Optimized protocol for expression and purification of membrane-bound PglB, a bacterial oligosaccharyl transferase

Protein Expression and Purification

Volumn 89, Issue 2, 2013, Pages 241-250

  Jaffee, Marcie B ^a   Imperiali, Barbara ^a  

^a MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

Asparagine linked glycosylation; Membrane protein; Oligosaccharyl transferase; PglB; Purification protocol; Recombinant expression

Indexed keywords

DOLICHYL DIPHOSPHOOLIGOSACCHARIDE PROTEIN GLYCOTRANSFERASE; DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE - PROTEIN GLYCOTRANSFERASE; GLYCOSYLTRANSFERASE; MEMBRANE PROTEIN; RECOMBINANT PROTEIN;

AFFINITY CHROMATOGRAPHY; ARTICLE; CAMPYLOBACTER JEJUNI; CHEMICAL STRUCTURE; CHEMISTRY; ENZYME STABILITY; ENZYMOLOGY; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; GLYCOSYLATION; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; SOLUBILITY;

CAMPYLOBACTER JEJUNI; CHROMATOGRAPHY, AFFINITY; CLONING, MOLECULAR; ENZYME STABILITY; ESCHERICHIA COLI; GENE EXPRESSION; GLYCOSYLATION; HEXOSYLTRANSFERASES; MEMBRANE PROTEINS; MODELS, MOLECULAR; RECOMBINANT PROTEINS; SOLUBILITY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 84876976018     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2013.04.001     Document Type: Article

References (44)

