Cloning, expression, and purification of pseudomonas aeruginosa keratinase in escherichia coli AD494(DE3)pLysS expression system

Journal of Agricultural and Food Chemistry

Volumn 57, Issue 9, 2009, Pages 3506-3511

  Lin, Hsin Hung ^a   Yin, Li Jung ^b   Jiang, Shann Tzong ^a,c  

^a NATIONAL TAIWAN OCEAN UNIVERSITY   (Taiwan)

^b NATIONAL KAOHSIUNG UNIVERSITY OF APPLIED SCIENCES   (Taiwan)

^c PROVIDENCE UNIVERSITY   (Taiwan)

Author keywords

Cloning; E. coli; Expression; Keratinase; Pseudomonas aeruginosa

Indexed keywords

CATION; ENZYME INHIBITOR; KERATINASE; PEPTIDE HYDROLASE; RECOMBINANT PROTEIN;

ARTICLE; BACTERIUM TRANSFORMATION; DRUG EFFECT; ENZYME ACTIVATION; ENZYME SPECIFICITY; ENZYME STABILITY; ENZYMOLOGY; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; PH; PSEUDOMONAS AERUGINOSA;

CATIONS; CLONING, MOLECULAR; ENZYME ACTIVATION; ENZYME INHIBITORS; ENZYME STABILITY; ESCHERICHIA COLI; GENE EXPRESSION; HYDROGEN-ION CONCENTRATION; PEPTIDE HYDROLASES; PSEUDOMONAS AERUGINOSA; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY; TRANSFORMATION, BACTERIAL;

ESCHERICHIA COLI; PSEUDOMONAS AERUGINOSA;

EID: 66149140273     PISSN: 00218561     EISSN: None     Source Type: Journal    
DOI: 10.1021/jf803752j     Document Type: Article

References (30)

