Purification, characterization, and gene cloning of a cold-adapted thermolysin-like protease from Halobacillus sp. SCSIO 20089

Journal of Bioscience and Bioengineering

Volumn 115, Issue 6, 2013, Pages 628-632

  Yang, Jian ^a,b   Li, Jie ^a   Mai, Zhimao ^a,b   Tian, Xinpeng ^a   Zhang, Si ^a,b  

^a SOUTH CHINA SEA INSTITUTE OF OCEANOLOGY   (China)

^b UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

Author keywords

Cold active enzyme; Enzyme purification; Genome walking; Marine sediment; Protease

Indexed keywords

COLD-ACTIVE ENZYMES; ENZYME PURIFICATION; GENOME-WALKING; MARINE SEDIMENTS; PROTEASE;

ACTIVATION ENERGY; AMINO ACIDS; ENZYMES; GENE ENCODING; INDUSTRIAL APPLICATIONS; INDUSTRIAL RESEARCH; SUBMARINE GEOLOGY;

PURIFICATION;

BACTERIAL RNA; PROTEINASE; RNA 16S; THERMOLYSIN;

AMINO ACID SEQUENCE; ARTICLE; BACTERIAL GROWTH; BACTERIAL STRAIN; BACTERIUM CULTURE; BACTERIUM ISOLATION; BINDING SITE; CELL PH; COLD ACCLIMATIZATION; ENZYME ACTIVATION; ENZYME ACTIVE SITE; ENZYME ACTIVITY; HALOBACILLUS; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN DEGRADATION; PROTEIN PURIFICATION; SEQUENCE ANALYSIS;

ADAPTATION, BIOLOGICAL; AMINO ACID SEQUENCE; CLONING, MOLECULAR; COLD TEMPERATURE; HALOBACILLUS; METALLOENDOPEPTIDASES; MOLECULAR SEQUENCE DATA; SUBSTRATE SPECIFICITY;

HALOBACILLUS SP.;

EID: 84876988510     PISSN: 13891723     EISSN: 13474421     Source Type: Journal    
DOI: 10.1016/j.jbiosc.2012.12.013     Document Type: Article

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