Cyanophycinase, a peptidase degrading the cyanobacterial reserve material multi-L-arginyl-poly-L-aspartic acid (cyanophycin). Molecular cloning of the gene of Synechocystis sp. PCC 6803, expression in Escherichia coli, and biochemical characterization of the purified enzyme

European Journal of Biochemistry

Volumn 263, Issue 1, 1999, Pages 163-169

  Richter, Ralf ^a   Hejazi, Mahdi ^a   Kraft, Regine ^b   Ziegler, Karl ^a   Lockau, Wolfgang ^a  

^a HUMBOLDT UNIVERSITY   (Germany)

^b MAX DELBRÜCK CENTER FOR MOLECULAR MEDICINE   (Germany)

Author keywords

Cyanobacteria; Cyanophycinase; Dipeptidase E; Heterologous expression; Nonribosomal peptide synthesis

Indexed keywords

ARGININE; ASPARTIC ACID; BACTERIAL PROTEIN; CYANOPHYCIN; CYANOPHYCINASE; DIPEPTIDASE; PEPTIDASE; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL GENE; CYANOBACTERIUM; DEPOLYMERIZATION; ENZYME PURIFICATION; ENZYME SPECIFICITY; ENZYMOLOGY; ESCHERICHIA COLI; GENE EXPRESSION; HYDROLYSIS; HYDROPHOBICITY; MOLECULAR CLONING; NONHUMAN; PEPTIDE SYNTHESIS; PH; PRIORITY JOURNAL; PROTEIN DEGRADATION; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; TEMPERATURE;

AMINO ACID SEQUENCE; BASE SEQUENCE; CLONING, MOLECULAR; CYANOBACTERIA; DIPEPTIDASES; DNA PRIMERS; ESCHERICHIA COLI; GENE EXPRESSION; GENES, BACTERIAL; HYDROGEN-ION CONCENTRATION; MOLECULAR SEQUENCE DATA; PEPTIDE HYDROLASES; PLANT PROTEINS; PROTEASE INHIBITORS; RECOMBINANT PROTEINS; SALMONELLA TYPHIMURIUM; SEQUENCE HOMOLOGY, AMINO ACID; SUBSTRATE SPECIFICITY; TEMPERATURE;

EID: 0033167939     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.1999.00479.x     Document Type: Article

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