Effects of different buffers on the thermostability and autolysis of a cold-adapted protease MCP-01

Journal of Protein Chemistry

Volumn 21, Issue 8, 2002, Pages 523-527

  Chen, Xiu Lan ^a   Sun, Cai Yun ^a   Zhang, Yu Zhong ^a   Gao, Pei Ji ^a  

^a SHANDONG UNIVERSITY   (China)

Author keywords

Autolysis; Buffer; Cold adapted protease; Thermostability

Indexed keywords

BORIC ACID; BUFFER; CARBONIC ACID; PHOSPHATE; PROTEIN MCP 01; PROTEINASE; UNCLASSIFIED DRUG;

ARTICLE; AUTOLYSIS; CAPILLARY ELECTROPHORESIS; CONFORMATIONAL TRANSITION; ENZYME ANALYSIS; ENZYME KINETICS; HYDROLYSIS; NONHUMAN; PROTEIN ANALYSIS; PROTEIN CONFORMATION; PSEUDOALTEROMONAS; TEMPERATURE DEPENDENCE; THERMOSTABILITY;

BORATES; CARBONATES; ELECTROPHORESIS, CAPILLARY; ENDOPEPTIDASES; HYDROGEN-ION CONCENTRATION; PHOSPHATES; PROTEIN CONFORMATION; PSEUDOALTEROMONAS; TEMPERATURE; TIME FACTORS; TROMETHAMINE;

BACTERIA (MICROORGANISMS); PSEUDOALTEROMONAS;

EID: 0036868198     PISSN: 02778033     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1022425621742     Document Type: Article

References (9)

1. Abraham, L. D., and Breuil, C. (1995). Biochim. Biophys. Acta 1245: 76-84.
2. Chen, X. L., Zhang, Y. Z., Wang, Y. T., Gao, P. J., and Luan, X. W. (2001). Marine Sci. 25: 4-8.
3. Feller, G., and Gerday, C. (1997). CMLS, Cell. Mol. Life Sci. 53: 830-841.
4. Gerday, C., Aittaleb, M., Arpigny, J. L., Baise, E., Chessa, J.-P., Garsoux, G., et al. (1997). Biochim. Biophysi. Acta 1342: 119-131.
5. Gerday, C., Aittaleb, M., Bentahir, M., Chessa, J. P., Claverie, P., et al. (2000). Tibtech 18: 103-107.
6. Hoshino, T., Ishizaki, K., Sakamoto, T., Kumeta, H., Yumoto, I., Matsuyama, H., et al. (1997). Lett. Appl. Microbiol. 25: 70-72.
7. Kumura, H., Murata, S., Hoshino, T., MiKawa, K., and Shimazaki, K. (1999). J. Dairy Sci. 82: 2078-2083.
8. Leiros, H.K., Willassen, N. P., and Smalas, A. O. (1999). Extremophiles 3: 205-219.
9. Shibata, M., Takahashi, S., Sato, R., and Oda, K. (1997). Biosci. Biotechnol. Biochem. 61: 710-715.