection of Toxoplasma gondii in immunocompromised patients. J Med Microbiol 2004; 53: 629-32.
19. Hurtado A, Aduriz G, Moreno B, Barandika J, Garcia-Perez AL. Single Tube nested PCR for the detection of Toxoplasma gondii in fetal tissues from naturally aborted ewes. Vet Parasitol. 2001 3; 102: 17-27.