Cloning, expression, and identification of a novel extracellular cold-adapted alkaline protease gene of the marine bacterium strain YS-80-122

Applied Biochemistry and Biotechnology

Volumn 162, Issue 5, 2010, Pages 1497-1505

  Wang, Fang ^a   Hao, Jianhua ^a   Yang, Chengye ^a   Sun, Mi ^a  

^a YELLOW SEA FISHERIES RESEARCH INSTITUTE   (China)

Author keywords

Alkaline protease; Cold adapted; lupA; Metalloprotease; Serralysin

Indexed keywords

ALKALINE PROTEASE; COLD-ADAPTED; LUPA; METALLO-PROTEASE; SERRALYSIN;

AMINO ACIDS; BACTERIA; CLONING; ESCHERICHIA COLI; GENE ENCODING; GENE EXPRESSION; ORGANIC ACIDS; PROTEINS; PURIFICATION;

ENZYME ACTIVITY;

ALKALINE PROTEINASE; METALLOPROTEINASE; RECOMBINANT PROTEIN; SIGNAL PEPTIDE;

AMINO ACID SEQUENCE; ARTICLE; COLD ACCLIMATIZATION; CONTROLLED STUDY; ESCHERICHIA COLI; EXTRACELLULAR SPACE; INVERSE POLYMERASE CHAIN REACTION; LUPA GENE; MARINE BACTERIUM; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PSEUDOMONAS; RAT; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY; STRUCTURAL GENE; WESTERN BLOTTING;

ADAPTATION, PHYSIOLOGICAL; AMINO ACID SEQUENCE; BACTERIA; BLOTTING, WESTERN; CHROMATOGRAPHY, GEL; CLONING, MOLECULAR; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; EXTRACELLULAR SPACE; GENES, BACTERIAL; METALLOENDOPEPTIDASES; MOLECULAR SEQUENCE DATA; RECOMBINANT PROTEINS; SEAWATER; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; PSEUDOMONAS SP. 'TAC II 18';

EID: 77955923960     PISSN: 02732289     EISSN: None     Source Type: Journal    
DOI: 10.1007/s12010-010-8927-y     Document Type: Article

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