1. Lin, X.; Kelemen, D. W.; Miller, E. S.; Shih, J. C. Nucleotide sequence and expression of kerA, the gene encoding a keratinolytic protease of Bacillus licheniformis PWD-1. Appl. Environ. Microbiol. 1995, 61 (4), 1469-1474.
2. Parry, D. A. D.; Crewther, W. G.; Fraser, R. O. B.; MacRae, T. P. Structure of R-keratin: structural implication of the amino acid sequence of the type I and type II chain segments. J. Mol. Biol. 1977, 113, 449-454.
3. Gupta, R.; Ramnani, P. Microbial keratinases and their prospective applications: an overview. Appl. Microbiol. Biotechnol. 2006, 70 (1), 21-33.
4. Yin, L. J.; Lee, J. H.; Jiang, S. T. Isolation of a keratinaseproducing bacterium and purification of its keratinase. J. Fish. Soc. Taiwan 2006, 33 (4), 377-390.
5. Stover, C. K.; Pham, X. Q.; Erwin, A. L.; Mizoguchi, S. D.; Warrener, P.; Hickey, M. J.; Brinkman, F. S.; Hufnagle, W. O.; Kowalik, D. J.; Lagrou, M.; Garber, R. L.; Goltry, L.; Tolentino, E.; Westbrock-Wadman, S.; Yuan, Y.; Brody, L. L.; Coulter, S. N.; Folger, K. R.; Kas, A.; Larbig, K.; Lim, R.; Smith, K.; Spencer, D.; Wong, G. K.; Wu, Z.; Paulsen, I. T.; Reizer, J.; Saier, M. H.; Hancock, R. E.; Lory, S.; Olson, M. V. Complete genome sequence of Pseudomonas aeruginosa PA01, an opportunistic pathogen. Nature (London) 2000, 406 (6799), 959-964.
6. Onifade, A. A.; Al-Sane, N. A.; Al-Musallam, A. A.; Al- Zarban, S. A review: Potentials for biotechnological applications of keratin-degrading microorganisms and their enzymes for nutritional improvement of feathers and other keratins as livestock feed resources. Bioresour. Technol. 1998, 66 (1), 1-11.
7. Edman, P.; Begg, G. A protein sequencer. Eur. J. Biochem. 1967, 1, 80-91.
8. Ausubel, F. M.; Brent, R.; Kingston, R. E.; Moore, D. D.; Seidman, J. G.; Smith, J. A.; Struhl, K. Current Protocols in Molecular Cloning; Greene Publishing and Wiley Interscience: New York, 1989.
9. Sambrook, J.; Fritsch, E. F.; Maniatis, T., Molecular Cloning, a Laboratory Manual; Cold Spring Harbor Laboratory Press: Cold Spring Harbor, NY, 1989; Vol. 1.
10. Hanahan, D.; Meselson, M. Plasmid screening at high colony density. Gene 1980, 10, 63-67.
11. Sangali, S.; Brandelli, A. Feather keratin hydrolysis by a Vibrio sp. strain kr2. J. Appl. Microbiol. 2000, 89 (5), 735-743.
12. Riffel, A.; Lucas, F.; Heeb, P.; Brandelli, A. Characterization of a new keratinolytic bacterium that completely degrades native feather keratin. Arch. Microbiol. 2003, 179 (4), 258-265.
13. Bradford, M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 1976, 72, 248-254.
14. Laemmli, U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 1970, 227 (5259), 680-685.
15. Doi, E.; Shibata, D.; Matoba, T. Modified colorimetric ninhydrin methods for peptidase assay. Anal. Biochem. 1981, 118 (1), 173-184.
16. Suntornsuk, W.; Tongjun, J.; Onnim, P.; Oyama, H.; Ratanakanokchai, K.; Kusamran, T.; Oda, K. Purification and characterisation of keratinase from a thermotolerant featherdegrading bacterium. World J. Microb. Biot. 2005, 21 (6-7), 1111-1117.
17. Lottenberg, R.; Christensen, U.; Jackson, C.M.; Coleman, P. L. Proteolytic enzymes: part C. In Methods in Enzymolology; Lorand, L., Ed.; Academic Press:New York, 1981; pp 341-361.
18. Lin, X.; Wong, S. L.; Miller, E. S.; Shih, J. C. Expression of the Bacillus licheniformis PWD-1 keratinase gene in B. subtilis. J. Ind. Microbiol. Biotechnol. 1997, 19 (2), 134-138.
19. Derman, A. I.; Prinz, W. A.; Belin, D.; Beckwith, J. Mutations that allow disulfide bond formation in the cytoplasm of Escherichia coli. Science 1993, 262, 1744-1747.
20. Bowden, G. A.; Georgiou, G. Folding and aggregation of β-lactamase in the periplasmic space of Escherichia coli. J. Biol. Chem. 1990, 265 (28), 16760-16766.
21. Takami, H.; Nogi, Y.; Horikoshi, K. Reidentification of the keratinase-producing facultatively alkaliphilic Bacillus sp. AH-101 as Bacillus halodurans. Extremophiles 1999, 3 (4), 293-296.
22. Nam, G. W.; Lee, D. W.; Lee, H. S.; Lee, N. J.; Kim, B. C.; Choe, E. A.; Hwang, J. K.; Suhartono, M. T.; Pyun, Y. R. Native-feather degradation by Fervidobacterium islandicum AW-1, a newly isolated keratinase-producing thermophilic anaerobe. Arch. Microbiol. 2002, 178 (6), 538-547.
23. Bressollier, P.; Letourneau, F.; Urdaci, M.; Verneuil, B. Purification and characterization of a keratinolytic serine proteinase from Streptomyces albidoflavus. Appl. Environ. Microbiol. 1999, 65 (6), 2570-2576.
24. Dozie, I.; Okeke, C.; Unaeze, N. A thermostable, alkalineactive, keratinolytic proteinase from Chrysosporium keratinophilum. World J. Microbiol. Biotechnol. 1994, 10, 563-567.
25. Bockle, B.; Galunsky, B.; Muller, R. Characterization of a keratinolytic serine proteinase from Streptomyces pactum DSM40530. Appl. Environ.Microbiol. 1995, 61 (10), 3705-3710.
26. Lee, H.; Suh, D. B.; Hwang, J. H.; Suh, H. J. Characterization of a keratinolytic metalloprotease from Bacillus sp. SCB-3. Appl. Biochem. Biotechnol. 2002, 97 (2), 123-133.
27. Werlang, P. O.; Brandelli, A. Characterization of a novel feather-degrading Bacillus sp. strain. Appl. Biochem. Biotechnol. 2005, 120 (1), 71-79.
28. Riessen, S.; Antranikian, G. Isolation of Thermoanaerobacter keratinophilus sp. nov., a novel thermophilic, anaerobic bacterium with keratinolytic activity. Extremophiles 2001, 5 (6), 399-408.
29. Sangali, S.; Brandelli, A. Isolation and characterization of a novel feather-degrading bacterial strain. Appl. Biochem. Biotechnol. 2000, 87 (1), 17-24.
30. Tatineni, R.; Doddapaneni, K. K.; Potumarthi, R. C.; Vellanki, R. N.; Kandathil, M. T.; Kolli, N.; Mangamoori, L. N. Purification and characterization of an alkaline keratinase from Streptomyces sp. Bioresour. Technol. 2008, 99 (6), 1596-1602.