Investigations on three genes in Ralstonia eutropha H16 encoding putative cyanophycin metabolizing enzymes

Applied Microbiology and Biotechnology

Volumn 97, Issue 8, 2013, Pages 3579-3591

  Adames, Katja ^a   Euting, Karina ^a   Bröker, Anna ^a   Steinbüchel, Alexander ^a,b  

^a UNIVERSITY OF MÜNSTER   (Germany)

^b KING ABDULAZIZ UNIVERSITY   (Saudi Arabia)

Author keywords

cphA; cphA ; cphB; Cyanophycin synthetase; Cyanophycinase; Poly(amino acids); Ralstonia eutropha

Indexed keywords

CPHA; CPHB; CYANOPHYCINASE; POLYAMINO ACIDS; RALSTONIA EUTROPHA; SYNTHETASES;

AMINO ACIDS; BACTERIA; CELLS; CYTOLOGY; ENCODING (SYMBOLS); ENZYME ACTIVITY; ESCHERICHIA COLI; GENES; LIGHT SCATTERING;

TRANSCRIPTION;

CPHA PROTEIN; CYANOPHYCIN; UNCLASSIFIED DRUG;

AMINO ACID; BACTERIUM; ENZYME ACTIVITY; FUNCTIONAL ROLE; GENE EXPRESSION; HOMOLOGY; LIGHT SCATTERING; METABOLISM; POLYMERASE CHAIN REACTION; PROTEIN;

ARTICLE; COMPUTER MODEL; CONTROLLED STUDY; CUPRIAVIDUS NECATOR; DELETION MUTANT; ENZYME ACTIVITY; HETEROLOGOUS EXPRESSION; NONHUMAN; PROTEIN METABOLISM; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CLONING, MOLECULAR; COMPUTATIONAL BIOLOGY; CUPRIAVIDUS NECATOR; ESCHERICHIA COLI; GENE EXPRESSION PROFILING; MOLECULAR SEQUENCE DATA; PEPTIDE HYDROLASES; PEPTIDE SYNTHASES; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SEQUENCE ALIGNMENT;

BACTERIA (MICROORGANISMS); CUPRIAVIDUS NECATOR; ESCHERICHIA COLI;

EID: 84876669686     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-012-4599-6     Document Type: Article

References (42)