1. M. Ahram, Z.I. Litou, R. Fang, and G. Al-Tawallbeh Estimation of membrane proteins in the human proteome In Silico Biol. 6 2006 379 386
2. J.P. Overington, B. Al-Lazikani, and A.L. Hopkins How many drug targets are there? Nat. Rev. Drug Discov. 5 2006 993 996
3. M.A. Yildirim, K.I. Goh, M.E. Cusick, A.L. Barabasi, and M. Vidal Drug-target network Nat. Biotechnol. 25 2007 1119 1126
4. F. Katzen, T.C. Peterson, and W. Kudlicki Membrane protein expression: no cells required Trends Biotechnol. 27 2009 455 460
5. C.A. Sarkar, I. Dodevski, M. Kenig, S. Dudli, and A. Mohr Directed evolution of a G protein-coupled receptor for expression, stability, and binding selectivity Proc. Natl. Acad. Sci. USA 105 2008 14808 14813
6. Q. Chen, L.J. Miller, and M. Dong Role of N-linked glycosylation in biosynthesis, trafficking, and function of the human glucagon-like peptide 1 receptor Am. J. Physiol. Endocrinol. Metab. 299 2010 E62 E68
7. F. Luthi, K. Leibundgut, F.K. Niggli, D. Nadal, and C. Aebi Serious medical complications in children with cancer and fever in chemotherapy-induced neutropenia: results of the prospective multicenter SPOG 2003 FN study Pediatr. Blood Cancer 59 2012 90 95
8. R. Ubelhart, M.P. Bach, C. Eschbach, T. Wossning, and M. Reth N-linked glycosylation selectively regulates autonomous precursor BCR function Nat. Immunol. 11 2010 759 765
9. S.L. Londrigan, S.G. Turville, M.D. Tate, Y.M. Deng, and A.G. Brooks N-linked glycosylation facilitates sialic acid-independent attachment and entry of influenza A viruses into cells expressing DC-SIGN or L-SIGN J. Virol. 85 2011 2990 3000
10. P.H. van Berkel, J. Gerritsen, G. Perdok, J. Valbjorn, and T. Vink N-linked glycosylation is an important parameter for optimal selection of cell lines producing biopharmaceutical human IgG Biotechnol. Prog. 25 2009 244 251
11. D. Bartusik, D. Aebisher, A.M. Lyons, and A. Greer Bacterial inactivation by a singlet oxygen bubbler: identifying factors controlling the toxicity of (1) O2 bubbles Environ. Sci. Technol. 46 2012 12098 12104
12. A. Deniaud, F. Bernaudat, A. Frelet-Barrand, C. Juillan-Binard, and T. Vernet Expression of a chloroplast ATP/ADP transporter in E. coli membranes: behind the Mistic strategy Biochim. Biophys. Acta 1808 2011 2059 2066
13. C. Lizak, Y.Y. Fan, T.C. Weber, and M. Aebi N-Linked glycosylation of antibody fragments in Escherichia coli Bioconjug. Chem. 22 2011 488 496
14. Z. Wei, T. Lin, L. Sun, Y. Li, and X. Wang N-linked glycosylation of GP5 of porcine reproductive and respiratory syndrome virus is critically important for virus replication in vivo J. Virol. 86 2012 9941 9951
15. J.K. Hurt, B.J. Fitzpatrick, J. Norris-Drouin, and M.J. Zylka Secretion and N-linked glycosylation are required for prostatic acid phosphatase catalytic and antinociceptive activity PLoS ONE 7 2012 e32741
16. M.L. Hillaire, M. van Eijk, N.J. Nieuwkoop, S.E. Vogelzang-van Trierum, and R.A. Fouchier The number and position of N-linked glycosylation sites in the hemagglutinin determine differential recognition of seasonal and 2009 pandemic H1N1 influenza virus by porcine surfactant protein D Virus Res. 169 2012 301 305
17. P. Somnuke, R.E. Hauhart, J.P. Atkinson, M.S. Diamond, and P. Avirutnan N-linked glycosylation of dengue virus NS1 protein modulates secretion, cell-surface expression, hexamer stability, and interactions with human complement Virology 413 2011 253 264
18. F. Schwarz, and M. Aebi Mechanisms and principles of N-linked protein glycosylation Curr. Opin. Struct. Biol. 21 2011 576 582
19. C. Lizak, S. Gerber, S. Numao, M. Aebi, and K.P. Locher X-ray structure of a bacterial oligosaccharyltransferase Nature 474 2011 350 355
20. M.B. Jaffee, and B. Imperiali Exploiting topological constraints to reveal buried sequence motifs in the membrane-bound N-linked oligosaccharyl transferases Biochemistry 50 2011 7557 7567
21. C. Guarino, and M.P. DeLisa A prokaryote-based cell-free translation system that efficiently synthesizes glycoproteins Glycobiology 22 2012 596 601
22. M.M. Chen, A.I. Bartlett, P.S. Nerenberg, C.T. Friel, and C.P. Hackenberger Perturbing the folding energy landscape of the bacterial immunity protein Im7 by site-specific N-linked glycosylation Proc. Natl. Acad. Sci. USA 107 2010 22528 22533
23. D.E. Taylor, M. Eaton, W. Yan, and N. Chang Genome maps of Campylobacter jejuni and Campylobacter coli J. Bacteriol. 174 1992 2332 2337
24. A.V. Karlyshev, J. Henderson, J.M. Ketley, and B.W. Wren An improved physical and genetic map of Campylobacter jejuni NCTC 11168 (UA580) Microbiology 144 Pt 2 1998 503 508
25. W.J. Bao, Y.G. Gao, Y.G. Chang, T.Y. Zhang, and X.J. Lin Highly efficient expression and purification system of small-size protein domains in Escherichia coli for biochemical characterization Protein Expr. Purif. 47 2006 599 606
26. F.W. Studier Protein production by auto-induction in high-density shaking cultures Protein Expr. Purif. 41 2005 207 234
27. K.J. Glover, E. Weerapana, S. Numao, and B. Imperiali Chemoenzymatic synthesis of glycopeptides with PglB, a bacterial oligosaccharyl transferase from Campylobacter jejuni Chem. Biol. 12 2005 1311 1315
28. M.M. Chen, K.J. Glover, and B. Imperiali From peptide to protein: comparative analysis of the substrate specificity of N-linked glycosylation in C. jejuni Biochemistry 46 2007 5579 5585
29. C.B. Sharma, L. Lehle, and W. Tanner N-Glycosylation of yeast proteins. Characterization of the solubilized oligosaccharyl transferase Eur. J. Biochem. 116 1981 101 108
30. S.M. Bhairi, C. Mohan, and E.M.D. Biosciences Detergents Booklet 2007 San Diego CA
31. S. Graslund, J. Sagemark, H. Berglund, L.G. Dahlgren, and A. Flores The use of systematic N- and C-terminal deletions to promote production and structural studies of recombinant proteins Protein Expr. Purif. 58 2008 210 221
32. N. Maita, J. Nyirenda, M. Igura, J. Kamishikiryo, and D. Kohda Comparative structural biology of eubacterial and archaeal oligosaccharyltransferases J. Biol. Chem. 285 2010 4941 4950
33. H. Xie, X.M. Guo, and H. Chen Making the most of fusion tags technology in structural characterization of membrane proteins Mol. Biotechnol. 42 2009 135 145
34. A.H. Davies, J.B. Jowett, and I.M. Jones Recombinant baculovirus vectors expressing glutathione-S-transferase fusion proteins Biotechnology (NY) 11 1993 933 936
35. K.D. Pryor, and B. Leiting High-level expression of soluble protein in Escherichia coli using a His6-tag and maltose-binding-protein double-affinity fusion system Protein Expr. Purif. 10 1997 309 319
36. D. Sachdev, and J.M. Chirgwin Solubility of proteins isolated from inclusion bodies is enhanced by fusion to maltose-binding protein or thioredoxin Protein Expr. Purif. 12 1998 122 132
37. T. Panavas, C. Sanders, and T.R. Butt SUMO fusion technology for enhanced protein production in prokaryotic and eukaryotic expression systems Methods Mol. Biol. 497 2009 303 317
38. F. Bernaudat, A. Frelet-Barrand, N. Pochon, S. Dementin, and P. Hivin Heterologous expression of membrane proteins: choosing the appropriate host PLoS ONE 6 2011 e29191
39. T. VanAken, S. Foxall-VanAken, S. Castleman, and S. Ferguson-Miller Alkyl glycoside detergents: synthesis and applications to the study of membrane proteins Methods Enzymol. 125 1986 27 35
40. P. Rosevear, T. VanAken, J. Baxter, and S. Ferguson-Miller Alkyl glycoside detergents: a simpler synthesis and their effects on kinetic and physical properties of cytochrome c oxidase Biochemistry 19 1980 4108 4115
41. QIAGEN, Technical Bulletin on Compatibility of reagents with Ni-NTA 06/2008.
42. J.P. Rosenbusch Stability of membrane proteins: relevance for the selection of appropriate methods for high-resolution structure determinations J. Struct. Biol. 136 2001 144 157
43. R. Grisshammer Understanding recombinant expression of membrane proteins Curr. Opin. Biotechnol. 17 2006 337 340
44. M.W. Lluis, J.I. Godfroy, and H. Yin Protein engineering methods applied to membrane protein targets Protein Eng. Des. Sel. 126 2012 91 100