1. Abd-El-Karem Y, Elbers T, Reichelt R, Steinbüchel A (2011) Heterologous expression of Anabaena sp. PCC 7120 cyanophycin metabolism genes cphA1 and cphB1 in Sinorhizobium (Ensifer) meliloti 1021. Appl Microbiol Biotechnol 89:1177-1192
2. Aboulmagd E, Oppermann-Sanio FB, Steinbüchel A (2000) Molecular characterization of the cyanophycin synthetase from Synechocystis sp. strain PCC6308. Arch Microbiol 174:297-306
3. Aboulmagd E, Voß I, Oppermann-Sanio FB, Steinbüchel A (2001) Heterologous expression of cyanophycin synthetase and cyanophycin synthesis in the industrial relevant bacteria Corynebacterium glutamicum and Ralstonia eutropha and in Pseudomonas putida. Biomacromolecules 2:1338-1342
4. Altschul SF, Madden TL, Schäffer AA, Zhang Z, Miller W, Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25:3389-3402
5. Anderson AJ, Dawes EA (1990) Occurrence, metabolism, metabolic role, and industrial uses of bacterial polyhydroxyalkanoates. Microbiol Rev 54:450-472
6. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248-254
7. Bullock WO, Fernandez JM, Stuart JM (1987) XL1-Blue: a high efficiency plasmid transforming recA Escherichia coli strain with β-galactosidase selection. Biotechniques 5:376-379
8. Caldara M, Dupont G, Leroy F, Goldbeter A, De Vuyst L, Cunin R (2007) Arginine biosynthesis in Escherichia coli. Experimental perturbation and mathematical modelling. J Biol Chem 283:6347-6358
9. Elbahloul Y, Krehenbrink M, Reichelt R, Steinbüchel A (2005) Physiological conditions conducive to high cyanophycin content in biomass of Acinetobacter calcoaceticus strain ADP1. Appl Environ Microbiol 71:858-866
10. Fleige C, Kroll J, Steinbüchel A (2011) Establishment of an alternative phosphoketolase-dependent pathway for fructose catabolism in Ralstonia eutropha H16. Appl Microbiol Biotechnol 91:769-776
11. Füser G, Steinbüchel A (2007) Analysis of genome sequences for genes of cyanophycin metabolism: identifying putative cyanophycin metabolizing prokaryotes. Macromol Biosci 7:278-296
12. Frey KM, Oppermann-Sanio FB, Schmidt H, Steinbüchel A (2002) Technical-scale production of cyanophycin with recombinant strains of Escherichia coli. Appl Environ Microbiol 68:3377-3384
13. Hai T, Frey KM, Steinbüchel A (2006) Activation of cyanophycin synthetase of Nostoc ellipsosporum strain NE1 by truncation at the carboxy-terminal region. Appl Microbiol Biotechnol 72:7652-7660
14. Hanahan D (1983) Studies on transformation of Escherichia coli with plasmids. J Mol Biol 166:557-580
15. Kohlmann Y, Pohlmann A, Otto A, Becher D, Cramm R, Lütte S, Schwartz E, Hecker M, Friedrich B (2011) Analyses of soluble and membrane proteomes of Ralstonia eutropha H16 reveal major changes in the protein complement in adaptation to lithoautotrophy. J Proteome Res 10:2767-2776
16. Kovach ME, Phillips RW, Elzer PH, Roop RM, Peterson KM (1994) pBBR1MCS: a broad-host-range cloning vector. Biotechniques 16:800-802
17. Kovach ME, Elzer PH, Hill DS, Robertson GT, Farris MA, Roop RM, Peterson KM (1995) Four new derivatives of the broad-host-range cloning vector pBBR1MCS, carrying different antibiotic-resistance cassettes. Gene 166:175-176
18. Krehenbrink M, Oppermann-Sanio FB, Steinbüchel A (2002) Evaluation of non-cyanobacterial genome sequences for occurrence of genes encoding proteins homologous to cyanophycin synthetase and cloning of an active cyanophycin synthetase from Acinetobacter sp. strain DSM 587. Arch Microbiol 177:371-380
19. Kroll J, Steinle A, Reichelt R, Ewering C, Steinbüchel A (2009) Establishment of a novel anabolism-based addiction system with an artificially introduced mevalonate pathway: complete stabilization of plasmids as universal application in white biotechnology. Metabol Eng 11:168-177
20. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685
21. Law AM, Lai SWS, Tavares J, Kimber MS (2009) The structural basis of β-peptide-specific cleavage by the serine protease cyanophycinase. J Mol Biol 392:393-404
22. Lin K, Elbahloul Y, Steinbüchel A (2012) Physiological conditions conductive to high cell density and high cyanophycin content in Ralstonia eutropha strain H16 possessing a KDPG aldolase gene-dependent addiction system. Appl Microbiol Biotechnol 93:1885-1894
23. Te gene expression. J Biotechnol 158:50-58
24. Peplinski K, Ehrenreich A, Döring C, Bömeke M, Reinecke F, Hutmacher C, Steinbüchel A (2010) Genome-wide transcriptome analyses of the "Knallgas" bacterium Ralstonia eutropha H16 with regard to polyhydroxyalkanoate metabolism. Microbiology (SGM) 156:2136-2152
25. Pohlmann A, Fricke WF, Reinecke F, Kusian B, Liesegang H, Cramm R, Eitinger T, Ewering C, Pötter M, Schwartz E, Strittmatter A, Voss I, Gottschalk G, Steinbüchel A, Friedrich B, Bowien B (2006) Genome sequence of the bioplastic-producing «Knallgas» bacterium Ralstonia eutropha H16. Nat Biotechnol 24:1257-1262
26. Pötter M, Müller H, Steinbüchel A (2005) Influence of homologous phasins (PhaP) on PHA accumulation and regulation of their expression by the transcriptional repressor PhaR in Ralstonia eutropha H16. Microbiology (SGM) 151:825-833
27. Quandt J, Hynes MF (1993) Versatile suicide vectors which allow direct selection for gene replacement in Gram-negative bacteria. Gene 127:15-21
28. Reinecke F, Steinbüchel A (2009) Ralstonia eutropha strain H16 as model organism for PHA metabolism and for biotechnological production of technically interesting biopolymers. J Mol Microbiol Biotechnol 16:91-108
29. Richter R, Hejazi M, Kraft R, Ziegler K, Lockau W (1999) Cyanophycinase, a peptidase degrading the cyanobacterial reserve material multi-l-arginyl-poly- l-aspartic acid (cyanophycin). Molecular cloning of the gene of Synechocystis sp. PCC 6803, expression in Escherichia coli, and biochemical characterization of the purified enzyme. Eur J Biochem 263:163-169
30. Sallam A, Steinbüchel A (2008) Anaerobic and aerobic degradation of cyanophycin by the denitrifying bacterium Pseudomonas alcaligenes strain DIP1 and role of three other coisolates in a mixed bacterial consortium. Appl Environ Microbiol 74:3434-3443
31. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
32. Schlegel HG, Gottschalk G, von Bartha R (1961) Formation and utilization of poly-β-hydroxybutyric acid by Knallgas bacteria (Hydrogenomonas). Nature 191:463-465
33. Simon RD, Weathers P (1976) Determination of the structure of the novel polypeptide containing aspartic acid and arginine which is found in cyanobacteria. Biochim Biophys Acta 420:165-176
34. Simon R, Priefert U, Pühler A (1983) A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in Gram negative bacteria. Biotechnology 1:784-791
35. Steinle A, Oppermann-Sanio FB, Reichelt R, Steinbüchel A (2008) Synthesis and accumulation of cyanophycin in transgenic strains of Saccharomyces cerevisiae. Appl Environ Microbiol 74:3410-3418
36. Steinle A, Steinbüchel A (2008) Cyanophycin synthetases. In: Lutz S, Bornscheuer UT (eds) Protein engineering handbook. Wiley-VCH Verlag, Weinheim, Germany, pp 829-848
37. Steinle A, Bergander K, Steinbüchel A (2009) Metabolic engineering of Saccharomyces cerevisiae for production of novel cyanophycins with an extended range of constituent amino acids. Appl Environ Microbiol 75:3437-3446
38. Steinle A, Witthoff S, Krause JP, Steinbüchel A (2010) Establishment of cyanophycin biosynthesis in Pichia pastoris and optimization by use of engineered cyanophycin synthetases. Appl Environ Microbiol 76:1062-1070
39. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucl Acids Res 24:4876-4882
40. Voß I, Diniz SC, Aboulmagd E, Steinbüchel A (2004) Identification of the Anabaena sp. strain PCC7120 cyanophycin synthetase as suitable enzyme for production of cyanophycin in Gram-negative bacteria like Pseudomonas putida and Ralstonia eutropha. Biomacromolecules 5:1588-1595
41. Voss I, Steinbüchel A (2006) Application of a KDPG-aldolase gene-dependent addiction system for enhanced production of cyanophycin in Ralstonia eutropha strain H16. Metabol Eng 8:66-78
42. Wilde E (1962) Untersuchungen über Wachstum und Speicherstoffsynthese von Hydrogenomonas eutropha. Arch Mikrobiol 43:109-137